Structure of PDB 8oz0 Chain d Binding Site BS01

Receptor Information

>8oz0 Chain d (length=198) Species: 9606 (Homo sapiens)

GISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGG
NKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCI
VLIDSTPYRQWYESHYALPLGRKEEEILNKKRSKKIQKKYDERKKNAKIS
SLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKG

Ligand information

>8oz0 Chain W (length=1716)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucugaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucgccuacuuggauaacuguggu
aauucuagagcuaauacaugccgacgggcgcugacccccuucgcgggggg
gaugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgca
cgccccccguggcggcgacgacccauucgaacgucugcccuaucaacuuu
cgaugguagucgccgugccuaccauggugaccacgggugacggggaauca
ggguucgauuccggagagggagccugagaaacggcuaccacauccaagga
aggcagcaggcgcgcaaauuacccacucccgacccggggagguagugacg
aaaaauaacaauacaggacucuuucgaggcccuguaauuggaaugagucc
acuuuaaauccuuuaacgaggauccauuggagggcaagucuggugccagc
agccgcgguaauuccagcuccaauagcguauauuaaaguugcugcaguua
aaaagcucguaguuggaucuugggagcggccccgccccucucggcggccc
gaagcguuuacuuugaaaaaauuagaguguucaaagcaggcccgagccgc
cuggauaccgcagcuaggaauaauggaauaggaccgcgguucuauuuugu
ugguuuucggaacugaggccaugauuaagagggacggccgggggcauucg
uauugcgccgcuagaggugaaauucuuggaccggcgcaagacggaccaga
gcgaaagcauuugccaagaauguuuucauuaaucaagaacgaaagucgga
gguucgaagacgaucagauaccgucguaguuccgaccauaaacgaugccg
accggcgaugcggcggcguuauucccaugacccgccgggcagcuuccggg
aaaccaaagucuuuggguuccggggggaguaugguugcaaagcugaaacu
uaaaggaauugacggaagggcaccaccaggaguggagccugcggcuuaau
uugacucaacacgggaaaccucacccggcccggacacggacaggauugac
agauugauagcucuuucucgauuccgugggugguggugcauggccguucu
uaguugguggagcgauuugucugguuaauuccgauaacgaacgagacucu
ggcaugcuaacuaguuacgcgacccccgagcggucggcgucccccaacuu
cuuaagggacaaguggcguucagccacccgagauugagcaauaacagguc
ugugaugcccuuagauguccggggcugcacgcgcgcuacacugacuggcu
cagcgugugccuacccuacgccggcaggcgcggguaacccguugaacccc
auucgugauggggaucggggauugcaauuauuccccaugaacgaggaauu
cccaguaagugcgggucauaagcuugcguugauuaagucccugcccuuug
uacacaccgcccgucgcuacuaccgauuggaugguuuagugaggcccucg
gaucggccccgccgggggcccuggcggagcgcugagaagacggucgaacu
ugacuaucuagaggaaguaaaagucguaacaagguuuccguaggugaacc
ugcggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<...<....<.....<<..........>>....>.>.......<<...
.....<<<.<....<<...<.<<<<...........<<.....<<.<<..
.....>>.>>......>>.........<<<<...<<<<........>>>>
...>>>><<..<<<<<.............>>>>>......>>....<<<.
.<<<......>>>.>>>..>>>>.>...>>..>.>>>.<<<....<<<..
..<<<<<<<.........>>>>>>>>>>......>>>...<<<.<<<<..
..>>>>....>>>.>>.<<.<<<..........>>>.>>.<.<<....>>
.>...>>>>>>.........<<<....<<<<...>>>>..>>>..>...>
>.>.....<..<<<<<<............>>.>>>>...>......<<..
<...........>..>>.........<<<<<((....<.<<<<.....<<
..))>>.......>>>>>>>>>>..>>>>>>.>>>.........<.<((.
....<.<<...<<<.<<.....<<..........>>.<<...........
..>>...<<<<<<.<.......<<...<.......>.<<<<.......>>
>>...>>......>.>>>..>>>........<.<<.<<<..<<.......
........>>..>>>.>>.>....>>....<<<<<<..<...<<<<..<<
..<<<<<<<<...<<<......>>>......>>>>>>>>..>>.......
<<....>>...>>>>..>..>>>.>>>...>>>...>>.>....<<<<<<
<...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<.
<<.<<<..<.<<<<<<.<<<........>>>>>>>>>.>..>>>...<<.
.))>>...>>.....>>>.>.>.<<<......<<<<....>>>>....>>
>..)))).]<<<...<<<<<<<..<<..<<<<<..<<<.<<<<<<.....
.<<........>>..........<<<<<......<<<<<<.......<<.
<<<........>>>.>>.....>>>>>>...<<.<<<..<<.<<<<<<..
..<<<.<<<<......>>...<<<......>>>...>>.>>>....<<<.
......<<<<...<<<<<<<<<<<.......>>>>.>>>>.....>>>..
.>>>.>......<<<<<.....>>>>>........>>>....>>>.>>>.
....>>>>>>>.......>>>>>...>>.>>>>.>>>.....<<..<<..
......<.......<<<.<<<<....>>>>.>>>....>.........>>
..>>......<.....<<<<<..........>>>>>.....>.....>>>
>>.....<<<<<<<<.......>>>>>>>>......>>...>>.>>>>>.
>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<..<<<<.<<.
..<<<<<.......................>>>>>...>>.>>>>..>>>
.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<....>>
>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8oz0 The structure of a human translation initiation complex reveals two independent roles for the helicase eIF4A.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): G2 R5 N7 H9 K10 R12 T14 K19 Y21 K23 K24 R25 Y27 R31 A33 R42 I43 H44 T45 R47 R49 G50 K54 Y55 R56 L58 R59 N64 S66 C72 T73 R74 S86 K98 S142 I145 K148 Y149 R178 Q181 R184
• Binding residue (residue number reindexed from 1): G1 R4 N6 H8 K9 R11 T13 K18 Y20 K22 K23 R24 Y26 R30 A32 R41 I42 H43 T44 R46 R48 G49 K53 Y54 R55 L57 R58 N63 S65 C71 T72 R73 S85 K97 S133 I136 K139 Y140 R169 Q172 R175

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8oz0, PDBe:8oz0, PDBj:8oz0
• PDBsum: 8oz0
• PubMed: 38287194
• UniProt: P62241|RS8_HUMAN Small ribosomal subunit protein eS8 (Gene Name=RPS8)