Structure of PDB 7osm Chain eL43 Binding Site BS01

Receptor Information

>7osm Chain eL43 (length=91) Species: 4932 (Saccharomyces cerevisiae)

AKRTKKVGITGKYGVRYGSSLRRQVKKLEIQQHARYDCSFCGKKTVKRGA
AGIWTCSCCKKTVAGGAYTVSTAAAATVRSTIRRLREMVEA

Ligand information

>7osm Chain 25S (length=3162)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucacacugggccagcauca
guuuugguggcaggauaaauccauaggaauguagcuugccucgguaagua
uuauagccugugggaauacugccagcugggacugaggacugcgacguaag
ucaaggaugcuggcaaugguuauaugccgcccgucuugaaacacggacca
aggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaa
ugaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgau
ccugaugucuucggauggauuugaguaagagcauagcuguugggacccga
aagauggugaacuaugccugaauagggugaagccagaggaaacucuggug
gaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguaua
ggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuu
cccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaauga
uuagagguuccggggugaccuugaccuauucucaaacuuuaaauauguaa
gaaguccuuguuacuuaauugaacguggacauuugaaugaagagcuuuua
gugggccauuuuugguaagcagaacuggcgaugcgggaugaaccgaacgu
agaguuaaggugccggaauacacgcucaucagacaccacaaaagguguua
guucaucuagacagccggacgguggccauggaagucggaauccgcuaagg
aguguguaacaacucaccggccgaaugaacuagcccugaaaauggauggc
gcucaagcguguuaccuauacucuaccgucaggguugauaugaugcccug
acgaguaggcaggcguggaggucagugacgaagccuagaccguaaggucg
ggucgaacggccucuagugcagaucuuggugguaguagcaaauauucaaa
ugagaacuuugaagacugaaguggggaaagguuccacgucaacagcaguu
ggacguggguuagucgauccuaagagauggggaagcuccguuucaaaggc
cugauuuuaugcaggccaccaucgaaagggaauccgguuaagauuccgga
accuggauauggauucuucacgguaacguaacugaauguggagacgucgg
cgcgagcccugggaggaguuaucuuuucuucuuaacagcuuaucaccccg
gaauugguuuauccggagauggggucuuauggcuggaagaggccagcacc
uuugcuggcuccggugcgcuugugacggcccgugaaaauccacaggaagg
aauaguuuucaugccaggucguacugauaaccgcagcaggucuccaaggu
gaacagccucuaguugauagaauaauguagauaagggaagucggcaaaau
agauccguaacuucgggauaaggauuggcucuaagggucgaacgaucaac
uuagaacugguacggacaaggggaaucugacugucuaauuaaaacauagc
auugcgauggucagaaagugauguugacgcaaugugauuucugcccagug
cucugaaugucaaagugaagaaauucaaccaagcgcggguaaacggcggg
aguaacuaugacucucuuaagguagccaaaugccucgucaucuaauuagu
gacgcgcaugaauggauuaacgagauucccacugucccuaucuacuaucu
agcgaaaccacagccaagggaacgggcuuggcagaaucagcggggaaaga
agacccuguugagcuugacucuaguuugacauugugaagagacauagagg
guaguggaaauuaccucuauguuucuuuacuuauucaaugaagcggagcu
ggaauucauuuuccacguucuagcauucaaggucccauucggggcugauc
cggguugaagacauugucagguggggaguuuggcuggggcggcacaucug
uuaaacgauaacgcagauguccuaaggggggcucauggagaacagaaauc
uccaguagaacaaaaggguaaaagcccccuugauuuugauuuucagugug
aauacaaaccaugaaaguguggccuaucgauccuuuagucccucggaauu
ugaggcuagaggugccagaaaaguuaccacagggauaacuggcuuguggc
agucaagcguucauagcgacauugcuuuuugauucuucgaugucggcucu
uccuaucauaccgaagcagaauucgguaagcguuggauuguucacccacu
aauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuua
cccuacugaugaauguuaccgcaauaguaauugaacuuaguacgagagga
acaguucauucggauaauugguuuuugcggcugucugaucaggcauugcc
gcgaagcuaccauccgcuggauuauggcugaacgccucuaagucagaauc
caugcuagaacgcggugauuucuuugcuccacacaauauagauggauacg
aauaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggug
cacuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauuu
ugcguggggauaaaucauuuguauacgacuuagauguacaacgggguauu
guaagcaguagaguagccuugguuacgaucugcugagauuaagccuuugu
ugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>...{.....<<....>>...<<<<......<<.....>>.....
>>>>...[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......>>
>>.(.......<<<......)>>>....>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]....}..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.......<<<<<<<<<<
<<<<<<<<<<<<<.....<<<<<<<<<....<.<<<<<<....>>>>>>>
......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>
