Structure of PDB 8c95 Chain Z Binding Site BS01

Receptor Information

>8c95 Chain Z (length=58) Species: 562 (Escherichia coli)

AKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAV
SFMVKVEE

Ligand information

>8c95 Chain A (length=1220)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaccgggagauagcugg
uucuccccgaaagcuauuuagguagcgccucgugaauucaucuccggggg
uagagcagcaaacugcgaauaccggagaauguuaucacgggagacacacg
gcgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcu
aaggucccaaagucaugguuaagucggggcuaaaccaugcaccgaagcug
cggcagcgacgcuuaugcguuguuggguaggggagcguucuguaagccug
cgaaggugugcugugaggcaugcuggagguaucagaagugcgaaugcuga
cauaaguaacagacucagugaaauugaacucgcugugugcaguguccucc
uaaagaguaacggaggagcacgaagguuggcuaauccuggucggacauca
ggagguuagugcaauggcauaagccagcuugacugcgagcgugacggcgc
gagcaggugcgaaagcaggucauagugauccggugguucugaauggaagg
gccaucgcuaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<<<<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>....<<<<<.....<<....>
>......>>>>>...........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..<<<..<<<<......<<<<<<<<<<<<..<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>....>>>
>>>>>>>>>...>>>>........<<<.<<<...........>>>.>>>.
.....<<<<...((((.>>>>..>>>......>>.>>>>>>>.>>.....
........<.<<....>>...>>>.........<<<<.....>>>>....
.........>....>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>...<............>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<...<<<<<<.>>>>>>......>.>>
..<<<<<<....<<<<<....>>>>>..<<<<<<<...<<<<<<<<<...
..<<<.......>>.>.....>>>>>>>.>>...>>>>>>>.....<<<<
<<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<.
..<<<(((.....<<<<<<......))).....>>>>>>.>>>...>>>>
>>><<<<<<<<<....>>>>>>>>>....>>>>>><<<<<<<....<<..
....<<<<<<<<....>>>>>>>>...>>...>>>>>.>>.....>>>>>
>.......<<.......>>............<<<<....>>>>.<<<<<<
...........>>>>>>..<<...<<<<<<<...<<<<<<.......>>>
>>>.....<<...(((>>....>>>>>>>...<<<<..<<<<....>>>>
..>>>>.<<<....>>>.>>))).......<<<<<<<.<<..))))..>>
>>>>>>>....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c95 Cryo-EM captures early ribosome assembly in action.
• Resolution: 4.92 Å
• Binding residue (original residue number in PDB): R10 S11 I13 L16 K18 L24 G25 R29 R30 I31 R37 A42 M46
• Binding residue (residue number reindexed from 1): R10 S11 I13 L16 K18 L24 G25 R29 R30 I31 R37 A42 M46

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8c95, PDBe:8c95, PDBj:8c95
• PDBsum: 8c95
• PubMed: 36797249
• UniProt: P0AG51|RL30_ECOLI Large ribosomal subunit protein uL30 (Gene Name=rpmD)