Structure of PDB 7sfr Chain c Binding Site BS01

Receptor Information

>7sfr Chain c (length=207) Species: 1773 (Mycobacterium tuberculosis)

GQKINPHGFRLGITTDWKSRWYADKQYAEYVKEDVAIRRLLSSGLERAGI
ADVEIERTRDRVRVDIHTARPGIVIGRRGTEADRIRADLEKLTGKQVQLN
ILEVKNPESQAQLVAQGVAEQLSNRVAFRRAMRKAIQSAMRQPNVKGIRV
QCSGRLGGAEMSRSEFYREGRVPLHTLRADIDYGLYEAKTTFGRIGVKVW
IYKGDIV

Ligand information

>7sfr Chain a (length=1519)

uggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaacac
augcaagucgaacggaaaggucucuucggagauacucgaguggcgaacgg
gugaguaacacgugggugaucugcccugcacuucgggauaagccugggaa
acugggucuaauaccggauaggaccacgggaugcaugucuugugguggaa
agcgcuuuagcggugugggaugagcccgcggccuaucagcuuguuggugg
ggugacggccuaccaaggcgacgacggguagccggccugagagggugucc
ggccacacugggacugagauacggcccagacuccuacgggaggcagcagu
ggggaauauugcacaaugggcgcaagccugaugcagcgacgccgcguggg
ggaugacggccuucggguuguaaaccucuuucaccaucgacgaagguccg
gguucucucggauugacgguagguggagaagaagcaccggccaacuacgu
gccagcagccgcgguaauacguagggugcgagcguuguccggaauuacug
ggcguaaagagcucguaggugguuugucgcguuguucgugaaaucucacg
gcuuaacugugagcgugcgggcgauacgggcagacuagaguacugcaggg
gagacuggaauuccugguguagcgguggaaugcgcagauaucaggaggaa
caccgguggcgaaggcgggucucugggcaguaacugacgcugaggagcga
aagcguggggagcgaacaggauuagauacccugguaguccacgccguaaa
cgguggguacuagguguggguuuccuuccuugggauccgugccguagcua
acgcauuaaguaccccgccuggggaguacggccgcaaggcuaaaacucaa
aggaauugacgggggcccgcacaagcggcggagcauguggauuaauucga
ugcaacgcgaagaaccuuaccuggguuugacaugcacaggacgcgucuag
agauaggcguucccuuguggccugugugcagguggugcauggcugucguc
agcucgugucgugagauguuggguuaagucccgcaacgagcgcaacccuu
gucucauguugccagcacguaaugguggggacucgugagagacugccggg
gucaacucggaggaagguggggaugacgucaagucaucaugccccuuaug
uccagggcuucacacaugcuacaauggccgguacaaagggcugcgaugcc
gcgagguuaagcgaauccuuaaaagccggucucaguucggaucggggucu
gcaacucgaccccgugaagucggagucgcuaguaaucgcagaucagcaac
gcugcggugaauacguucccgggccuuguacacaccgcccgucacgucau
gaaagucgguaacacccgaagccaguggccuaacccucgggagggagcug
ucgaaggugggaucggcgauugggacgaagucguaacaagguagccguac
cggaaggugcggcuggauc

....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<
.<<<..<<<..<<.<..<<.<<<......>>..>>>>..>>>>>......
<<........<<<<<<<..<<...<<<<<<<<<<<<..<..<<<<<....
..>>>>>.....>>>>>.....<<<<<<<<<<....>>>>>>>>>>....
.<<......>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<<<
<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>
>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>
>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<<
<......<<<<....>>>>.....>>>>...<.<<<<<......<<<<<<
.......>.>>>>>.......>>>>>....>..<<<<<(((...<<<<<.
....<..))).>.......>>>>>>>>>>..>>>>>>>>>..........
<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<
<.....>>>>>>>...>>>>>>>..>>>>>>>>>>...<<<<<<<<...<
<<<<<<....<<<<<<<<<..<<<......>>>.....>>>>>>>>>...
........<<....>>.>>>>>>>..>>>>>.>>>...>>>...>>>>..
..<<<<<<...<<...<<<<.<.....>.>>>>...>>>>>>>>......
....<<<<<<..<<<<<<<<<<.<........>>>>>>>>>>>...<<..
))>>.....>>>>>>.>>>.<<<......<<<<....>>>>....>>>..
)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<.
.......>>..........<<<<<<<.......<<<<<..<<<<.<.<..
..>.>.>>>>............>>>>>..<<<.<<<..<<<<<<......
.<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.....<<<<
.<<<<<<....<...<<<......>>>.>.....>>>>>>.....<<<<<
.....>>>>>........>>>>.........>>>...>>>>>>>>>...>
>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<<...<<<
....>>>...>>....>>>.....>>>>>>>......<....<<<<<<<.
.......>>>>>>>....>.....>>>>>>....<<<<<<<.........
.>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<
<<..<<<<<<..<<<<....<.<<<<..<<<..<<....>>.>>>.>>>>
.>...>>>>..>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<
<....>>>>>>>>>.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7sfr Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agents.
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): G2 Q3 K4 I5 F10 I14 S154 E161 S163 R169 R172 V173 H176 L178 R179 E188 F193 G194 R195 K199
• Binding residue (residue number reindexed from 1): G1 Q2 K3 I4 F9 I13 S153 E160 S162 R168 R171 V172 H175 L177 R178 E187 F192 G193 R194 K198

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7sfr, PDBe:7sfr, PDBj:7sfr
• PDBsum: 7sfr
• PubMed: 36827973
• UniProt: P9WH37|RS3_MYCTU Small ribosomal subunit protein uS3 (Gene Name=rpsC)