Structure of PDB 7nwg Chain e3 Binding Site BS01

Receptor Information
>7nwg Chain e3 (length=128) Species: 9986 (Oryctolagus cuniculus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
AALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKG
QILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAH
NVSSKNRKAIVERAAQLAIRVTNPNARL
Ligand information
>7nwg Chain 51 (length=3626) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcgcgguccggccgugccggcggggcgg
aucucccgcucccgucggcgggggaccgccccccggccggcgaccggccg
ccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggac
ggcugggaaggccccggcggggaagguggcccgggggccccggguguuac
agccccgccccggcagcagcagcucgccgaaucccggggccgagggagcg
agacccgugcgccgcgcucuccccccucgggggggccgggccaccccucc
cacggcgcgaccgcucucccacccggggggcggggcggacuguccccagu
gcgccccgggcgggucgcgccgucgggcccgggggagguucucucggggc
cacgcgagcgcacggggucggcggcgaugucggcuacccacccgacccgu
cuugaaacacggaccaaggagucuaacacgugcgcgagucgggggcuacg
aaagccgccguggcgcaaugaaggugaaggccggcggcgcgccgccggcc
gaggugggaucccgaggccuuccaguccgccgagggcgcaccaccggccc
gucucgcccgccgcgccggggagguggagcacgagcgcacguguuaggac
ccgaaagauggugaacuaugccugggcagggcgaagccagaggaaacucu
gguggagguccguagcgguccugacgugcgaaucggucguccgaccuggg
uauaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccaaa
guuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagcga
augauuagaggucuuggggccgaaacgaucucaaccuauucucaaacuuu
aaauggguaagaagcccggcucgcugguguggagccgggcguggaaugcg
agugccuagugggccacuuuugguaagcagaacuggcgcugcgggaugaa
ccgaacgccggguuaaggcgcccgaugccgacgcucaucagaccccagaa
aagguguugguugauauagacagcaggaugguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccugccgaaucaacuagcccugaaa
auggauggcgcuggagcgucgggcccauacccggccgucgcuggcagucg
gcagagcgagaaagagaguggcgcggacgcuacgccgcgacgaguaggag
ggccgcugcggugagccuugaagccuagggcgcgggcccggguggagccg
ccgcaggugcagaucuuggugguaguagcaaauauucaaacgagaacuuu
gaaggccgaaguggagaaggguuccaugugaacagcaguugaacaugggu
cagucgguccugagagaugggcgagcgccguuccgaagggacgggcgaug
gccuccguugcccucagccgaucgaaagggagucggguucagauccccga
auccggaguggcggagaugggcgccgcgaggcguccagugcgguaacgcg
accgaucccggagaagccggcgggagccccggggagaguucucuuuucuu
ugugaagggcagggcgcccuggaauggguucgccccgagagaggggcccg
ugccuuggaaagcgucgcgguuccggcggcguccggugagcucucgcugg
cccuugaaaauccgggggagaggguguaaaucucgcgccgggccguaccc
auauccgcagcaggucuccaaggugaacagccucuggcauguuggaacaa
uguagguaagggaagucggcaagccggauccguaacuucgggauaaggau
uggcucuaagggcuggcagccgacuuagaacuggugcggaccaggggaau
ccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguug
acgcgaugugauuucugcccagugcucugaaugucaaagugaagaaauuc
aaugaagcgcggguaaacggcgggaguaacuaugacucucuuaagguagc
caaaugccucgucaucuaauuagugacgcgcaugaauggaugaacgagau
ucccacugucccuaccuacuauccagcgaaaccacagccaagggaacggg
cuuggcggaaucagcggggaaagaagacccuguugagcuugacucuaguc
uggcacggugaagagacaugagagguguagaauaagugggaggcccgggc
cgccggugaaauaccacuacucugaucguuuuuucacugacccggugagg
cgggggggcgagccgggcucucgcuucuggcgccaagcgcccggccggcc
gggcgcgacccgcuccggggacagugccagguggggaguuugacuggggc
gguacaccugucaaacgguaacgcagguguccuaaggcgagcucagggag
gacagaaaccucccguggagcagaagggcaaaagcucgcuugaucuugau
uuucaguacgaauacagaccgugaaagcggggccucacgauccuucugac
cuuuuggguuuuaagcaggaggugucagaaaaguuaccacagggauaacu
ggcuuguggcggccaagcguucauagcgacgucgcuuuuugauccuucga
ugucggcucuuccuaucauugugaagcagaauucaccaagcguuggauug
uucacccacuaauagggaacgugagcuggguuuagaccgucgugagacag
guuaguuuuacccuacugaugauguguuguugccaugguaauccugcuca
guacgagaggaaccgcagguucagacauuugguguaugugcuuggcugag
gagccaauggggcgaagcuaccaucugugggauuaugacugaacgccucu
aagucagaaucccgcccaggcggaacgauagcgccggagccucgguuggc
cucggauagccggucccccgcccgggguccggugcggagagcccuucguc
ccgggaaacggggcggccggaaagggggccgcccucucgcccgucacgca
ccgcacguucguggggaaccuggcgcuaaaccauucguagacgaccugcu
ucugggucgggguuucguacguagcagagcagcugcugcgaucuauugaa
agucagcccucgacacaaggguuugu
.........................<<<<<<.....<......>......
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....>>>>.....((......<<<.....))>>>...>.>>>>>>>...>
>>..>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>..
...(((((..<<<<<<)))))..]]]........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<<.<....<<
<<<<<<.........<<<<<<<<<<....<<<<<<<.<<<...<<<<<<<
.<<<<<<<<<......>>>>>>>>>>>>>>>>..>>>>>>>...>>>.>>
>>>>>>>>..........>>>.>>>>>...<<<<<<<.<<<<<<<<<<.<
<<<.........<..<<<<<<<......<<<<<<<<.>>>>>>>>.....
