Structure of PDB 6tnn Chain f Binding Site BS01

Receptor Information

>6tnn Chain f (length=138) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

MLLPKRVKYRREHRGKMRGRAKGGTEVHFGEFGIQALEASWITNRQIEAA
RIAMTRYMKRGGKVWIKIFPSKPYTAKPLEVRMGSGKGAPEGWVAVVKPG
KVLFEISGVSEEVAREALRLASHKLPIKTKFVKREEIG

Ligand information

>6tnn Chain U (length=2930)

acgguuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccg
augaaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcu
uugauccggagauuuccgaauggggaaacccaccacucguaauggagugg
uauccauaucugaauucauaggauaugagaaggcagacccggggaacuga
aacaucuaaguacccggaggaagagaaagcaaaugcgauucccugaguag
cggcgagcgaaacgggaucagcccaaaccaagaggcuugccucuuggggu
uguaggacacucuguacggaguuacaaaggaacgagguagaugaagaggu
cuggaaaggcccgccauaggagguaacagcccuguagucaaaacuucguu
cucuccugaguggauccugaguacggcggaacacgugaaauuccgucgga
auccgggaggaccaucucccaaggcuaaauacucccuagugaccgauagu
gaaccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaa
agagauccugaaaccgugugccuacaaguagucagagcccguuaacgggu
gauggcgugccuuuuguagaaugaaccggcgaguuacgaucccgugcaag
guuaagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgc
augaguacguggucguagacccgaaaccaggugaucuacccauguccagg
gugaaguucagguaacacugaauggaggcccgaacccacgcacguugaaa
agugcggggaugagguguggguaggggugaaaugccaaucgaaccuggag
auagcugguucucuccgaaauagcuuuagggcuagccucaagguaagagu
cuuggagguagagcacugauuggacuaggggccccuaccggguuaccgaa
uucagucaaacuccgaaugccaaugacuuauccuugggagucagacugcg
agugauaagauccguagucgaaagggaaacagcccagaccgccagcuaag
gucccaaaguauacguuaaguggaaaaggauguggaguugcuuagacaac
caggauguuggcuuagaagcagccaccauuuaaagagugcguaauagcuc
acuggucgagugacucugcgccgaaaauguaccggggcuaaacguaucac
cgaagcugcggacuguucuucgaacagugguaggagagcguucuaagggc
ugugaagccagaccggaaggacugguggagcgcuuagaagugagaaugcc
gguaugaguagcgaaagaggggugagaauccccuccaccgaaugccuaag
guuuccugaggaaggcucguccgcucaggguuagucgggaccuaagccga
ggccgaaaggcguaggcgauggacaacagguugauauuccuguaccaccu
ccucaccauuugagcaauggggggacgcaggaggauaggguaagcgcggu
auuggauauccgcguccaagcaguuaggcugggaaauaggcaaauccguu
ucccauaaggcugagcugugauggcgagcgaaauauaguagcgaaguucc
ugauuccacacugccaagaaaagccucuagcgaggugagaggugcccgua
ccgcaaaccgacacagguaggcgaggagagaauccuaaggugaucgagag
aacucucguuaaggaacucggcaaaaugaccccguaacuucgggagaagg
ggugcucuguuagggugcaagcccgagagagccgcagugaauaggcccag
gcgacuguuuagcaaaaacacaggucucugcgaagccguaaggcgaagua
uaggggcugacgccugcccggugcuggaagguuaagaggagcgcuuagcg
uaagcgaaggugcgaauugaagccccaguaaacggcggccguaacuauaa
cgguccuaagguagcgaaauuccuugucggguaaguuccgacccgcacga
aaggcgcaacgaucugggcacugucucaacgagagacucggugaaauuau
aguaccugugaagaugcagguuacccgcgacaggacggaaagaccccgug
gagcuuuacugcagccugauauugaauguugguacagcuuguacaggaua
gguaggagccuuggaaaccggagcgccagcuuugguggaggcaucggugg
gauacuacccuggcuguauugaccuucuaacccgccgcccuuaucgggcg
gggagacagugucaggugggcaguuugacuggggcggucgccuccuaaaa
gguaacggaggcgcccaaagguucccucagaaugguuggaaaucauucgc
agaguguaaaggcacaagggagcuugacugcgagaccuacaagucgagca
gggacgaaagucgggcuuagugauccggugguuccgcauggaagggccau
cgcucaacggauaaaagcuaccccggggauaacaggcuuaucucccccaa
gaguccacaucgacggggagguuuggcaccucgaugucggcucaucgcau
ccuggggcuguagucggucccaaggguugggcuguucgcccauuaaagcg
guacgcgagcuggguucagaacgucgugagacaguucggucccuauccgu
cgcgggcgcaggaaauuugagaggagcuguccuuaguacgagaggaccgg
gauggacgcaccgcugguguaccaguuguucugccaagggcaucgcuggg
uagcuaugugcggacgggauaagugcugaaagcaucuaagcaugaagccc
cccucaagaugagauuucccauuccgcaaggaaguaagaucccugaaaga
ugaucagguugauaggucugagguggaaguguggcgacacauggagcuga
cagauacuaaucgaucgaggacuuaaccau

