Structure of PDB 7a5i Chain a3 Binding Site BS01

Receptor Information

>7a5i Chain a3 (length=82) Species: 9606 (Homo sapiens)

TYSPLPDDYNCNVELALTSDGRTIVCYHPSVDIPYEHTKPIPREEGPMIE
QLSKMFFTTKHRWYPHGRYHRCRKNLNPPKDR

Ligand information

>7a5i Chain A3 (length=1485)

gcuaaaccuagccccaaacccacuccaccuuacuaccagcaaccuuagcc
aaaccauuuacauaaaguauaggcgauagaaauuguggcgcaauagauau
aguaccgcaagggaaagaugaaaaauuauaaccaagcauaauauagcaag
gacuaaccccuauaccuucugcauaaugaauuaacuagaaauaacuuugc
aaggagagccaaagcuaagacccccgaaaccagacgagcuaccuaagaac
agcuaagagcacacccgucuauguagcaaaauagugggaagauuuauagg
uagaggcgacaaaccuaccgagccuggugauagcugguuguccaagauag
aaucuuaguucaacuuuaaauuugcccacagaaccaaauccccuuguaaa
uuuaacuguuaguccaaagaggaacagcucuuuggacacuaggaaaaaac
cuuguagagagaguaaaaaauuuaacacccauaguaggccuaaaagcagc
caccaauuaagaaagcguucaagcucaacacccacuaccuaaaaaauccc
aaacauauaacugaacuccucacacccaauuggaccaaucuaucacccua
uagaagaacuaauguuaguauaaguaacaugaaaacauucuccuccgcau
aagccugcgucagaucacacugacaauuaacagcccaauaucuacaauca
accaacaagucauuauuacccucacugucaacccaacacaggcaugcuca
uaaggaaagguuaaaaaaaguaaaaggaacucggcaaaucuuaccccgcc
uguuuaccaaaaacaucaccucuagcaucaccaguauuagaggcaccgcc
ugcccagugacacauguuuaacggccgcgguacccuaaccgugcaaaggu
agcauaaucacuuguuccuuaaauagggaccuguaugaauggcuccacga
ggguucagcugucucuuacuuuuaaccagugaaauugaccugcccgugaa
gaggcgggcauaacacagcaagacgagaagacccuauggagcuuuaauuu
auuaaugcaaacaguaaccugcauuaaaaauuucgguuggggcgaccucg
gagcagaacccaaccuccgagcaguacaugcuaagacuucaccagucaaa
gcgaaccucaauugauccaauaacuugaccaacggaacaaguuacccuag
ggauaacagcgcaauccuauucuagaguccauaucaacaauaggguuuac
gaccucgauguuggaucaggacaucccgauggugcagccgcuauuaaagg
uucguuuguucaacgauuaaaguccuacgugaucugaguucagaccggag
uaauccaggucgguuucuaucuacuuuauuccucccuguacgaaaggaca
agagaaauaaggccuacuucacaaagcgccuucccccguaaaugauauca
ucucaacuuagauacccacacccaagaacaggguu

<<<.......<<<<....................................
<......>.....<.....<<.....>>....>...>>.>>.........
....<<....>>......................>>>...........<<
<.......>>>...<.<........>.>.<<<<<<.........<<<.<<
..<<....>>...>>.>>>...<<.<..<<<<<.<.....<<<<<.<<<.
.<<<...>>>...<<<..<<<<........>>>>.>>>....>>>.>>>>
>...<<.......>>......>.>>>>...>..>.>>..<<<<<<....<
<<<....>>>><<<.<<<<<<<<<<.....<<......>>.....>>>>>
>>>>>..>>>.<<<<<<<<<<......>>>>>>>>>>...<<<......>
>>....<<<.<<<...<..............<....<<<.........>>
>....>.......<<<......>>>.........................
...........>...>>>>>>........>>>>>>...<<<<<......>
>>>>.........>>>>>>.......<<..<<<....>>><<<<..<<<<
........<<<<<......>>>>>......<<<...<<<<..........
.............>>>>.......>>>.....<<......>>.>>>>...
..>>>>..<<<<...<<<<<<<<.<<................<<<<<<..
<<<.........>>>...<<<<<<.............>>>>>>....<<<
.<<<..<<<<<....>>>..>>>>><<<<<<......>>>>>>.......
.<...........<<<<<<.....>>>>>>.....>....>>>....>>.
>>>>........>>.>>>>>>>>>>>>.>>...........<<<<<....
....>>>>>.......<<..<<<.<<......<<<<.<<<<<<..<<<<<
.<<<<<<<<..........>>>>>>>>>>>>>..<<<<.....>>>><<<
<<<...........>>>>>>..<<.....<<<..<<<<.....>>>>..>
>>....>>.........<<<....>>>............>>>>..>>>>>
>.......<<<..<.<<<<<<...<<.......>>...>>>>>>...>.>
>.>.......<<<<<.<<<<<<..<<.<<<<<<......>>>>>>...>>
.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<.
...>>>.>>>>....>>..>>>.>>..<<<.<<<................
.>>>.>>><<<<<...<<<....>>>.>>>>>......<<<.........
.......>>>.........<<<<.......>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7a5i Elongational stalling activates mitoribosome-associated quality control.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): K120 H121 Y124 P125 H126 G127 R128 H130 R133 K134 P138
• Binding residue (residue number reindexed from 1): K60 H61 Y64 P65 H66 G67 R68 H70 R73 K74 P78

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0032543 mitochondrial translation

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005762 mitochondrial large ribosomal subunit
• GO:0005763 mitochondrial small ribosomal subunit
• GO:0005840 ribosome
• GO:0005886 plasma membrane
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7a5i, PDBe:7a5i, PDBj:7a5i
• PDBsum: 7a5i
• PubMed: 33243891
• UniProt: Q9Y6G3|RM42_HUMAN Large ribosomal subunit protein mL42 (Gene Name=MRPL42)