Structure of PDB 6hcj Chain c3 Binding Site BS01

Receptor Information

>6hcj Chain c3 (length=98) Species: 9986 (Oryctolagus cuniculus)

SLESINSRLQLVMKSGKYVLGYKQSLKMIRQGKAKLVILANNCPALRKSE
IEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLSIIDPGDSDIIRS

Ligand information

>6hcj Chain 51 (length=3635)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcgcgguccggccgugccggcggggcgg
aucucccgcucccgucggcgggggaccgccccccggccggcgaccggccg
ccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggac
ggcugggaaggccccggcggggaagguggcccgggggccccggguguuac
agccccgccccggcagcagcagcucgccgaaucccggggccgagggagcg
agacccgugcgccgcgcucuccccccucgggggggccgggccaccccucc
cacggcgcgaccgcucucccacccggggggcggggcggacuguccccagu
gcgccccgggcgggucgcgccgucgggcccgggggagguucucucggggc
cacgcgucccccgcgagcgcacggggucggcggcgaugucggcuacccac
ccgacccgucuugaaacacggaccaaggagucuaacacgugcgcgagucg
ggggcuacgaaagccgccguggcgcaaugaaggugaaggccggcggcgcg
ccgccggccgaggugggaucccgaggccuuccaguccgccgagggcgcac
caccggcccgucucgcccgccgcgccggggagguggagcacgagcgcacg
uguuaggacccgaaagauggugaacuaugccugggcagggcgaagccaga
ggaaacucugguggagguccguagcgguccugacgugcgaaucggucguc
cgaccuggguauaggggcgaaagacuaaucgaaccaucuaguagcugguu
cccuccaaaguuucccucaggauagcuggcgcucuccgcaguuuuauccg
guaaagcgaaugauuagaggucuuggggccgaaacgaucucaaccuauuc
ucaaacuuuaaauggguaagaagcccggcucgcugguguggagccgggcg
uggaaugcgagugccuagugggccacuuuugguaagcagaacuggcgcug
cgggaugaaccgaacgccggguuaaggcgcccgaugccgacgcucaucag
accccagaaaagguguugguugauauagacagcaggaugguggccaugga
agucggaauccgcuaaggaguguguaacaacucaccugccgaaucaacua
gcccugaaaauggauggcgcuggagcgucgggcccauacccggccgucgc
uggcagucggcagagcgagaaagagaguggcgcggacgcuacgccgcgac
gaguaggagggccgcugcggugagccuugaagccuagggcgcgggcccgg
guggagccgccgcaggugcagaucuuggugguaguagcaaauauucaaac
gagaacuuugaaggccgaaguggagaaggguuccaugugaacagcaguug
aacaugggucagucgguccugagagaugggcgagcgccguuccgaaggga
cgggcgauggccuccguugcccucagccgaucgaaagggagucggguuca
gauccccgaauccggaguggcggagaugggcgccgcgaggcguccagugc
gguaacgcgaccgaucccggagaagccggcgggagccccggggagaguuc
ucuuuucuuugugaagggcagggcgcccuggaauggguucgccccgagag
aggggcccgugccuuggaaagcgucgcgguuccggcggcguccggugagc
ucucgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgg
gccguacccauauccgcagcaggucuccaaggugaacagccucuggcaug
uuggaacaauguagguaagggaagucggcaagccggauccguaacuucgg
gauaaggauuggcucuaagggcuggcagccgacuuagaacuggugcggac
caggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcgg
cggguguugacgcgaugugauuucugcccagugcucugaaugucaaagug
aagaaauucaaugaagcgcggguaaacggcgggaguaacuaugacucucu
uaagguagccaaaugccucgucaucuaauuagugacgcgcaugaauggau
gaacgagauucccacugucccuaccuacuauccagcgaaaccacagccaa
gggaacgggcuuggcggaaucagcggggaaagaagacccuguugagcuug
acucuagucuggcacggugaagagacaugagagguguagaauaaguggga
ggcccgggccgccggugaaauaccacuacucugaucguuuuuucacugac
ccggugaggcgggggggcgagccgggcucucgcuucuggcgccaagcgcc
cggccggccgggcgcgacccgcuccggggacagugccagguggggaguuu
gacuggggcgguacaccugucaaacgguaacgcagguguccuaaggcgag
cucagggaggacagaaaccucccguggagcagaagggcaaaagcucgcuu
gaucuugauuuucaguacgaauacagaccgugaaagcggggccucacgau
ccuucugaccuuuuggguuuuaagcaggaggugucagaaaaguuaccaca
gggauaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuug
auccuucgaugucggcucuuccuaucauugugaagcagaauucaccaagc
guuggauuguucacccacuaauagggaacgugagcuggguuuagaccguc
gugagacagguuaguuuuacccuacugaugauguguuguugccaugguaa
uccugcucaguacgagaggaaccgcagguucagacauuugguguaugugc
uuggcugaggagccaauggggcgaagcuaccaucugugggauuaugacug
aacgccucuaagucagaaucccgcccaggcggaacgauagcgccggagcc
ucgguuggccucggauagccggucccccgcccgggguccggugcggagag
cccuucgucccgggaaacggggcggccggaaagggggccgcccucucgcc
cgucacgcaccgcacguucguggggaaccuggcgcuaaaccauucguaga
cgaccugcuucugggucgggguuucguacguagcagagcagcugcugcga
ucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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..>>>.>..<<<.<<<<<....<<<..<<<..<<<<<<<<....<<<<..
....>>>>.............<<<.......>>>...>.>>>>>>>...>
>>..>>>...>>>>.>>>>.......<<<<<<<........>>>>>>>..
....<<<<..(((((.>>>>...>>>........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<..<....<<
<<<<<<.........<<<<<<<<<<....<<<<<<<.<<....<<<<<<<
.<<<<<<<<<......>>>>>>>>>>>>>>>>...>>>>>>...>>>.>>
