Structure of PDB 8qcq Chain e Binding Site BS01

Receptor Information

>8qcq Chain e (length=158) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

LELEERLVTVNRVAKVVKGGRRFRFAALVVVGDKNGHVGFGTGKAQEVPE
AIRKAVEDAKKNLIEVPMVGTTIPHEIIGRFGAGNILLKPASEGTGVIAG
GPVRAVLELAGVADILSKSLGSNTPINMIRATLQGLSELKRAEDVAKLRG
KSVEELLG

Ligand information

>8qcq Chain a (length=1533)

ggagaguuugauccuggcucaggacgaacgcuggcggcgugccuaauaca
ugcaagucgagcggacagaugggagcuugcucccugauguuagcggcgga
cgggugaguaacacguggguaaccugccuguaagacugggauaacuccgg
gaaaccggggcuaauaccggaugguuguuugaaccgcaugguucaaacau
aaaagguggcuucggcuaccacuuacagauggacccgcggcgcauuagcu
aguuggugagguaacggcucaccaaggcgacgaugcguagccgaccugag
agggugaucggccacacugggacugagacacggcccagacuccuacggga
ggcagcaguagggaaucuuccgcaauggacgaaagucugacggagcaacg
ccgcgugagugaugaagguuuucggaucguaaagcucuguuguuagggaa
gaacaagugccguucgaauagggcgguaccuugacgguaccuaaccagaa
agccacggcuaacuacgugccagcagccgcgguaauacguagguggcaag
cguuguccggaauuauugggcguaaagggcucgcaggcgguuucuuaagu
cugaugugaaagcccccggcucaaccggggagggucauuggaaacugggg
aacuugagugcagaagaggagaguggaauuccacguguagcggugaaaug
cguagagauguggaggaacaccaguggcgaaggcgacucucuggucugua
acugacgcugaggagcgaaagcguggggagcgaacaggauuagauacccu
gguaguccacgccguaaacgaugagugcuaaguguuaggggguuuccgcc
ccuuagugcugcagcuaacgcauuaagcacuccgccuggggaguacgguc
gcaagacugaaacucaaaggaauugacgggggcccgcacaagcgguggag
caugugguuuaauucgaagcaacgcgaagaaccuuaccaggucuugacau
ccucugacaauccuagagauaggacguccccuucgggggcagagugacag
guggugcaugguugucgucagcucgugucgugagauguuggguuaagucc
cgcaacgagcgcaacccuugaucuuaguugccagcauucaguugggcacu
cuaaggugacugccggugacaaaccggaggaagguggggaugacgucaaa
ucaucaugccccuuaugaccugggcuacacacgugcuacaauggacagaa
caaagggcagcgaaaccgcgagguuaagccaaucccacaaaucuguucuc
aguucggaucgcagucugcaacucgacugcgugaagcuggaaucgcuagu
aaucgcggaucagcaugccgcggugaauacguucccgggccuuguacaca
ccgcccgucacaccacgagaguuuguaacacccgaagucggugagguaac
cuuuuaggagccagccgccgaaggugggacagaugauuggggugaagucg
uaacaagguagccguaucggaaggugcggcugg

...<<<<..[.((((.>>>>.<<<<.<<<<<...<<<<<<<.....<<<.
<<<..<<<..<<.<<<<...<<<..<..>..>>>...>>>>.>>>>>...
...<<........<<<<<....<<...<<<<<<<.<<<<......<<<<<
....>>>>>.......>>>>.....<<<<<<<<<<....>>>>>>>>>>.
....<<<<<......>>>>>.>>>>>>>..>>.>>.>>>.<<<....<<<
..<<<<<<<<.......>>>>>>>>>>>......>>>..<<<<<<<<...
.>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>
>...>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>
>>>...<<<<..<....<.<...>..>....>.>>>>...<<<<<<<...
......<<<<<<.<........>>>>>>>..........>>>>>.>..>.
.<<<<<((....<<<<<.....<<..))>>.......>>>>>>>>>>..>
>>>>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<
<<<<<<.......<<<<<<.....>>>>>>.....>>>>>>>..>>>>>>
>>>...<<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>
>......>>>>>>>>...........<<....>>.>>>>>>>..>>>>>.
>>>...>>>...>>>>....<<<<<<...<<...<<<<.........>>>
>...>>>>>>>>..........<<<<<<..<<<<<<<<<<<<......>>
>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<......<<<<
....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<
<<<<<<<<......<<........>>..........<<<<<<<......<
<<<<<<....<<<<<....>>>>>...<<<<...>>>.>>>>>>.>>.<<
<.<<<..<<<<<<.......<<<<<<<<<....>>>..<<<<......>>
>>..>>>>>>.....<<<<.<<<<<<<..<<<..<<......>>>>>...
>>>>>>>.....<<<<<.....>>>>>........>>>>.........>>
>...>>>>>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.
....<<<..<<..<<<<....>>>>..>>....>>>.....>>>>>>>..
....<....<<<<<<<........>>>>>>>....>.....>>>>>>...
.<<<<<<<.........>>>>>>>......>>...>>>>>>>>>>.>>..
..<..<<.<.<<<<.<<<..<<<<<<..<<<<....<.<<<<..<<<..<
<<...>>>.>>>.>>>>.>...>>>>..>>>>>>..>>>.>>>>..>.>>
...>.....<<<<<<<<<....>>>>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8qcq Novel arrest peptides induce ribosome stalling by short circuiting the ribosomal peptidyltransferase activity
• Resolution: 2.3 Å
• Binding residue (original residue number in PDB): V21 A22 K23 V24 V25 K26 G27 R29 R32 Q54 E58 R61 F89 G90 A91 L95 K97 S100 T103 I106 A107 G108 R112 L124 S125 K126 S127 L128 S130 N131
• Binding residue (residue number reindexed from 1): V13 A14 K15 V16 V17 K18 G19 R21 R24 Q46 E50 R53 F81 G82 A83 L87 K89 S92 T95 I98 A99 G100 R104 L116 S117 K118 S119 L120 S122 N123

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8qcq, PDBe:8qcq, PDBj:8qcq
• PDBsum: 8qcq
• PubMed: 38503735
• UniProt: P21467|RS5_BACSU Small ribosomal subunit protein uS5 (Gene Name=rpsE)