Structure of PDB 6q95 Chain e Binding Site BS01

Receptor Information

>6q95 Chain e (length=63) Species: 300852 (Thermus thermophilus HB8)

PKMKTHKGAKKRVKITASGKVVAMKTGKRHLNWQKSGKEIRQKGRKFVLA
KPEAERIKLLLPY

Ligand information

>6q95 Chain 1 (length=2831)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
gcgcggggggaaccuggggaacugaaacaucucaguacccagaggagagg
aaagagaaaucgacucccugaguagcggcgagcgaaaggggaccagccua
aaccguccggcuuguccgggcggggucgggggcccucggccgaaucccca
gccuagccgaagcuguugggaagcagcgccagagagggugaaagccccgu
aggcgaaagguggggggauaggugaggguacccgaguaccccgugguucg
uggagccaugggggaaucugggcggaccaccgccuaaggcuaaguacucc
gggugaccgauagcgcaccaguaccgugagggaaaggugaaaagaacccc
gggaggggagugaaauagagccugaaaccgugggcuuacaagcagucacg
gccccaagggguuguggcgugccuauugaagcaugagccggcgacucacg
gucgugggcgagcuuaagccguugaggcggaggcguagggaaaccgaguc
cgaacagggcgcguccgcggccguggacccgaaaccgggcgagcuagccc
uggccagggugaagcuggggugagacccaguggaggcccgaaccgguggg
ggaugcaaaccccucggaugagcuggggcuaggagugaaaagcuaaccga
gcccggagauagcugguucuccccgaaaugacuuuagggucagccucagg
cgcugacuggggccuguagagcacugauagggcuagggggcgccuaccaa
acccugucaaacuccgaagggucccagguggagccugggagugagggcgc
gagcgauaacguccgcguccgagcgcgggaacaaccgagaccgccagcua
aggcccccaagucugggcuaagugguaaaggauguggcgccgcgaagaca
gccaggagguuggcuuagaagcagccauccuuuaaagagugcguaauagc
ucacuggucgaguggcgccgcgccgaaaaugaucggggcuuaagcccagc
gccgaagcugcgggucugggggaugaccccaggcgguaggggagcguucc
cgaugccgaugaaggccgacccgcgagggcggcuggagguaagggaagug
cgaaugccggcaugaguaacgauaaagagggugagaaucccucucgccgu
aagcccaaggguuccuacgcaauggucgucagcguaggguuaggcgggac
cuaaggugaagccgaaaggcguagccgaagggcagccgguuaauauuccg
gcccuucccgcaggugcgauggggggacgcucuaggcuagggggaccgga
gccauggacgagcccggccagaagcgcagggugggagguaggcaaauccg
ccucccaacaagcucugcgugguggggaagcccguacgggugacaacccc
ccgaagccagggagccaagaaaagccucuaagcacaaccugcgggaaccc
guaccgcaaaccgacacaggugggcgggugcaagagcacucaggcgcgcg
ggagaacccucgccaaggaacucugcaaguuggccccguaacuucgggag
aaggggugcucccuggggugaugagccccggggagccgcagugaacaggc
ucuggcgacuguuuaccaaaaacacagcucucugcgaacucguaagagga
gguauagggagcgacgcuugcccggugccggaaggucaaggggaggggug
caagccccgaaccgaagccccggugaacggcggccguaacuauaacgguc
cuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaaaagc
guaacgaccggagcgcugucucggcgagggacccggugaaauugaacugg
ccgugaagaugcggccuacccguggcaggacgaaaagaccccguggagcu
uuacugcagccugguguuggcucuuggucgcgccugcguaggauaggugg
gagccuggcgccggugaaauccacccuggcgcggcugggggccuaacccu
cggauggggggacagcgcuuggcgggcaguuugacuggggcggucgccuc
cuaaaagguaacggaggcgcccaaagguccccucaggcgggacggaaauc
cgccggagagcgcaaggguagaagggggccugacugcgaggccugcaagc
cgagcaggggcgaaagccgggccuagugaaccgguggucccguguggaag
ggccaucgaucaacggauaaaaguuaccccggggauaacaggcugaucuc
ccccgagcguccacagcggcggggagguuuggcaccucgaugucggcucg
ucgcauccuggggcugaagaaggucccaaggguugggcuguucgcccauu
aaagcggcacgcgagcuggguucagaacgucgugagacaguucggucucu
auccgccacgggcgcaggaggcuugaggggggcucuuccuaguacgagag
gaccggaagggacgcaccucugguuucccagcugucccuccaggggcaua
agcuggguagccaugugcggaagggauaaccgcugaaagcaucuaagcgg
gaagcccgccccaagaugaggccucccacggcgucaagccgguaaggacc
cgggaagaccacccgguggaugggccggggguguaagcgccgcgaggcgu
ugagccgaccggucccaaucguccgaggucu

