Structure of PDB 8bsi Chain a Binding Site BS01

Receptor Information
>8bsi Chain a (length=815) Species: 5741 (Giardia intestinalis) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
HFTTEQIRECMDHQDRIRNMSVIAHVDHGKSTLTDSLIAHAGGNTRFTDT
RQSGGYLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCAEGVCVQTETV
LRQALSERVIPCLMLNKVDRVIMELKLSGEDAFLMFEKTIGEVNQLIATY
QDKTLFNEKKYKFGNRTDLCVDPSRGNVAFGSGLHGWGFTVTHFARIYTK
KFGGELSTWMKNLWGNRFLNEKTGKWTGKSQGDNGEKNQRGFAIYVMDPI
LQLFDAVMTEQKKKYTKMLKQLNVTLTPDEEDMTGKRLLKAVMQKFLPAA
DALLEMIIVHLPSPKKAQQYRVDTLYTGPLDDPAAEAIRNCDPNGPLMLY
VSKMVPTVDKSRFFAFGRVFSGVVQTGQKVHIMGPEYHPGTSKKDELFIK
NIQRTILMMGSRIEQIDDVPCGNTVGLVGIDQYLVKSGTISTYEQAHSIK
PMKFSVSPVVRVAVEPANPKDLPKLLEGMKRLDKSDPCVMCICDKDENQN
IIAGAGELHLEICLKDLREDFCGGMDIRVSDPVVSYRETVTEKSTKVVMA
KSANKHNRLYFEAEPISEEVIEAIKDGEITSEQDSKVRARILTDKYGWDS
DEAKQIWSFGPVGASSGHMTNLILEATKGVQYVKESKEHIVSGFQIVCRN
GVLAGEELVGTCFKLRDATFHADAIHRGAGQLTPATRRGLYAYASPMLME
PFYLVDILAPEGCMGGIYSTMSKRRGVVISEEPREGQPLTEVKAHLPVAE
SFGFDADLRAATSGQAFPQCVFSHYALIPSSPLQTGSQAQGIMLSIRKRK
GMKEVVPDVSEYEDK
Ligand information
>8bsi Chain Lt (length=2593) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggcgggccugaggcggcgggggcgacgggcggaacuuaagcauaucagua
cgccccggaggagaaaccaaccgggauuccccgcuagcggcgagcgacgc
gggaggagcccgccccgaaggcgcgcuguggggccgcgagggggcccgag
ggccccgcccgagagggugcaagccccguacggcgaguagcgcugcuuga
gcgugcagcgcgaagggaggcgcggcccuuccaaggcuaaauacgccccg
ggaccgauagcggaccaaguagcgcgagcgaacggugaaaaggacgcccu
gcggccgcucaaaagaccugaacccggccggccgccggcccgccggcccc
gucucgaaacacggaccgaggagccacgcgccgcggcgagcccgagggag
cccccgcggcggagcgagcgcgagacgccccgggcccgccaugccccugc
gggcgugcgcgggccgagccgcggcgcgugggcccgaaaggcggugaucu
augcccggcgagggcgaggccgggcgaaagccugguggaggcccgccgcg
gugcugacgcgcagaucgcucgucggagccgggcaugggggcgaaagacu
caucgaaccgccugguagcugguugccuccgaaaugucucccaggacagc
cgccgccccgcaguugcggcccguagagcgcuggccggcgggagcggggg
gccugccccucgcccgccccccaaacuccgaagggccgcgccgccccgcc
gcuggccugggcggggcgggcgaaugcgggcggcgcgugggccccuccug
guaagcaggacgggcgaggcgggacgauccggacgccgggccagggugcg
ccgccggggcccgcggaacggcgucggccggucccgacagccgaaucggc
cggcccggaaaauggagcgcgccggagccccggacccgcgcccggccgcc
gcgcgcggcggguaggaggccgcagaggccccgggggcgaaggcggcgcg
caggccccgccggaccggccucuggugcagaucucggcagcaguagccgc
uacuccgcgccccggaggacugagggggagacggguuccgcggcgccugc
aucuggccgcgggugacucgggccuaagcggcgggugaagaccgggaagg
ggcgcccgcccgucgaacggggagccggcggagacuccggcaggcgcggc
ccccgcggagacgcccgccccccggcgacgcgcacggggaccgcggcggg
cggcgccccggcccgcgaacgccccgcagcccccggacgccuugcgcgga
gaggggggcccgggggcggaccgcgcguccccggccgccccugaaaagcc
ggggggcgccggccgcgcgccguaccgaccgcagcaggacuccgggguca
gcagccucuagcgcgggagcgaacgcggcucagggaagucggcaagccgg
cuccguaaccucgggaaaaggaguggcucugacggcgccgggucagaacu
ggaacggacgcggggaucccgacuguuuacuagaaacacagcgucgcgag
ggccgcacccggcgcuggcgcgacgugauuucugcccagugccacgaccg
ucaccgugaagcgauccgccgaagcccugguaaacggcgggaguaacuau
gacucucuuaagguagccaaaugccucgucgggcaauuuccgacgugcau
gaauggaccaacgaggaucccacugucccgagccgcgccuccgcgagccu
ccagccucgggaacgggcgagggccggccagcggggcaagaagacccuuu
ugagcuugacuccagcccgggccuguggggcggggcggccggcgcagcgc
acaggggaggccgcgccccugagacacccugacggccgccgccgccccgc
ucacccggucgcgcggggacccgcccgggcggggaguucggcuggggcgg
cgcgccugcuacaccggaccgcaggcgucccacggcgggcucagcgagga
cggagaccucccgcggagcagaagggcacaagcccgcccgacccgcgccc
cccgugccggcgcgggccgcgaaagcggggccuaccgauccuucgccgcc
ccggccgcgggcgcggagguggcagaaaaguuaccacagggauaacuggc
uuguggccgccgagcgcccgcagcgacgcggcuuuuugauccuucgaugu
cggcucuuccuaccguccgcgcgcaccggcgcggaagcgucggauuguuc
acccguucaagggaucgugagcuggguuuagaccgucgugagacagguua
guuuuacccuacuggccccggggccagagcacggcgggccaguacgagag
gaacgcccgccgcgggccgccagccccgcgguugcccggccgggcagcgc
cgcgccgccgcgcccgggggcccugcgcugaccgccucuaagcgcgcacc
ccgccucgcgccccgcccggccgcgcgccccagccccgugccccgucgcc
gagcggcccccgcccggggagaccacccggcgcggcgcuccuguacggcg
cagagcccugcgaucgccugagggacgcgccugcagagcgcgg
.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<.....<<.....>>.....
