Structure of PDB 7xam Chain f Binding Site BS01

Receptor Information

>7xam Chain f (length=37) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

MKVNPSVKPICDKCRVIRRHGRVMVICSDPRHKQRQG

Ligand information

>7xam Chain A (length=3062)

aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugagacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaaccaggagguuggcuuagaagcagccacccuug
aaagagugcguaauagcucacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccaugggaccuucguugguaguagucaagcgauggggugac
gcaggaagguagccguaccggucagugguaauaccgggguaagccuguag
ggagucagauagguaaauccgucuggcauauauccugagaggugaugcau
agccgagugaggcgaauucggugauccuaugcugccgagaaaagccucua
gcgaggacauacacggcccguaccccaaaccaacacagguggucagguag
agaauacuaaggcguacgagugaacuaugguuaaggaacucggcaaaaug
cccccguaacuucgggagaagggggacccacauggcguguaagccuuuac
ggcccaagcgugaguggguggcacaaaccagugagaagcgacuguuuacu
aaaaacacagguccgugcgaagucgcaagacgauguauacggacugacgc
cugcccggugcuggaagguuaagaggacccguuaacucccuuugggggug
aagcggagaauuuaagccccaguaaacggcgguggucauccuaagguagc
gaaauuccuugucggguaaguuccgaccugcacgaauggcguaacgacuu
cucaacugucucaaccauagacucggcgaaauugcacuacgaguaaagau
gcucguuacgcgcggcaggacgaaaagaccccgggaccuucacuacaacu
ugguauuggugcucgauacgguuuguguaggauaggugggagacugugaa
gcucacacgccaguguggguggagucguuguugaaauaccacucugaucg
uauugggccucuaaccucggaccguauauccgguucagggacagugccug
guggguaguuuaacuggggcgguugccuccuaaaauguaacggaggcgcc
caaagguucccucaaccuggacggcaaucagguguugaguguaagugcac
aagggagcuugacugcgagacggacaugucgagcagggacgaaagucggg
acuagugauccggcaccucugaguggaaggggugucgcucaacggauaaa
agguaccccggggauaacaggcugaucuuccccaagaguccauaucgacg
ggaugguuuggcaccucgaugucggcucgucgcauccuggggcuggagca
ggucccaaggguugggcuguucgcccauuaaagcggcacgcgagcugggu
uuagaacgucgugagacaguucggucucuauccgccgcgcgcgucagaag
cuugaggaaaccugucccuaguacgagaggaccgggacggacgaaccucu
gguauaccaguugucccaccaggggcacggcuggauagccacguucggac
aggauaaccgcugaaagcaucuaagcgggaaaccucuuccaagaccaggc
uucucacccucuaggagggauaaggccccccgcagaccacgggauugaua
gaccagaccuggaagccuaguaauaggugcagggaacuggcacuaaccgg
ccgaaaacuuac

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<.........(((((....
.>>>>>>>>>>......<<<<..<.....<<<<<<<))))).>>>>>>><
<<<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.....>.
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<<..>>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<<<<....<<<<<<
....>>>>>.>....>>>>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<........<<<<<.<<<..<<<<.......>>>>..>>>..
.......<<......>>...>>>>>....>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<...<<<<<<<.
.....<<<<..>>>>.....>>>>>>>.....>>>>.<<...<<<<<...
<<<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<...<
<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...>>..
.<<<......>>>.....>>>>...>>>...>>>>........>>>>>..
>>....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.
....>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<.....
<<<<<.....<..>......>>>>><<<<<<...<<<<....<<<.....
>>>....>>>>..>>>>>>............<<<<<<<<<..<<<.....
....>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<
...<<....<<<..........<......<<...<<<<<<...>>>>>>.
...>>......>........>>>....>>.....................
..........<<<<<.......>>>>>...........>>>>..>>.>>>
>>>>..>..>>.>>>>>>>>>>>>>>>.....>.((..<<<<<......)
)>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<<<..<<<<<.<<<
.<<<<<.<<.<<<<<<<<<<<<.............<<<<..<<<......
<<<<<<<<....>>>>>>>>...>>>............>>>.>...>>>>
>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>>>....>>>>>.>
>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<
<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>..
..>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>
)))]......<<<<<<<.<<..>>>>..>>>>>>>>>.............
>>>>..>>>>>>.......<<<<.<.<.<<<<...<<.......>>...>
>>>.>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......
>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>.
...<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>>....<<<<<
<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<
<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>>>.>>>>>...
<<<.<..<<<<<...........>>>>>...>>>>.>>>>.......>>>
>>>>..<<<<<...>>>>>....<<<<.<<<<.......>>>>.....<<
<.<<<<.<<<....<<<<<....>>>>>...>>>..>>>>..>>>...>>
>>....>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7xam Cryo-EM structures reveal a unique conformational dynamics of the Mycobacterium 23S rRNA helices
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): M1 K2 P5 S6 V7 K8 R19 H20 R22 P30 R31 K33 Q36 G37
• Binding residue (residue number reindexed from 1): M1 K2 P5 S6 V7 K8 R19 H20 R22 P30 R31 K33 Q36 G37

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7xam, PDBe:7xam, PDBj:7xam
• PDBsum: 7xam
• PubMed: 37709237
• UniProt: A0QSL4|RL36_MYCS2 Large ribosomal subunit protein bL36 (Gene Name=rpmJ)