..>>>>>>>...>............>>>>...>>>>>>>>>>.....<..
.......>.........<...<<<<<<..>>>>>>.....<<..<<<.<<
<<<<<<<<<<<<<<....<..<<..>>.>.<<<<<<<.<<........>>
.>>>>>>>.>>>.>>>>>>>>>>.>>>...>>>.............>>..
.......>.........>>.>>>>>....>>>>...>>>>>>>>...<<<
........>>>.<<<<<<....<<<.<<<<<<<<<..<<<...<<<<...
..>>>>.....>>>...<<....>>...<<<<<.<<<<..>>>>.>>>>>
..<<<<<...<<<..<<........[...>>..>>>....>>>>>.<<<<
<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>.>>>..<
<.<..<<<<<<<.....<<<<<<<<<..<<<....<.<<<<......>>>
>.>.....>>>...<<<..<..<<........>>.>.>>>....>>>>>>
>>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<...
.<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<<....<<<...
.<<<....<<<..<<<<.............>>>>>>>....>>>...>>>
....>>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>
>>>>>>.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......
>>>...<<<<<<<...<<<(((.......<<<<<.....<<.........
.<<<.<<<..<<<.........<<<.........<<.........>>...
............<<......>>.....>>>.....>>>..>>>>>>....
......))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<.....
................]................>>>>>>>>>....>>>>
>>.<<<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>
>>>>>>.>>.....>>>>>><<<....<<.....<<<<<<.......>>>
>>>.<<<......<<<<.<<...<<<<<<...<<.....>>.>>>>>>..
..((<<<<<<...<<....<<<....>>>.............<<<<<<<<
<...>>>>>>>>>........>>....>>>..<<<<<........>>>>>
))<<<<....<<<......<<<<<......>>>>>....<<<....>>>.
......<<<<<<...<<<<<<<<<<<<<<<<<<<<<<<..{{{..>>>>>
>.....<<<<.<<<<.<<<<.....<<<.....>>>.....>>>>>>>>.
.>>>>......<<<<<<<.......>>>>>>>>>>>.>.>>>>>>>>>>>
>.........>>>>>>.................>>>.>>>>.>>>..>>.
....<<......>>>>>>..<<<<.....>>>>..>>>....<<<<....
.<<<<<<<.<<...............<<<<<...............>>>>
><<.<<<<<<<<<<<.>>>>>..>>>>>>.>>............<<<<<<
<<<..<<...........>>..<<<<<<<....<<<<.....>>>>....
..>>>>>>>....<..<<<..<<<<<<....<<<.....<<<<....>>>
>.....>>>.>>>>..>>>>>...<<.........>>...........<.
..........<<<<<.......>>>>>.....>.....>.....>>.>>>
>>>>.....>>.>>>>>>>..>>>>>>...>>>....<<<<.........
.>>>>.....[[[[..{{{.{{......[[[[....[[[..<<<<..<<<
<<<<<<.<<<<<<....<<.<<<<..........<<<.<...<<<..>>>
.............>.>>>.>>>>.>>.....>>>>>>...<<<<<.....
<<<<.....<........>..................<<<<<<<<..>>>
>>>>>...>>>>..>>>>>....>>>>>>>>>...>>>>...<<<.....
>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<
<.......>>>>>>.....<<.[.(((>>....>>>>>>>...<<<.<<<
<<.......>>>>.>>>>.<<<....>>>.>>)))]......((((((..
(((..>>>>>>.)))))))))..........]]].....]]]].......
<<.<.<.<<<<<<...<.........>...>>>>>>...>>.>.>.....
..<<<<<.<<<<<...<...<<<<<......>>>>>.....>.<<<....
.....>>>......>>>>..>...>>>.>>....<<<.<<<....>>>..
>>>....}}..}}}.]]]]......<<<<<<....<<<..<.<<<<<...
..............>>>>>.><<<<.....<<<....<<..<.<<<.}}}
..>>>....>.>>...>>>....>>>><<<<....<<<<...........
..>>>>....>>>>.>>>.....>>>>>>.<<<<<<<..<<<.....<<<
........<<<<<<..<<<<..>>>>>.>>>>>.......>>>...<<<.
.<<<<....<<<.<<<<<........>>>>>..>>>...>>>>..>>>..
.............>>>..>>.>>>>>.<<<<<.<.............>..
..<<.<<<<<<<.<<.....<<<<.<..<<<<<.>>>>>..>>>>>....
>>....>>>>.>>>.>>..>>>>>..
Receptor-Ligand Complex Structure
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PDB7nwg Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.
Resolution3.8 Å
Binding residue
(original residue number in PDB)
K15 R16 T17 K18 K19 Q24 D26 R27 Y28 K30 I31 W35 R36 K37 D42 N43 R44 R46 R47 R48 K50 Q52 I58 S62 N63 H68 K83 E98 H101 N102 S104 K106 N124 N126 A127
Binding residue
(residue number reindexed from 1)
K14 R15 T16 K17 K18 Q23 D25 R26 Y27 K29 I30 W34 R35 K36 D41 N42 R43 R45 R46 R47 K49 Q51 I57 S61 N62 H67 K82 E97 H100 N101 S103 K105 N123 N125 A126
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003735 structural constituent of ribosome
Biological Process
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005840 ribosome
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function

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Biological Process

View graph for
Cellular Component
External links
PDB RCSB:7nwg, PDBe:7nwg, PDBj:7nwg
PDBsum7nwg
PubMed34157102
UniProtG1TUN8|RL32_RABIT Large ribosomal subunit protein eL32 (Gene Name=RPL32)

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