Structure of PDB 7ucj Chain f Binding Site BS01

Receptor Information

>7ucj Chain f (length=109) Species: 9986 (Oryctolagus cuniculus)

SGRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVY
KAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRI
RVMLYPSRI

Ligand information

>7ucj Chain 5 (length=3499)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggccgguccggccgugccggcggggcgga
ucucccgcucccgucggcgggggaccgccccccggccggcgaccggccgc
cgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacg
gcugggaaggccccggcggggaagguggcccgggggccccggguguuaca
gccccgccccggcagcagcagcucgccgaaucccggggccgagggagcga
gacccgugccgccgcgcucuccccccugggggggccgggccaccccuccc
acggcgcgaccgcuggggcggacuguccagugcgccccgggcgggucgcg
ccgucgggcccgggggccacggcgucccccgcgagcgcacggggucggcg
gcgaugucggcuacccacccgacccgucuugaaacacggaccaaggaguc
uaacacgugcgcgagucgggggcuacgaaagccgccguggcgcaaugaag
gugaaggccggcggcgccgccggccgaggugggaucccgaggccuuccag
uccgccgagggcgcaccaccggcccgucucgcccgccgcgccggggaggu
ggagcacgagcgcacguguuaggacccgaaagauggugaacuaugccugg
gcagggcgaagccagaggaaacucugguggagguccguagcgguccugac
gugcgaaucggucguccgaccuggguauaggggcgaaagacuaaucgaac
caucuaguagcugguucccuccaaaguuucccucaggauagcuggcgcuc
uccgcaguuuuauccgguaaagcgaaugauuagaggucuuggggccgaaa
cgaucucaaccuauucucaaacuuuaaauggguaagaagcccggcucgcu
gguguggagccgggcguggaaugcgagugccuagugggccacuuuuggua
agcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgcccga
ugccgacgcucaucagaccccagaaaagguguugguugauauagacagca
ggaccugccgaaucaacuagcccugaaaauggauggcgcuggagcgucgg
gcccauacccggccgucgcuggcagucggcagagcgagaaagagaguggc
gcggacgcuacgccgcgacgaguaggagggccgcugcggugagccuugaa
gccuagggcgcgggcccggguggagccgccgcaggugcagaucuuggugg
uaguagcaaauauucaaacgagaacuuugaaggccgaaguggagaagggu
uccaugugaacagcaguugaacaugggucagucgguccugagagaugggc
gagcgccguuccgaagggacgggcgauggccuccguugcccucagccgau
cgaaagggagucggguucagauccccgaauccggaguggcggagaugggc
gccgcgaggcguccagugcgguaacgcgaccgaucccggagaagccggcg
ggagccccggggagaguucucuuuucuuugugaagggcagggcgcccugg
aauggguucgccccgagagaggggcccgugccuuggaaagcgucgcgguu
ccggcggcguccggugagcucucgcuggcccuugaaaauccgggggagag
gguguaaaucucgcgccgggccguacccauauccgcagcaggucuccaag
gugaacagccucuggcauguuggaacaauguagguaagggaagucggcaa
gccggauccguaacuucgggauaaggauuggcucuaagggcuggcagccg
acuuagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaa
gcaucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccag
ugcucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcg
ggaguaacuaugacucucuuaagguagccaaaugccucgucaucuaauua
gugacgcgcaugaauggaugaacgagauucccacugucccuaccuacuau
ccagcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaa
gaagacccuguugagcuugacucuagucuggcacggugaagagacaugau
cguuuuuucacugacccggugaggcgggggggcgagccgggcucucgcuu
cuggcgccaagcgcccggccggccgggcgcgacccgcuccggggacagug
ccagguggggaguuugacuggggcgguacaccugucaaacgguaacgcag
guguccuaaggcgagcucagggaggacagaaaccucccguggagcagaag
ggcaaaagcucgcuugaucuugauuuucaguacgaauacagaccgugaaa
gcggggccucacgauccuucugaccuuuuggguuuuaagcaggagguguc
agaaaaguuaccacagggauaacuggcuuguggcggccaagcguucauag
cgacgucgcuuuuugauccuucgaugucggcucuuccuaucauugugaag
cagaauucaccaagcguuggauuguucacccacuaauagggaacgugagc
uggguuuagaccgucgugagacagguuaguuuuacccuacugaugaugug
uuguugccaugguaauccugcucaguacgagaggaaccgcagguucagac
auuugguguaugugcuuggcugaggagccaauggggcgaagcuaccaucu
gugggauuaugacugaacgccucuaagucagaaucccgcccaggcggaac
gauagcgccggagccucgguuggccucggauagccggucccccgccgggg
uccggugcggagagcccuucgucccgggaaacggggcggccggaaagggg
gccgcccucucgcccgucacgcaccgcacguucguggggaaccuggcgcu
aaaccauucguagacgaccugcuucugggucgggguuucguacguagcag
agcagcugcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<<....>>......
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..>>>>>..<<<<<<<<<....<<<<<<<<..<<<<<<<<....<<<<..
....>>>>.....((......<<<.....))>>>...>.>>>>>>>...>
>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>..
...(((((..<<<<<<)))))..]]]........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<<.<<...<<
