Structure of PDB 5t5h Chain e Binding Site BS01

Receptor Information

>5t5h Chain e (length=245) Species: 5693 (Trypanosoma cruzi)

GKTVLTCRKGNGSIYQVHGHKRLGPAKLRILDYAERHGFMRGVVKTIEHE
PGRGAPLARVEFRHPYKYRRVKELMVAPEGMFTGQSVLCGVKAPLAIGNV
LPLGQITEGCIVCNVEAKVGDRGTIARASGDYCIIISHNHETGRTRLKLP
SGQKKTVPSNCRAMIGIIAGGGRIEKPVLKAGNSFYRFRGKRNCWPKVRG
VARNPVEHPHGGGNHQHIGHPSTVSRHAPPGQKVGLIAARRTGRI

Ligand information

>5t5h Chain A (length=1278)

cagaccugaguguggcaggacuacccgccgaacuuaagcauauuacuaag
cggaggaaaagaaaacaaccgugauucucucagucagcggcgagcgaaga
gggauccaacucguugccgaaucgguucuuauaggaacuugaguuguggc
aauggugcaggaauggugaaauucaaagcaaacccguugcuacaucaugu
gaguauugagccaaagaagguguuagcccauugagccaugaaccuaagag
aguagcacugcuugggagugcagugucaagcuggcagguaugugucugcc
aaaguuaaauacagaguaggaagaccgauagcgaacaaguagcgugagcg
aaaguuugaaaagcacuuuggaaagagagugacauagaccugaagucgug
acaauaauggaaguaccuccauuucgccaaaaccagccucuacugagcaa
gaacgaggucaugguuuuggcggaaauggggugcccccacccgucuugaa
acacggaccaaggagucaaacagacgcgcaagggagaagauaucaguacu
ucucucguacugaaagggagauuguggugugcacacuugcccaacaccga
ccggccccggguugaguuggagugugccuguuuggacccgaaagguggug
aacuaugccugaacaaggugaagcccggggaaacucggguggaggccugu
agcgaugcugacgugcaaaucgcucgcaugauuuggguaucggagcgaaa
gacucaucgaaccaccuaguagcugguucacaucgaaguuucccucagga
uagcuggugcuugaaaaaguauugugcgguaaagcuuuaaaugugcaacu
ugccaaagcaagguauuaaggcaccaagugggccuucuucgguaagcgga
gcaggcgaugcggaaugaaccgcuuagggauauguggcgucaaaacuuau
gggcucacgaacaauggaugacgcucaagcccaauuagugcccauuauuc
cuuugguaagggcgagaaaguguagcccuuccgauguagauguggcgugg
agguaaggacgaagcugauggcgugagccucagauggaccagccucuagu
gcagaucuugguuggcguagcaaagaucuaacggaacguuggauacugga
gcggggaaggauuucgugccaacggcacucguacacgaguuguucggaua
cugagcacaacguuacaccguuuuuaggaaaaggggcaacagagaaccug
ggauuauauuccaaaaagaaauugcaug

.......................<<<<<......<......>.......>
>>>.>.........<<....>>...<<<<<......<<.....>>....>
>>>>....<<<..<<<<<<..<<<<<<<......>>>>>.>>....>>>>
>>......<<<.<<<<<......<<<<<<<.......>>>>.<.....>.
.....>>>.<<.......((.....>>)).....>>>>>...>>>.....
....<<<<<<<........>>>>>>>......<<<<<........>>>>>
..>>>....................................<<....>>.
...............<<<......>>>.......................
.......<<<..<<<<<<.<<<<<.<<<<<<<<<<<<<<...<<.>>...
.....>>>>..>>>>>>>>>>..>>>>>>>>>>>..>>>.<<<<......
..>>>><<<<<<...<<<<<<<<.<<<<<..<<<<..<<.<<<..>>>>>
..>>>>...<<....>>........<<<<<...<<..>>....>>>>><<
<<<<<<<.>>>>>>.>>>...>>>>>.>>>>>>>>.<<.<...<<<<<<.
....<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>..
.<<<<.<<.<<........>>>>>>>>....>>>>>>>>>...<<.....
..>>......>>>>>>......>.>>...<<<<<....<.<<<....>>>
(><<<<<<<<<.....<<...<<<<<<<...<<<.>>>...>>>>>>><<
<<<....>>>>>...>>..>>>>>>>.>>).<<<<.<<<<<.....>>>>
>.>>>>...<<<<......>>>>..<<<<<<<...<<<(((......<..
<<<<....<..........))).>....>>>>.>.....>>>...>>>>>
>><<<<.<<<<<.............>>>>>>>>>....>>>>>.....<<
<<<<..........<<<..<<<....>>>.>>>........>>>>>>...
......>>>>>>..............<<<<<<<...>>>>>>>.......
.............<<<<<....<<.....>>..>>>>>....<<......
....<<.<<<<<......>>>>...........>.>>..........<<<
<.........>>....>>.>>.......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5t5h Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.
• Resolution: 2.54 Å
• Binding residue (original residue number in PDB): G2 K3 T4 R9 S14 I15 Y16 H19 H21 K181 A182 G183 F186 Y187 N194 W196 V199 A203 R204 N205 V207 E208
• Binding residue (residue number reindexed from 1): G1 K2 T3 R8 S13 I14 Y15 H18 H20 K180 A181 G182 F185 Y186 N193 W195 V198 A202 R203 N204 V206 E207

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5t5h, PDBe:5t5h, PDBj:5t5h
• PDBsum: 5t5h
• PubMed: 27791004
• UniProt: Q4DPQ8