Structure of PDB 8wif Chain d Binding Site BS01

Receptor Information

>8wif Chain d (length=207) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

GQKINPHGFRLGITTEWKSRWYADKQYKDYVKEDVAIRKLLATGLERAGI
ADVEIERTRDRVRVDIHTARPGIVIGRRGTEADRIRADLEKLTGKQVQLN
ILEVKNPESQAQLVAQGVAEQLSNRVAFRRAMRKAIQSAMRQPNVKGIRV
QCSGRLGGAEMSRSEFYREGRVPLHTLRADIDYGLYEAKTTFGRIGVKVW
IYKGDIV

Ligand information

>8wif Chain a (length=1517)

uuuggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaac
acaugcaagucgaacggaaaggcccuuucggggguacucgaguggcgaac
gggugaguaacacgugggugaucugcccugcacuuugggauaagccuggg
aaacugggucuaauaccgaauacacccugcuggucgcauggccugguagg
ggaaagcuuuugcggugugggaugggcccgcggccuaucagcuuguuggu
ggggugauggccuaccaaggcgacgacggguagccggccugagaggguga
ccggccacacugggacugagauacggcccagacuccuacgggaggcagca
guggggaauauugcacaaugggcgcaagccugaugcagcgacgccgcgug
agggaugacggccuucggguuguaaaccucuuucagcacagacgaagcgc
aagugacgguaugugcagaagaaggaccggccaacuacgugccagcagcc
gcgguaauacguaggguccgagcguuguccggaauuacugggcguaaaga
gcucguaggugguuugucgcguuguucgugaaaacucacagcuuaacugu
gggcgugcgggcgauacgggcagacuagaguacugcaggggagacuggaa
uuccugguguagcgguggaaugcgcagauaucaggaggaacaccgguggc
gaaggcgggucucugggcaguaacugacgcugaggagcgaaagcgugggg
agcgaacaggauuagauacccugguaguccacgccguaaacgguggguac
uagguguggguuuccuuccuugggauccgugccguagcuaacgcauuaag
uaccccgccuggggaguacggccgcaaggcuaaaacucaaaggaauugac
gggggcccgcacaagcggcggagcauguggauuaauucgaugcaacgcga
agaaccuuaccuggguuugacaugcacaggacgccggcagagaugucggu
ucccuuguggccugugugcagguggugcauggcugucgucagcucguguc
gugagauguuggguuaagucccgcaacgagcgcaacccuugucucauguu
gccagcacguuaugguggggacucgugagagacugccggggucaacucgg
aggaagguggggaugacgucaagucaucaugccccuuauguccagggcuu
cacacaugcuacaauggccgguacaaagggcugcgaugccgugaggugga
gcgaauccuuucaaagccggucucaguucggaucggggucugcaacucga
ccccgugaagucggagucgcuaguaaucgcagaucagcaacgcugcggug
aauacguucccgggccuuguacacaccgcccgucacgucaugaaagucgg
uaacacccgaagccgguggccuaacccuuguggagggagccgucgaaggu
gggaucggcgauugggacgaagucguaacaagguagccguaccggaaggu
gcggcuggaucaccucc

......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<
<<.<<<..<<<..<<.<..<<<<<<<....>>>>>.>>>..>>>>>....
..<<........<<<<<<<..<<...<<<<<<<<<<<<.....<<<<<<.
...>>>>>>......>>>>.....<<<<<<<<<<<<..>>>>>.>>>>>>
>....<<....>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<
<<<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>..
.>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>
>>>.........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<
<<<......<<<<....>>>>.....>>>>...<.<<<<<......<<..
..>>.......>>>>>....>..<<<<<(((...<<<<<.....<<.)))
>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((.....
<<<<...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<<.....>>>>
>>>...>>>>>>>..>>>>>>>>>>...<<<<<<<<...<<<<<<<....
<<<<<<<<<..<<<......>>>.....>>>>>>>>>...........<<
....>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<..
.<<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<
..<<<<<<<<<<<<.......>>>>>>>>>>>>...<<..))>>.....>
>>>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<
<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>.
.........<<<<<<<......<<<<<<<...<<<<<<<....>>>>>>>
.<<.....>>.>>>>>>>.<<<.<<<..<<<<<<<......<<<<<<<<<
....>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<...
.<<..<<<......>>>>>.....>>>>>>.....<<<<<.....>>>>>
........>>>>.......>.>>>...>>>>>>>>>...>>>>>>>...>
>.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..
>>....>>>......>>>>>>>......<....<<<<<<<........>>
>>>>>....>.....>>>>>>....<<<<<<<..........>>>>>>>.
.....>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<
<..<<<<....<.<<<<..<<<..<<.....>>.>>>.>>>>.>...>>>
>..>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>
>>>>>>...........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8wif Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): G2 Q3 K4 I5 F10 K26 R150 S154 R156 E161 M162 S163 R169 R172 V173 P174 H176 T177 L178 R179 E188 T192 F193 R195 K199
• Binding residue (residue number reindexed from 1): G1 Q2 K3 I4 F9 K25 R149 S153 R155 E160 M161 S162 R168 R171 V172 P173 H175 T176 L177 R178 E187 T191 F192 R194 K198

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8wif, PDBe:8wif, PDBj:8wif
• PDBsum: 8wif
• PubMed: 38245551
• UniProt: A0QSD7|RS3_MYCS2 Small ribosomal subunit protein uS3 (Gene Name=rpsC)