..<<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.
.....<<<<<<.....((.>>>.....<<<<<<<..<<.))......>>.
....>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>
>.<<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<
....>>......>>>>>.>.........<<....>>...<<<<......<
<.....>>.....>>>>...(((....<<<<<<<<....>>>>>>>>...
...<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<
<......>>>>.((......<<<.....))>>>...>>>...>>>>>>>>
>>>>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>..
....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]......
.........<<....>>....]].........<<<<....>>>>......
.......}....>>>>>>>>>>>.......<<<<..<<<<......>>>>
..>>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<
<<...<<<.....>>>...>>>...<<....>>..<<<<.....>>>>..
....>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<
<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<.......
.>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>..
.>..>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<
<<<<<.....<<<...<<<<<<<<......<<<<.....>>>>.......
>>>>>>>>..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<
.<<......>>.>>>>>>>....<<<......>>>...<<<<.<<<...<
<<(((.....<<<<<<...........<<..<<<<<<<<<........<<
<<<.<<.....................>>.........<<......>>..
.>>>>>....>>>>>>>>>.>>...........))).....>>>>>>.>>
>...>>>>>>><<<<<<<...>>>>>>>....>>>>>><.<<<<<<<.<<
......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>
>>><......<<....<<<<<<.......>>>>>><<<<....<<<<<.<
<<..<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<..
.<<<....>>>...>>>...>>...<<<<<........>>>>>))<<<<<
.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.<<<.<..
...{....>.>>>.<<..<......<<.<.<<<<<<<.<<.....>>>>>
>>>>.....>>>......>...>><..<<...........>>...>>.>>
>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>>>>>>.>
>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<..
..<<<<<<<<.<<<........<.....<<<<<....<<..>>.....>>
>>><<<<<....<<<<....>>>>...>>>>>............<<<<<<
<<<..<<<.........>>>..<<<<<<<.....<<<....>>>......
.>>>>>>>..<<<<.<<<..<<<<<<<..<<<...<<...<<<<<..<<.
...>>...>>>>>...>>...>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<.......>>>>>.....>..
..>>>>...>>.>>>>>>>..>.>>>.>>>>>>>>.>>>>>>.....>..
...<<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[
[<<<<..<<<<<.<<<<<<<<<.<<<.<<<..<<<<<<............
<<<<<..<<...............................>>........
....>>>>>..>.>>>>>..>>>.>>>....<<.<<<<<.......>>>>
>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<.....
......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>..
...<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>
>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>
>>.............>>>>..]]>>>>.......<<<<...<<<<<<...
<<.......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..
<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>
>>..>...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>
>>>>>>....<<<<<<<...<<<<..<<<<<<<...............>>
>>>>>.<<<<<...<<<<...<<<<<<<<<<<.}..>>>>>>..>>>>>.
..>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>
.>>>>.......>>>>>>>..<<<<....>>>>....<<<<<<<<<....
...>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..
>>>>>.>>>...>>>>....>>>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6tnn Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.
• Resolution: 3.07 Å
• Binding residue (original residue number in PDB): P4 R6 K8 H13 R14 R18 K22 G23 G24 H28 W41 R45 Q46 R56 W65 R82 M83 G84 S85 G86 K87 H123 K124
• Binding residue (residue number reindexed from 1): P4 R6 K8 H13 R14 R18 K22 G23 G24 H28 W41 R45 Q46 R56 W65 R82 M83 G84 S85 G86 K87 H123 K124

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6tnn, PDBe:6tnn, PDBj:6tnn
• PDBsum: 6tnn
• PubMed: 32991829
• UniProt: P14577|RL16_BACSU Large ribosomal subunit protein uL16 (Gene Name=rplP)