.........<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<<
<<<.....((.>>>.....<<<<<<<..<<<)).....>>>.....>>>>
>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<<<.
.>>>>>.>>...<<<<<<.....<<....>>......>>>>>.>......
...<<....>>...<<<<<.....<<.....>>....>>>>>...<<<..
..<<<<<.<<.....>>.>>>>>.....<<<.<<<.....<...<<<<<.
<<<<.<<<...<<<<<......>>>>>((......<<<.....))>>>..
.>>>...>>>>..>>>>>..>....>>>..>>>.....<<<<<<<<<...
....>>>>>>>>>......<<<<<..((((>>>>>..>>>......>>.>
>>>>>.>>...............<<....>>....>>.........<<<<
....>>>>..................>>>>>>>>>>>.......<<<<<<
<<<<....>>>>>>>>>>...<.<........>.>.<<<<<<....<<<<
<<<<<<<<...<<<...<<<.....>>>...>>>...<<....>>..<<<
<.....>>>>...>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<
<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<..<<<
<........>>>>.>>>....>>>>>>>>>...<<<.....>>>......
>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<
<.....<<<<<<<<<....<<...<<<<<<<<.....<......>.....
.>>>>>>>>..>>.....>>>>>>>>>.....>>>>>>>.>>..<<<<<<
<.<<......>>.>>>>>>>.....<<<......>>>...<<<<.<<<..
.<<<(((.....<<<<<<...........<<..<<<<<<<<<........
<<.<<.<<<..<<<.........>>>..>>>...................
...>>.>>....>>>>>>>>>.>>..........)))......>>>>>>.
>>>...>>>>>>><<<<<<<.......>>>>>>>....>>>>>>..<<<<
<...<<<......<<<<<.<<<....>>>>>>>>...>>>..>>>>>...
.....>>>>>><......<<.....<<<<<<.......>>>>>><<<<..
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<.<
<...<<<...<<<....>>>...>>>...>>...<<<<<........>>>
>>))<<<<<<<<<<<<<...<<<<....<<<<<.<<<..<<<<<.<<<<.
....[[[[.....>>>><<<...<<<<<<<<<..<<<<<.<<.....>>>
>>>>.......>>>>>>>>>..>>><...<<<<....>>>>.....>>>>
>>...>>>..>>>>>.........>>>>...>>...>>>>>>>>>>>>>>
>..>>.....<<......>>>>>>>.<<<<<....>>>>>..>>>>..<<
<<....<<<<<<<<.<<.........<.....<<<<<.....<..>....
..>>>>><<<<<<<<<<<<.....>>>>>>>>>>>>............<<
<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>>..
.....>>>>>>>.<<<<<.<<<..<<<<<<<..<<<...<<...<<<<<.
...>>>>>...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>
........<...........<<<<<<.....>>>>>>.....>....>>>
>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<
<<<........>>>>>..<<<<<<<<.<<<.........<<<<.<<<<<<
..<<<<<.<<<.<<<<<.<<<<<<<<.<<<<<.............<<<<<
....................>>>>>....>>>>>.>>>>>>>>...<<<<
<....>>>>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<
<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>>
>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<......>>
>..>>>>.<<<....>>>.>>))).......<<<<<<<.<<..))))..>
>>>>>>>>.............>>>>..>>>>>>.......<<<<...<<<
<<<...<<.......>>...>>>>>>....>>>.>.......<<<<<.<<
<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>..
...>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.......
.>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<...........
....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<]]]]>>>>>>..
.>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>>>
....>>>.>>>>.......>>>>>>>...................<<<<<
<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>
...>>>..>>>>>.>>>...>>>>.......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6q95 How a circularized tmRNA moves through the ribosome.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): P2 K3 M4 K5 T6 H7 K8 K12 R13 T17 A18 S19 K21 K26 T27 G28 K29 R30 H31 L32 N33 W34 Q35 I41 R42 Q43 R46 K47 F48 K52 E54 L61 L62 Y64
• Binding residue (residue number reindexed from 1): P1 K2 M3 K4 T5 H6 K7 K11 R12 T16 A17 S18 K20 K25 T26 G27 K28 R29 H30 L31 N32 W33 Q34 I40 R41 Q42 R45 K46 F47 K51 E53 L60 L61 Y63

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6q95, PDBe:6q95, PDBj:6q95
• PDBsum: 6q95
• PubMed: 30765567
• UniProt: Q5SKU1|RL35_THET8 Large ribosomal subunit protein bL35 (Gene Name=rpmI)