Structure of PDB 7bl5 Chain e Binding Site BS01

Receptor Information

>7bl5 Chain e (length=131) Species: 511145 (Escherichia coli str. K-12 substr. MG1655)

MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGV
YMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFA
KANAKFEVKAAAFEGELIPASQIDRLATLPT

Ligand information

>7bl5 Chain A (length=2913)

guuguaagguuaagcgacuaagcguacacgguggaugcccuggcagucag
aggcgaugaaggacgugcuaaucugcgauaagcgucgguaaggugauaug
aaccguuauaaccggcgauuuccgaauggggaaacccaguguguuucgac
acacuaucauuaacugaauccauagguuaaugaggcgaaccgggggaacu
gaaacaucuaaguaccccgaggaaaagaaaucaaccgagauucccccagu
agcggcgagcgaacggggagcagcccagagccugaaucaguguguguguu
aguggaagcgucuggaaaggcgcgcgauacagggugacagccccguacac
aaaaaugcacaugcugugagcucgaugaguagggcgggacacgugguauc
cugucugaauauggggggaccauccuccaaggcuaaauacuccugacuga
ccgauagugaaccaguaccgugagggaaaggcgaaaagaaccccggcgag
gggagugaaaaagaaccugaaaccguguacguacaagcagugggagcacg
cuuaggcgugugacugcguaccuuuuguauaaugggucagcgacuuauau
ucuguagcaagguuaaccgaauaggggagccgaagggaaaccgagucuua
acugggcguuaaguugcaggguauagacccgaaacccggugaucuagcca
ugggcagguugaagguuggguaacacuaacuggaggaccgaaccgacuaa
uguugaaaaauuagcggaugacuuguggcugggggugaaaggccaaucaa
accgggagauagcugguucuccccgaaagcuauuuagguagcgccucgug
aauucaucuccggggguagagcacuguuucggcaagggggucacuuacca
acccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacg
gcgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcu
aaggucccaaagucaugguuaagugggaaacgaugugggaaggcccagac
agccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauag
cucacuggucgagucggccugcgcggaagauguaacggggcuaaaccaug
caccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguu
cuguaagccugcgaaggugugcugugaggcaugcuggagguaucagaagu
gcgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccg
gaagaccaaggguuccuguccaacguuaaucggggcagggugagucgacc
ccuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauucc
uguacuugguguuacugcgaaggggggacggagaaggcuauguuggccgg
gcgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaaucc
ggaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaa
caaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaau
cguaccccaaaccgacacagguggucagguagagaauaccaaggcgcuug
agagaacucgggugaaggaacuaggcaaaauggugccguaacuucgggag
aaggcacgcugauauguaggugaggucccucgcggauggagcugaaauca
gucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugug
caaacacgaaaguggacguauacggugugacgccugcccggugccggaag
guuaauugaugggguuagcgcaagcgaagcucuugaucgaagccccggua
aacggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgg
guaaguuccgaccugcacgaauggcguaaugauggccaggcugucuccac
ccgagacucagugaaauugaacucgcugugaagaugcaguguacccgcgg
caagacggaaagaccccgugaaccuuuacuauagcuugacacugaacauu
gagccuugauguguaggauaggugggaggcuuugaaguguggacgccagu
cugcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaa
cguugacccguaauccggguugcggacagugucugguggguaguuugacu
ggggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaau
ccuggucggacaucaggagguuagugcaauggcauaagccagcuugacug
cgagcgugacggcgcgagcaggugcgaaagcaggucauagugauccggug
guucugaauggaagggccaucgcucaacggauaaaagguacuccggggau
aacaggcugauaccgcccaagaguucauaucgacggcgguguuuggcacc
ucgaugucggcucaucacauccuggggcugaaguaggucccaaggguaug
gcuguucgccauuuaaagugguacgcgagcuggguuuagaacgucgugag
acaguucggucccuaucugccgugggcgcuggagaacugaggggggcugc
uccuaguacgagaggaccggaguggacgcaucacugguguucggguuguc
augccaauggcacugcccgguagcuaaaugcggaagagauaagugcugaa
agcaucuaagcacgaaacuugccccgagaugaguucucccugacccuuua
aggguccugaaggaacguugaagacgacgacguugauaggccgggugugu
aagcgcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuua
accuuacaacgcc

