Structure of PDB 7nwi Chain cc Binding Site BS01

Receptor Information

>7nwi Chain cc (length=61) Species: 9986 (Oryctolagus cuniculus)

PIKLARVTKVIGKTGSQGQCTQVRVEFMDDTSHSTIRNVKGPVREGDVLT
LLESEREARRL

Ligand information

>7nwi Chain 9 (length=1719)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgccacuuggauaacugugguaauuc
uagagcuaauacaugccgacgggcgcugacccccuucgcgggggggaugc
gugcauuuaucaguggugacucuagauaaccucgggccgaucgcacgccc
cccguggcggcgacgaccauucgaacgucugcccuaucaacuuucgaugg
uagucgccgugccuaccauggugaccacgggugacggggaaucaggguuc
gauuccggagagggagccugagaaacggcuaccacauccaaggaaggcag
caggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaau
aacaauacaggacucuuucgaggcccuguaauuggaaugaguccacuuua
aauccuuuaacgaggauccauuggagggcaagucuggugccagcagccgc
gguaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagc
ucguaguuggaucuugggagcgggcguccccgccgccucucggccggggc
ccgaagcguuuacuuugaaaaaauuagaguguucaaagcaggcccgagcc
gccuggauaccgcagcuaggaauaauggaauaggaccgcgguucuauuuu
guugguuuucggaacugaggccaugauuaagagggacggccgggggcauu
cguauugcgccgcuagaggugaaauucuuggaccggcgcaagacggacca
gagcgaaagcauuugccaagaauguuuucauuaaucaagaacgaaagucg
gagguucgaagacgaucagauaccgucguaguuccgaccauaaacgaugc
cgaccggcgaugcggcggcguuauucccaugacccgccgggcagcuuccg
ggaaaccaaagucuuuggguuccggggggaguaugguugcaaagcugaaa
cuuaaaggaauugacggaagggcaccaccaggaguggagccugcggcuua
auuugacucaacacgggaaaccucacccggcccggacacggacaggauug
acagauugauagcucuuucucgauuccgugggugguggugcauggccguu
cuuaguugguggagcgauuugucugguuaauuccgauaacgaacgagacu
cuggcaugcuaacuaguuacgcgacccccgagcggucggcgucccccaac
uucuuagagggacaaguggcguucagccacccgagauugagcaauaacag
gucugugaugcccuuagauguccggggcugcacgcgcgcuacacugacug
gcucagcgugugccuacccuacgccggcaggcgcggguaacccguugaac
cccauucgugauggggaucggggauugcaauuauuccccaugaacgagga
auucccaguaagugcgggucauaagcuugcguugauuaagucccugcccu
uuguacacaccgcccgucgcuacuaccgauuggaugguuuagugaggccc
ucggaucggccccgccggggugcccuggcggagcgcugagaagacggucg
aacuugacuaucuagaggaaguaaaagucguaacaagguuuccguaggug
aaccugcggaaggaucauu

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<...<<<..........>>>..>>.>>......<<..<
.....<<<.<....<<....<<<<.......<<.....<<.<<<.....>
>>.>>......>>.........<<<<...<<<<<<....>>>>>>...>>
>><...<<<<<...<......>..>>>>>........>..<<<<...<<.
.....>>..>>>>..>>>>...>>..>.>>>.<<<....<<<....<<<<
<<<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>
....>>>>>>.<<.<<<..........>>>.>>.<.<<....>>.>...>
>>>>>.........<<<....<<<<...>>>>..>>>..>...>>.>...
..<<.<<<<<<.<<<....>>>..>>>>>>..>>......<<..<.....
......>..>>.........<<<<<((....<.<<<<.....<<..))>>
.......>>>>>>>>>>..>>>>>>.>>>.........<.<((.....<.
<<...<<<.<<.....<<...<<....>>...>>.....<<<........
>.>>.....<<<<<<.<.......<<...<.......>.<<<<.......
>>>>...>>......>.>>>..>>>.......<<.<..<<<<<<......
..........>.>>>>>.>.>>....>>....<<<<<<......<<<<..
<<..<<<<<<<<...<<<......>>>......>>>>>>>.>.>>.....
..<<....>>...>>>>.....>>>.>>>...>>>...>>.>....<<<<
<<<...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<
<.<<.<<<....<<<<<<.<<<........>>>>>>>>>....>>>...<
<..))>>...>>.....>>>.>.>.<<<......<<<<....>>>>....
>>>..)))).]<<<<<.<<<<<<<..<<..<<<<<..<<<.<<<<<<...
...<<........>>..........<<<<<......<<.<<........<
<.<<<........>>>>>.......>>.>>...<<.<<<..<<.<<<<<<
....<<<.<<<<<....>>>...<<<......>>>...>>.>>>....<<
<...<...<<<....<<<....<<<...<<<....>>>..>>>....>>>
....>>>..>.....<<<<<.....>>>>>........>>>....>>>.>
>>.....>>>>>>>.......>>>>>...>>.>>>>.>>>.....<<.<<
<................<<<.<<<<....>>>>.>>>.............
.>>>.>>......<.....<<<<<..........>>>>>.....>.....
>>>>>.....<<<<<<<<.......>>>>>>>>......>>...>>>>>>
>>>>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<..<<<<.
<<...<<<<<.<<<<<<<<<..>>>>>>>>>...>>>>>...>>.>>>>.
.>>>.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<..
..>>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nwi Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.
• Resolution: 3.13 Å
• Binding residue (original residue number in PDB): S23 Q24 G25 P49
• Binding residue (residue number reindexed from 1): S16 Q17 G18 P42

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005791 rough endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nwi, PDBe:7nwi, PDBj:7nwi
• PDBsum: 7nwi
• PubMed: 34157102
• UniProt: G1TIB4|RS28_RABIT Small ribosomal subunit protein eS28 (Gene Name=RPS28)