>>>>>>>>..........>>>.>>>>>...<<<<<<<.<<<<<<<<<<.<
<<<.........<..<<<<<<.......<<<<.<<<.>>>.>>>>.....
...>>>>>>...>............>>>>...>>>>>>>>>>.....<<.
......>>.............<<<<<<..>>>>>>...<.<<.<<<<.<<
<<<<<...<<<<<<....<..<<...>>>.<<<<<<<...<<....>>..
.>>>>>>>.>>.>>>>...>>>>.>>>...>.>>>...........>>.>
..........................>>.>>>>>....>.>>...>>>>>
>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..<<<.
..<<<.......>>>.....>>>...<<....>>...<<<<<<<<<<..>
>>>>>>>>>..<<<<<...<<<..<<........[...>>..>>>....>
>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>
>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<...<<.......<<.
<.....>>>........>>....<<<..<..<<........>>.>.>>>.
...>>>>>>>>>...<<.......>>......>>>>>>...>..>.>>..
<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<<.
...<<<....<<<...<<<<..<<<.<<.........>.>>>>>>>.>..
>>>...>>>....>>>>>>...<<<<<<<<<.<......>>>>>>>>>>.
...>>>>>>>>>>>>.>>>..<<<<.<<<<<.....>>>>>.>>>>....
<<<......>>>...<<<<<<<...<<.(((.......<<<<<.....<<
..........<<<.<<<..<<<.....................<<.....
....>>...............<<......>>.............>>>..>
>>>>>..........))).>>...>>>>>...>>...>>>>>>><<<<<.
.<<<.....................]................>>>.>>>>
>....>>>>>>..<<<<<<<<..<<......<<<<.<<<<....>>>>>>
>>...>>.>>>>>>>.>......>>>>>><<<....<<.....<<<<<<.
......>>>>>>.<<......<<.<..<<...<<<<<<...<<.....>>
.>>>>>>....(.<<<<<<...<<....<<<....>>>............
.<<<<<.<<<...>>>.>>>>>........>>....>>>...<<<<....
....>>>>..)<<<<....<<<......<<<<<......>>>>>....<<
<....>>>......<<<<<<<...<<<<<<<<<<<<<<<<<<<<<<<..[
[[..>>>>>>.....<<<..<<<<.<<<<.....<<<.....>>>.....
>>>>>>>>...>>>......<<<<<<<.......>>>>>>>>>>>.>.>>
>>>>>>>>>>.........>>>>>>>................>>>.>>>>
.>>>..>>.....<<......>>.>.>>.<<<<.....>>>>...>>...
.<<<<.....<<<<<<..<<...............<<<<<..........
.....>>>>><<.<<<<<<<<<<<.>>>>>..>>>>>>.>>.........
...<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<....
.>>>>......>>>>>>>..<.<..<<<..<<<<<.....<<<......<
<<....>>>......>>>..>>>..>>>>><..<<.........>>...>
.......<...........<<<<<.......>>>>>.....>.....>..
>..>>.>>>>>>>.....>>..>>>>>>..>>>>>>...>>>....<<<<
<........>>>>>.....<<<<..<<<.<<......<<<<....<<<..
<<<<..<<<<<<<<..<<<.<<<<...<.<<<<..........<<<<<..
.<<<..>>>.............>>>>>.>>>>.>...>.>>.>>>>...<
<<<<.....<<<<.................................<<<<
<.<<..>>.>>>>>...>>>>..>>>>>.....>>>>>>>>...>>>>..
..<<.....>>...<<<<<<...........>>>>>>..<<...<<<<<<
<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>>>>>>.
..<<.<<<<<<.......>>>>.>>>>.<<<....>>>.>>)))].....
.<<<<<<..<<<...))))).>>>>>>>>>..........>>>.....>>
>>.......<<..<<.<<<<<<...<<.......>>...>>>>>>...>>
.>.>.........<<<.<.<<<...<...<<<<<......>>>>>.....
>.<<<.........>>>......>>>...>...>>>.......<<<<<<<
....>>>.>>>>....>>..>>>.>>>>......<.<<<<....<<<..<
.<<<<<.................>>>>>.><<<<....<<<<....<<..
<.<<<.]]]..>>>....>.>>...>>>>...>>>><<<.....<<<<..
...........>>>>.....>>>.>>>.....>>>>.>.<<<<<<<..<<
<.....<<.........<<<<<....<<<..>>>...>>>>>........
>>...<<<..<<<<....<<<.<<<<<........>>>>>..>>>...>>
>>..>>>...............>>>..>>.>>>>>.<<<<<.<.......
......>....<<.<<<<<<<.<<.....<<<<.<..<<<<<.>>>>>..
>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6hcj ZNF598 Is a Quality Control Sensor of Collided Ribosomes.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): L29 G30 K32 Q33 P53 A54 S58 Y88 Y89 R90 V91 C92
• Binding residue (residue number reindexed from 1): L20 G21 K23 Q24 P44 A45 S49 Y79 Y80 R81 V82 C83

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0031640 killing of cells of another organism
• GO:0050829 defense response to Gram-negative bacterium
• GO:0061844 antimicrobial humoral immune response mediated by antimicrobial peptide

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0014069 postsynaptic density
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6hcj, PDBe:6hcj, PDBj:6hcj
• PDBsum: 6hcj
• PubMed: 30293783
• UniProt: G1TDL2|RL30_RABIT Large ribosomal subunit protein eL30 (Gene Name=RPL30)