<<<<<<.<<<.....<<<<<<<<<....<<<<<<<.<<<<<.<<<<<<<.
<<<<<<<<<......>>>>>>>>>>>>>>>>>>>>>>>>>...>>>..>>
>>>>>>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<
<<...<<..<<<..<<<<<<<......<<<<<<<<.>>>>>>>>......
.>>>>>>>..>>>.........>>>>>>..>>>>>>>>>>>.....<<..
...{.>>......<<<<<<.<<<<<<..>>>>>>.<<<<<<..<<<.<<<
<<<<<<<<<<<<<<<<<<<<.<<<<..>>>>.>>>>>>.>>>.>>>>>>>
>>>>.>>>...>>>>>>>.>>...........>>>>>>......>>.>>>
>>...>>>>>...>>>>>>>>..<<<<........>>>><<<<<<....<
<<<<<<<<<<<<..<<<...<<<<.....>>>>.....>>>...<<....
>>...<<<<<<<<<..>>>>>>>>>..<<<<<...<<<..<<<...(((.
[[.>>>..>>>....>>>>>.<<<<<<<<<.<<<<......>>>>>>>>>
>>.>>....>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<
<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.
.......>>>>.>>>....>>>>>>>>>...<<.......>>......>>
>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<.<<<<<<<
...<<<.<<<<<<<<<...<<<....<<<..<.<<<..<<<<.<......
...>.>>>>>>>.>..>>>..>.>>...>>>>>>>...<<<<<<<<<.<.
.....>>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<<<<<...
..>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(((...
....<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<
<<.>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>..
>>>...>>>>>>><<<<<<.<<<........}...........]].))).
...........>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<......
<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<
<....<<.....<<<<<<.......>>>>>>.<<<.....<<<<<.<<..
.<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<
....>>>..........<<.<<<<<<<<<...>>>>>>>>>>>......>
>....>>>..<<<<<........>>>>>))<<<<....<<<<<..<<<<<
<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<<<<<<
<<<<<<<<<<<<<<<<..{{{..>>>>>>....<<<<<.<<<<.<<<<..
...<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<....
...>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>>>>>>..>..
........>>.>>>.>>>>.>>>..>>.....<<......>>>>>>>.<<
<<.....>>>>..>>>....<<<<.....<<<<<<<.<<.........<.
....<<<<<.....<..>......>>>>><<.<<<<<<<<<<<.>>>>>.
.>>>>>>.>>............<<<<<<<<<..<<<.........>>>..
<<<<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<
<<<<<.....<<....<<<<<....>>>>.>...>>..>>>>..>>>>><
<<<<<.......>>>>>>........<...........<<<<<.......
>>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<........>>>>>....[[[[[..{{{.{{
......[[[[.{{[[[[..<<<<<.<<<<<<<<<.<<<<<<<<<<.....
.>>>>>>>>>>...<<<<<.....<<<<..<<.<<<<<>>.>>>>>....
..........<<<<<<<<<..>>>>>>>>>..>>>>..>>>>>....>>>
>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.((
(>>....>>>>>>>...<<<..<<<<.......>>>>..>>>.<<<....
>>>.>>)))]......((((((..(((..>>>>>>.))))))))).....
.....]]]]..}}]]]].......<<.<.<.<<<<<<...<<.......>
>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<.<<<<<.
.....>>>>>...>.>.<<<.........>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]].....<
<<<<<.<..<<<..<.<<<<<.................>>>>>.><<<<.
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><<<<<...<<<<<...........>>>>>...>>>>>.>>>...>.>>>
>>>.<<<<<<<..<<<<....<<<........<<<<<..<<<<<.>>>>>
.>>>>>.......>>>...<<<..<<<<....<<<<<<<<<........>
>>>>>.>>>...>>>>..>>>..............>>>>..>>.>>>>>.
<<<<<.<.............>....<<.<<<<<<<.<<.....<<<<.<.
.<<<<<.>>>>>..>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ucj Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): G3 R4 W6 K8 K15 R16 L18 R19 N20 Q21 E23 K29 Y34 V50 Y51 A53 K54 N55 T57 V58 T59 P60 T67 R68 V69 W71 K73 R76 H78 G79 N80 S81 R85 S90 N91 P93 A94 K95 R100 R102
• Binding residue (residue number reindexed from 1): G2 R3 W5 K7 K14 R15 L17 R18 N19 Q20 E22 K28 Y33 V49 Y50 A52 K53 N54 T56 V57 T58 P59 T66 R67 V68 W70 K72 R75 H77 G78 N79 S80 R84 S89 N90 P92 A93 K94 R99 R101

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ucj, PDBe:7ucj, PDBj:7ucj
• PDBsum: 7ucj
• PubMed: 35679869
• UniProt: G1SF08|RL35A_RABIT Large ribosomal subunit protein eL33 (Gene Name=RPL35A)