>>>>>..[[[...<<<.............>>><<<<....<<<<<.....
.>>>>>((......<<<.....))>>>...>>>>.....<<<<<<<....
....>>>>>>>...(((((..<<<<<<)))))..]]].............
.....................<<....>>................<<<..
..>>>............................<<<<<<..>>>>>>.<<
<<........>>>>.<<<<<....<<<<<<<<<<<<<..<<<...<<<..
..>>>..>>>...<<....>>............<<<<<<<<<<<<<....
>>>>>>>.>>>>>>...>>>>>>>>>>>>>..<<.<..<<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
<.<<.<<........>>>>>>>>....>>>>>>>>>...<<.......>>
......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><<
<<<<<<<<.<<<.<<<<<<<<<...<<<...<<...<<<<<<..<<<<<<
......>>>>>>>>>>>>...>>..>>.>...>>>>>>>.<<<<<<<<<<
.........>>>>>>>>>>...>>>>>>>>>>>>>.>>..<<<<.<<<<<
.....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((
...<<<<<<<........<<<.<<<<<<<<<..............>>>>>
>>>>>>>..........)))......>>>>>>>>>>>..>>>>>>><<<<
<....>>>>>....>>>>>><.<<<<<<<<...<<......<<<<<.<<.
...>>.>>>>>...>>>>>>>>>>..>.....>>>>>.<<.....<<...
..<<<<<.....>>>>>..<<.....<<<<..<<...<<<<<<...<...
....>.>>>>>>....((<<<.<<...<<<...<<<....>>>......<
<....>>...>>.>....>>...<<<<<........>>>>>))<<<<<<<
<...<<<....>>>..<<<<<<<<<<...<<<..<<<<<<<<<<<<<<<<
<...{{{...>>>>>....<<<<<<...<<<<<<....<<.....>>...
...>>>>>>...>>>>>>..>>>>>.>>>>>>>..>>>.........>>>
>>>>>>>...>>>>>>>>.>>>..>>..<<......>>.>>>>.<<<<..
...>>>>..>>....<<<<....<<<<<<<<.<<.........<.....<
<<<<....<<..>>.....>>>>><<.<<<<<<<<..>>>..>>>>>.>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
.<<<<....>>>>......>>>>>>>...<<..<<<..<<<<<<.<...<
<....<<<.<....>.>>>....>>.>>>>>..>>>>><<<<<<......
.>>>>>>........<...........<<<<<<.....>>>>>>.....>
.....>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>....>>
....<<<<<........>>>>>...((((((..{{{.........{{{..
{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<<<<<<<<.........
.<<<<<....................>>>>>.>>>>>>>>.>>>>>>>>>
...<<<<......>>>>.....>>>>>>>>..>>>>>...<<<.....>>
>..<<<<<<...........>>>>>>..<<...<<<<<<<...<.<<<<.
......>>>>.>.....<<...(((>>....>>>>>>>...<<<<<<<<.
........>>>>>>>>.<<<....>>>.>>)))......((((((.((((
(.>>>>>>))))))))))).........]]]]..}}.}}}.......<<.
<...<<<<<<...<<.......>>...>>>>>>....>.>.>.......<
<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<.......
..>>>....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.
.......}}}.))))))..<<<<<..<<<..<<<<<<<<...........
....>>>>>>>><<<<<...<.<<...<<<<<<<<<<}}}.>>>>>..>>
>>>...>>.>.>>>>>.<<<...<<<<<<...........>>>>>>...>
>>..>>>..>>>>>....<<<<<<..........................
..>>>>>>...<<.<<<<.......>>>>..<<<<<<<<<<<....<<<<
....<<...>>...>>>>...>>>>.>>>>>.>>.....>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8bsi Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.
Resolution3.4 Å
Binding residue
(original residue number in PDB)		H27 H143 C171 V172 R841 S845 Q847 Y894
Binding residue
(residue number reindexed from 1)		H25 H66 C94 V95 R759 S763 Q765 Y812
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003746 translation elongation factor activity
GO:0003924 GTPase activity
GO:0005525 GTP binding
GO:0043022 ribosome binding
Biological Process
GO:0006412 translation
GO:0006414 translational elongation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8bsi, PDBe:8bsi, PDBj:8bsi
PDBsum8bsi
PubMed36912103
UniProtA8BU59

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