>>....>>>>>>>>...>>>>>.>>>>>..<<<<........>>>><<<<
<<...<<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<
<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<
<<...<......>...>>>.>>>.>>....>>>>>>.>>>>>>>.<<.<.
.<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.
....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>.
..<<.......>>......>>>>>>...>..>.>>..<<<<<<....<.<
<<....>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<....<<<
....<<<.................>>>....>>>..>.>>.....>>>>>
...<<<<.<<<<.<......>>>>>.>>>>.....>>>>>.>>>>>>>>.
>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<
<<<<<..<<<<(((.....<<<<<<.....<<..........<<<.<<<<
<<<<<<.......<<<<<.<<....<<.........>>....>>......
...<<......>>...>>>>>...>>>>>>>>>>>>>..........)))
.>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>
>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<....>>>>>
>>>...>>.>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<
.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>
>.>>>>>>....((<<<<<<...<<....<<<....>>>...........
.....................>>....>>...<<<<<........>>>>>
))<<<<..<<<<<<.....<<<....>>>.......<<<<<<...<<<<.
<<<<<<<.<<<<<<<<<<..{{{..>>>>>><...<<<<<....<<<<<<
....<<<.....>>>....>>>>>>.....>>>>>....><<<<<<<<..
..>>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>......
....>>>..>>>.>>>>>>>>..>>.....<<......>>>>>>>.<<<<
.....>>>>..>>>>..<<<<.....<<<<<<<.<<..............
.<<<<<....<....>.....>>>>><<.<<<<<<<<<<....>>>>..>
>>>>>.>>............<<<<<<<<<..<<<.........>>>..<<
<<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<<
<<<....<<<....<<<<<....>>>>.>...>>>.>>>>..>>>>><<<
<<<.......>>>>>>........<...........<<<<<.......>>
>>>.....>.....>>....>>.>>>>>>>.....>>.>>>>>>>..>>>
>>>....>>....<<<<<........>>>>>....[[[[[..{{{.{{..
....[[[[.{{.[[[..<<<<<.<<<<<<<<..<<<<<<<<<<<<<<<<.
..............>>>>>>>>>>>>>>>>...<<<<<.....<<<<..<
<<<........>>>>...............<<<<<<....>>>>>>..>>
>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<
<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>>
>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<.......>
>>>.>>>>.<<<....>>>.>>))).......((((((..((((.>>>>>
>))))))))))..........]]]...}}]]]].......<<.<.<.<<<
<<<...<<.......>>...>>>>>>...>>.>.>.......<<<<<.<<
<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>..
...>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}.
.}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<.............
..>>>>>>.><<<<<...<<<<....<<<<<<<<<<}}}.>>>>>..>>>
>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>
>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....<<.....
.....<<<<<<.....>>>>>>......>>.......<<<<<<...<<<<
<.<..<<<......>>>..>.>>>>>.>>>>>>.............>>.>
>>.>>>>>>..<<<<<.<.............>.....<<<<<<<<<.<<<
....<<<<....<<<<<....>>>>>...>>>>...>>>....>>>>.>>
>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7osm Accuracy mechanism of eukaryotic ribosome translocation.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): A2 R4 T5 K6 K7 V8 G9 I10 G12 K13 Y14 G15 V16 R17 Y18 G19 S20 S21 R23 Q33 H34 F41 K44 R49 G50 A51 K62
• Binding residue (residue number reindexed from 1): A1 R3 T4 K5 K6 V7 G8 I9 G11 K12 Y13 G14 V15 R16 Y17 G18 S19 S20 R22 Q32 H33 F40 K43 R48 G49 A50 K61

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0046872 metal ion binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0044391 ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7osm, PDBe:7osm, PDBj:7osm
• PDBsum: 7osm
• PubMed: 34853469
• UniProt: P0CX25|RL43A_YEAST Large ribosomal subunit protein eL43A (Gene Name=RPL43A)