Structure of PDB 7ohr Chain c Binding Site BS01

Receptor Information

>7ohr Chain c (length=97) Species: 559292 (Saccharomyces cerevisiae S288C)

SINQKLALVIKSGKYTLGYKSTVKSLRQGKSKLIIIAANTPVLRKSELEY
YAMLSKTKVYYFQGGNNELGTAVGKLFRVGVVSILEAGDSDILTTLA

Ligand information

>7ohr Chain 1 (length=2373)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaacgagguuggggccucgcaagaggugcacaaucgaccgauccugagga
uggauuugguaagagcauagcuguugggacccgaaagauggugaacuaug
ccugaauagggugaagccagaggaaacucugguggagcucgguucgacgu
ucgaucgugaauuggguauagggcgaaagacaaccaucuaguagcugguu
ccugccgaaguuucccuaggauagcagaagcucguaucaguuuuaugagu
aagaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcgau
gcgggaugaaccgaacguagaguuaaggugccggaauacacgcucaucag
acacaaaagguguuaguucaucuagacagccggacgguggccauggaagu
cggaauccgcuaaggaguguguaacaacucaccggccgaaugaacuagcc
cugaaaauggauggcgcucaagcguguuaccuauacucuaccgucagggu
ugauaugaugcccugacgaguaggcaggcguggaggucagugacgaagcc
uagaccguaaggucgggucgaacggccucuagugcagaucuuggugguag
uagcaaauauucaaaugagaacuuugaagacugaaguggggaaagguucc
acgucaacagcaguuggacguggguuagucgauccuaagagauggggaag
cuccguuucaaaggccugauuuuaugcaggccaccaucgaaagggaaucc
gguuaagauuccggaaccuggauauggauucuucacgguaacguaacuga
auguggagacgucggcgcgagcccugggaggaguuaucuuuucuucuuaa
cagcuuaucaccccggaauugguuuauccggagauggggucuuauggcug
gaagaggccagcacugcuggcuccggugcgcuugugacggccgugaaaau
ccacaggaaggaauaguuuucaugccaggucguacugggucuccaaggug
aacagccucuaguugauagaauaauguagauaagggaagucggcuguccc
uaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauc
agcggggacccuguugagcuugacucuaguuugacauuauugucaggugg
ggaguaaaguuaccacagggauaauggcuuguggcagucaagcguucagc
gacauugcucggcucuuccuaucauaccgaagcagaauucgguaagcguu
ggauuguucacccacuaauagggaacguggcuggguagaccgucgugaga
cgguuaguuuuacccuacugaugaauguuaccgcaauaguaauugaacuu
aguacgagaggaacaguucauucggauaauugguuuuugcggcugucuga
ucaggcaugccgcgaagcuaccauccgcuggauuauggcugaacgccucu
aagucagaauccaugcuagaacgcggugauuucuuugcuccacacaauau
agauggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggc
uagcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauug
caaugucauuuugcguggggauaaaucauuuguauacgacuuagauguac
aacgggguauuguaagcaguagaguagccuugguuacgaucugcugagau
uaagccuuuguugucugauuugu

..........................<<<<<<.....<......>.....
.>>>>>.>...<.....<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......
>>>>.((......<<<.....))>>>....>>>>>>>>>..>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>....>............................
.<<....>>...............<<<<....>>>>..............
....................<<<<<.<<<<<......<<<<<<<.<<<<<
<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>....
...>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<
<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.....
......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<......
.>>>.>>>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<...<<<<<
<<<<....<<<<...<<<<<<<....>>>>>>>...>>>>...<<<....
.>>>........>>>>>>>>>...........>>>>>>>.....>.>>..
<<<<<<....<...........><<<<<<<<<....<<<.<<<<<<..>>
>>.>>>>>..>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>...
.<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<
.......<<<.<<<<<<<<<<.......<<<<<.<<<...<<........
.>>...>>>.........<<......>>...>>>>>...>>>>>>>>>>>
>>..........))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<
.........>>>>>>>>>....>>>>>>..<<<<<<<..<<......<<<
<.<<<<....>>>>>>>>...>>.>>>>>>>........>>>>>><<...
..<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<
<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<...
.>>>........<<<<<<........>>>>>>....>>....>>...<<<
<<........>>>>>))<<<<.<<<<<<<.....<<<....>>>...<..
.<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..(((..>>>>>><...
<<<<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>
....><<<<<<<...>>>>>>>>>>>...>>>>>>>.>>>>........>
>>>>>...>......>.>>.>>>>>>>>>>>>..>>...>>>>>.<<<<.
....>>>>..>>>>..<<<<.....<<<<<<<.<<............>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<
<<..<<..<<<<<.<..<<<<..<<<<.<<<<<<<<....>>>>>>>>..
>>>>..>>>>....>>>>>>............<<<<<....<<.....>>
....>>>>>.<<...<<<<<..<.<<<<<<<......>>>>>>>...>.<
<<.........>>>.....>>>>>......>>.....<<<<<<<....>>
>>>>>.........>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<.
..............>>>>>>.><<<<<...<<<<...<<<<<<.<<<<))
).>>>>..>>>>>>..>>>>..>>>>>.<<<<...<<<<<..........
.>>>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<
....<<..........<<<<<<.....>>>>>>......>>.......<<
<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>......
.......>>.>>>.>>>>>>..<<<<<.<.............>.....<<
<<<.<<<.<<<....<<<<....<..<<<..>>>..>...>>>>...>>>
....>>>..>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohr Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 4.72 Å
• Binding residue (original residue number in PDB): L25 G26 Y27 K28 S29 K32 R52 F85 R86 V87 G88
• Binding residue (residue number reindexed from 1): L17 G18 Y19 K20 S21 K24 R44 F77 R78 V79 G80

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0030627 pre-mRNA 5'-splice site binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0048025 negative regulation of mRNA splicing, via spliceosome

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohr, PDBe:7ohr, PDBj:7ohr
• PDBsum: 7ohr
• PubMed: 34813592
• UniProt: P14120|RL30_YEAST Large ribosomal subunit protein eL30 (Gene Name=RPL30)