<<<<<<<<<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<
[[<<......<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..]
.>>>......>>>>>>>...>>>....<<<....>>><<<<<<<....>>
>>>>>.<<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....
<<....>>......>>>>>.>.........<<....>>..<<<<<<....
.<<.....>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<
.<<<...<<<<<......>>>>>((......<<<.....))>>>...>>>
...>>>>>>>>>>>>>...>>>>........<<<<<<<<.........>>
>>>>>>......<<<<<..<<<<>>>>>..)))......]].>>>>>>>.
]]...............<<....>>....]].........<<<<.....>
>>>.............}....>>>>>>>>>>>.......<<<<..<<<<<
......>>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<
<<<<<<<<..<<<...<<.....>>...>>>...<<....>>..<<<<..
...>>>>.....>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<
<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<
<........>>>>>>>>....>>>>>>>>>...<<<.....>>>......
>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<
<...<<<<<<<<<.....<<<...<<<.<<<<...<<<<<<.>>>>.>>.
..>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<
<<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<.
..<<<(((.....<<<<<<...........<<..<<<<<..<<.......
.<<<<<......<<<<.......>>>>.............<<<......>
>>..>>>>>....>>..>>>>>.>>...........))).....>>>>>>
.>>>...>>>>>>><<<<<<.<<....>>.>>>>>>....>>>>>>..<<
<<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>..
......>>>>>><......<<.....<<<<<<.......>>>>>><<<<.
...<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<
<<...<<<...<<<....>>>...>>>...>>>..<<<<<........>>
>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<
<....(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>
>>>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>
>>...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>
>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<
<<....<<<<<<<<.<<...............<<<<<.............
..>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>
>>............<<<<<<<<<..<<<.........>>>..<<<<<<<.
....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<
<<....<...<<<<<<.<<....>>..>>>>>>..>.....>>>>>>>>.
.>>>>><<<<<.........>>>>>.....................<.<<
<.....>>>.>...........>>>>>..>>.>>>>>>>.....>>.>>>
>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<
<.(((.[[......<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<<<<
<<<<.<<<<...........<<<<<...<<......<<<<<<<<....>>
>>>>>>...>>.............>>>>>..>>>>.>>>>>>>>>>>...
<<<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<<
.....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<
<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<
<..<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<
<<.((..>>>>..))>>>>>>>.............>>>>..]]>>>>...
....<<<<....<<<<<...<<.......>>...>>>>>.....>>>.>.
......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<
<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>
>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<...<<<
<<<...............>>>>>>..<<<<<...<<<<...<<<<<<<<<
<<))).>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<...
........>>>>>....>>>.>>>>.......>>>>>>>..<<<<<....
.>>>>><....<<..<<<<<.......>>>>>....<<<<<<<<..<<..
..<<<<<....>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>
>>>>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bl5 Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): M1 K8 I11 S30 R31 G32 T34 K37 L41 V50 M52 R53 V54 V55 R56 T58 L59 R61 R62 E65 L81
• Binding residue (residue number reindexed from 1): M1 K8 I11 S30 R31 G32 T34 K37 L41 V50 M52 R53 V54 V55 R56 T58 L59 R61 R62 E65 L81

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0043022 ribosome binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006417 regulation of translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7bl5, PDBe:7bl5, PDBj:7bl5
• PDBsum: 7bl5
• PubMed: 33639093
• UniProt: P0A7J3|RL10_ECOLI Large ribosomal subunit protein uL10 (Gene Name=rplJ)