Structure of PDB 8wif Chain e Binding Site BS01

Receptor Information
>8wif Chain e (length=200) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
ARYTGPATRKSRRLGVDLVGGDQSFEKRPYPPGQHGRARIKESEYRQQLQ
EKQKARFSYGVMEKQFRRYYEEANRQPGKTGDNLLRILESRLDNVVYRAG
LARTRRMARQLVSHGHFLVNGVKVDIPSYRVSQYDIIDVKEKSLNTLPFQ
IARETAGERPIPSWLQVVGERQRILVHQLPERAQIDVPLTEQLIVELYSK
Ligand information
>8wif Chain a (length=1517) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uuuggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaac
acaugcaagucgaacggaaaggcccuuucggggguacucgaguggcgaac
gggugaguaacacgugggugaucugcccugcacuuugggauaagccuggg
aaacugggucuaauaccgaauacacccugcuggucgcauggccugguagg
ggaaagcuuuugcggugugggaugggcccgcggccuaucagcuuguuggu
ggggugauggccuaccaaggcgacgacggguagccggccugagaggguga
ccggccacacugggacugagauacggcccagacuccuacgggaggcagca
guggggaauauugcacaaugggcgcaagccugaugcagcgacgccgcgug
agggaugacggccuucggguuguaaaccucuuucagcacagacgaagcgc
aagugacgguaugugcagaagaaggaccggccaacuacgugccagcagcc
gcgguaauacguaggguccgagcguuguccggaauuacugggcguaaaga
gcucguaggugguuugucgcguuguucgugaaaacucacagcuuaacugu
gggcgugcgggcgauacgggcagacuagaguacugcaggggagacuggaa
uuccugguguagcgguggaaugcgcagauaucaggaggaacaccgguggc
gaaggcgggucucugggcaguaacugacgcugaggagcgaaagcgugggg
agcgaacaggauuagauacccugguaguccacgccguaaacgguggguac
uagguguggguuuccuuccuugggauccgugccguagcuaacgcauuaag
uaccccgccuggggaguacggccgcaaggcuaaaacucaaaggaauugac
gggggcccgcacaagcggcggagcauguggauuaauucgaugcaacgcga
agaaccuuaccuggguuugacaugcacaggacgccggcagagaugucggu
ucccuuguggccugugugcagguggugcauggcugucgucagcucguguc
gugagauguuggguuaagucccgcaacgagcgcaacccuugucucauguu
gccagcacguuaugguggggacucgugagagacugccggggucaacucgg
aggaagguggggaugacgucaagucaucaugccccuuauguccagggcuu
cacacaugcuacaauggccgguacaaagggcugcgaugccgugaggugga
gcgaauccuuucaaagccggucucaguucggaucggggucugcaacucga
ccccgugaagucggagucgcuaguaaucgcagaucagcaacgcugcggug
aauacguucccgggccuuguacacaccgcccgucacgucaugaaagucgg
uaacacccgaagccgguggccuaacccuuguggagggagccgucgaaggu
gggaucggcgauugggacgaagucguaacaagguagccguaccggaaggu
gcggcuggaucaccucc
......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<
<<.<<<..<<<..<<.<..<<<<<<<....>>>>>.>>>..>>>>>....
..<<........<<<<<<<..<<...<<<<<<<<<<<<.....<<<<<<.
...>>>>>>......>>>>.....<<<<<<<<<<<<..>>>>>.>>>>>>
>....<<....>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<
<<<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>..
.>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>
>>>.........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<
<<<......<<<<....>>>>.....>>>>...<.<<<<<......<<..
..>>.......>>>>>....>..<<<<<(((...<<<<<.....<<.)))
>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((.....
<<<<...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<<.....>>>>
>>>...>>>>>>>..>>>>>>>>>>...<<<<<<<<...<<<<<<<....
<<<<<<<<<..<<<......>>>.....>>>>>>>>>...........<<
....>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<..
.<<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<
..<<<<<<<<<<<<.......>>>>>>>>>>>>...<<..))>>.....>
>>>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<
<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>.
.........<<<<<<<......<<<<<<<...<<<<<<<....>>>>>>>
.<<.....>>.>>>>>>>.<<<.<<<..<<<<<<<......<<<<<<<<<
....>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<...
.<<..<<<......>>>>>.....>>>>>>.....<<<<<.....>>>>>
........>>>>.......>.>>>...>>>>>>>>>...>>>>>>>...>
>.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..
>>....>>>......>>>>>>>......<....<<<<<<<........>>
>>>>>....>.....>>>>>>....<<<<<<<..........>>>>>>>.
.....>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<
<..<<<<....<.<<<<..<<<..<<.....>>.>>>.>>>>.>...>>>
>..>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>
>>>>>>...........
Receptor-Ligand Complex Structure
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PDB8wif Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
Resolution2.9 Å
Binding residue
(original residue number in PDB)
A2 R3 P7 T9 R10 R13 R14 R29 P33 G34 Q35 H36 K42 Y46 K53 Q54 M63 E64 K65 Q66 N75 R76 R104 T105 R107 M108 R110 Q111 S114 H115 H117 V125 D126 I127 S129 Y130 P149 E197 S200 K201
Binding residue
(residue number reindexed from 1)
A1 R2 P6 T8 R9 R12 R13 R28 P32 G33 Q34 H35 K41 Y45 K52 Q53 M62 E63 K64 Q65 N74 R75 R103 T104 R106 M107 R109 Q110 S113 H114 H116 V124 D125 I126 S128 Y129 P148 E196 S199 K200
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0006412 translation
GO:0042274 ribosomal small subunit biogenesis
Cellular Component
GO:0005840 ribosome
GO:0015935 small ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:8wif, PDBe:8wif, PDBj:8wif
PDBsum8wif
PubMed38245551
UniProtA0QSL7|RS4_MYCS2 Small ribosomal subunit protein uS4 (Gene Name=rpsD)

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