Structure of PDB 7ohu Chain e Binding Site BS01

Receptor Information

>7ohu Chain e (length=125) Species: 559292 (Saccharomyces cerevisiae S288C)

LPHPKIVKKHTKKFKRHHSDRYHRVAENWRKQKGIDSVVRRRFRGNISQP
KIGYGSNKKTKFLSPSGHKTFLVANVKDLETLTMHTKTYAAEIAHNISAK
NRVVILARAKALGIKVTNPKGRLAL

Ligand information

>7ohu Chain 1 (length=1692)

guuugaccucaaaucagguaggaguacccgcugaacuuauaucaauaagc
ggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagcgg
caaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaauuu
ggagagggcacuuuggggccguuccuugucuauguuccuuggaacaggac
gucauagagggugagaaucccguguggcgaggagugcgguucuuuguaaa
gugccuucgaagagucgaguuguuugggaaugcagcucuaaguggguggu
uccaucuaaagcuaaauauuggcgagagaccgauagcgaacaaguacagu
gauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacgugaaa
uuguugaaagggaagggcauuugaucagacaugguguuuuguggggaauc
ucgcauuucacugggccagcaucaguuuugguggcaggauaaauccauag
gaauguagcuugccucgguaaguauuauagccugugggaauacugccagc
ugggacugaggacugcgacguaagucaaggaugcuggcauaaugguuaua
ugccgccgucuugaaacacggaccaagggucuaacgucuaugcgaguguu
uggguguaaaacccauacgcguaaugaaagugaacguagguuggggccuc
gcaagaggugcacaaucgaccgauccugaggauggauuugaguaagagca
uagcuguugggacccgaaagauggugaaaccaucuaguacugguuccugc
cgaaguuucccucaggauagcagaagcucguaucaguuuuauuaaaauga
agagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggaug
aaccgaacguagaguuaaggugccggaauacacgcucaucagaccaaaag
guguuaguucaucuagacagccggacgguggccauggaagucggaauccg
cuaaggaguguguaacaacucaccggccgaaugaacuagcccugaauggc
gcucaagcguguuaccuauacucuaccgucaggguugauaugaugcccug
acgaguaggcaggcguggaggucagugacgaagccuagaccguaaggucg
ggucgaacggccucuagugagaucuuggugguaguagcagcgaaaccaca
gccaagggacgggcuuggcagaaucagcgggggcuuguggcagucaagcg
gcgacauugcucauaccgaagcagaauucgguaagcgucuacugaugaug
uuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggau
aauugguuuuugcggcugucugaucaggcagccgcgaagcuaccauccgc
uggauuauggcugaacgccucuaagucagauccaugcuagaacgcgguga
uuucuuugcuccacacaauauagauggauacgaauaaggcguccuugugg
cgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccu
ugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaaucau
uuguauacgacuuagauguacaacgggguauuguaagcaguagaguagcc
ugguuacgaucugcugagauuaaccuuuguugucugauuugu

..........................<<<<<<...............>>>
>>.>.........<<....>>..<<<<<......<<.....>>.....>>
>>>..<<<..<........<<.<<<<<<...>>>>>>.>>........>.
...<<<<<<<<<<..<<<<<<<<<<<<<<<.....<<<<......>>>>.
((......<<<.....))>>>....>>>>>>>>..>>>>>>>......>>
>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<<<.
.>>>>>>..>>>..................................<<..
..>>...............<<<<....>>>>...................
...............<<<<<.<<<<<.<....<<<<...<<<........
..>>>...>>>>..<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<
<......<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>
>>>>>>>>........<<<....>>>.....>>>>>>>....>>>>>>.>
>>>>.<<<<........>>>><<<<<<...<<<<<<.<<<<<<..<<<..
.<<<<.....>>>>...>>>..................<<<<<..<<<<<
....>>>>>...>>>>>.<<<<<<<<.......>>>.>>>.>>....>>>
>>>.>>>>>>..<<.<...<<<<<<...>>>>>>.....>.>>..<<<<<
<....<.<<......>>.><<<<<<<<<....<<<.<<<<...>>>>>>>
..>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<....
..>>>...<<<<<<<..<<<<(((.....<<<<<<......<......<<
<.<<<<<<<<<<.......<<<<<.<<....<<.........>>....>>
.........<<......>>...>>>>>...>>>>>>>>>>>>>....)))
.>....>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>
>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>....>>>>>><<.....<<.>>....>>...
.<<<<<.......>>>>>....<<<<<..<.........<<<<....<<.
>>....>>>>...<<<<<<<......>>>>>>>......>.>>>>>...<
<<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<
...<<......<<<<<<<<........>>>>>>>>.....>>..>>>>>.
<<<<...<<<<<...........>>>>>..>>>>.>>>>....>>>>>>>
>.......<<<<<<.<<<.<<....<<..........<<<<<<.....>>
>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>.
.>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<
.............>.....<<<<<<<<<.<<<....<<<<....<<.<<.
..>>.>>...>>>>...>>>...>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohu Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): L4 K11 K12 K16 R19 D23 R24 H26 R27 V28 N31 W32 R33 S40 R43 R44 R45 F46 R47 G48 P53 K54 I55 G56 Y57 G58 S59 N60 K61 K64 F65 L75 E95 A97 H98 N99 I100 S101 A102 K103 N104 R105 N121 P122 R125
• Binding residue (residue number reindexed from 1): L1 K8 K9 K13 R16 D20 R21 H23 R24 V25 N28 W29 R30 S37 R40 R41 R42 F43 R44 G45 P50 K51 I52 G53 Y54 G55 S56 N57 K58 K61 F62 L72 E92 A94 H95 N96 I97 S98 A99 K100 N101 R102 N118 P119 R122

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohu, PDBe:7ohu, PDBj:7ohu
• PDBsum: 7ohu
• PubMed: 34813592
• UniProt: P38061|RL32_YEAST Large ribosomal subunit protein eL32 (Gene Name=RPL32)