Structure of PDB 7nhn Chain Z Binding Site BS01

Receptor Information

>7nhn Chain Z (length=73) Species: 169963 (Listeria monocytogenes EGD-e)

SNGRDSESKRLGAKRADGQFVTGGSILYRQRGTKIYPGTNVGRGGDDTLF
AKTDGVVRFERMGRDKKKVSVYP

Ligand information

>7nhn Chain A (length=2908)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaaga
aggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguuga
uccagagauuuccgaaugggggaacccacuaucuuuagucggauaguauc
cuuacgugaauacauagcgugaggaaggcagacccagggaacugaaacau
cuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggcg
agcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguuguag
gacacucuauacggaguuacaaaagaaaguuauaaaugaagcggucugga
aaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccuc
cagaguggauccugaguacggcggaacacgugaaauuccgucggaauccg
ggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacagu
uccugaaaccgugugccuacaaguaguuagagcccguuaaugggugauag
cgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguuaa
gcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaag
uaacaggucguagacccgaaaccaggugaucuacccauguccaggaugaa
gguaagguaauacuuacuggagguccgaacccacgcacguugaaaagugc
ggggaugagguguggguagcggagaaauuccaaucgaacuuggagauagc
ugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucaug
gagguagagcacuguuuggacuaggggcccuucucggguuaccgaauuca
gauaaacuccgaaugccauguacuuauacucgggagucagacugcgagug
auaagauccguagucgaaagggaaacagcccagaccaccaguuaaggucc
ccaaauauauguuaaguggaaaaggaugugggguugcuuagacaaccagg
auguuggcuuagaagcagccaccauugaaagagugcguaauagcucacug
gucgagugaccccgcgccgaaaauguaccggggcuaaacauauuaccgaa
acuguggaugaaccgguucgugguaggagagcguucuaagggcggugaag
ucagaccggaaggacugguggagcgcuuagaagugagaaugccgguauga
guagcgaaagaagggugagaaucccuuccaccgaauaucuaagguuuccu
gaggaaggcucguccgcucaggguuagucgggaccuaagccgaggccgau
aggcguaggcgauggacaacagguagagauuccuguaccagugcuaauug
uuuaaccgauggggugacacagaaggauagggaaucgcacgaauggaaau
gugcguccaagcagugagugugagaaguaggcaaauccgcuucucgcgaa
gcaugagcugugauggggaacggaaguuccugauuucacgcugucaagaa
aagccucuaggaagaguaguacugcccguaccgcaaaccgacacagguag
augaggagagaauccuaaggugagcgagagaacucucguuaaggaacucg
gcaaaaugaccccguaacuucgggagaaggggugcucuauuagggugcaa
gcccgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaaca
caggucucugcaaaaccguaaggugacguauaggggcugacgccugcccg
gugcuggaagguuaagaggagugcuuagcuucggcgaagguacgaauuga
agccccaguaaacggcggccguaacuauaacgguccuaagguagcgaaau
uccuugucggguaaguuccgacccgcacgaaaggcgcaacgaucugggca
cugucucaacgagagacucggugaaauuauaguaccugugaagaugcagg
uuacccgcgacaggacggaaagaccccguggagcuuuacugcaaccugau
auggaauguuuguaccgcuuguacaggauagguaggagccgaagagacgu
gugcgcuagcauacgaggaggcaauggugggauacuacccuggcuguaug
accauucuaacccgccacgcuuagcgcguggggagacagugucagguggg
caguuugacuggggcggucgccuccuaaagaguaacggaggcgcccaaag
guucccucagaauggauggaaaucauucgcagaguguaaaggcacaaggg
agcuugacugcgagacugacaagucgagcagggacgaaagucgggcuuag
ugauccggugguuccgcauggaagggccaucgcucaacggauaaaagcua
ccccggggauaacaggcuuaucucccccaagaguccacaucgacggggag
guuuggcaccucgaugucggcucgucgcauccuggggcuguagucggucc
caaggguugggcuguucgcccauuaaagcggcacgcgagcuggguucaga
acgucgugagacaguucggucccuauccgucgcgggcgcaggaaauuuga
gaggagcuguccuuaguacgagaggaccgggauggacacaccgcuggugu
accaguuguuccgccaggagcaucgcuggguagcuauguguggcagggau
aaacgcugaaagcaucuaagcgugaagccccccucaagaugagauuuccc
auuucuucggaaaguaagaucccugaaagaugaucagguagauagguuug
gaguggaaguguagcgauacauggagcggacaaauacuaaucgaucgagg
acuuaacc

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<<.....<<<<........<<<<<<<<<.<<<.....<<<<....
..>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]...........
....<<....>>....]].........<<<<....>>>>...........
..}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.......>>...>>>...<<....>>..<<<<.....>>>>......>
>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....
<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>
>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>
.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.....<<<<<<<<
<.....<<<...<<<<<<<<...<<<<<<<.....>>>>>.>>...>>>>
>>>>..>>.>.....>>>>>.>>>>...>>>>>>.>>..<<<<<<<.<<.
.....>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<((
(.....<<<<<<...........<<..<<<<<<<<<<.......<<<<<.
<<...<<<<.......>>>>...>>.........<<......>>...>>>
>>...>>>>>>>>>>.>>...........))).....>>>>>>.>>>...
>>>>>>><<<<<<..>>>>>>....>>>>>>..<<<<<<<.<<......<
<<<<.<<....>>.>>>>>...>>.>>>>>>>........>>>>>><...
...<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<
<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<...
.>>>...>>>...>>...<<<<<........>>>>>))<<<<<<<<.<<.
.....<<...<<<<<...<<<<..<<<..<<<<<.<<<<<...(((....
>>>>>.<<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....
>>>>..>>>>>...>>..........>>>>>...>>>...>>>>......
..>>>>>..>>..>>.>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.......
..<.....<<<<<.....<..>......>>>>><<<<<....<<<<....
>>>>...>>>>>............<<<<<<<<<..<<<.........>>>
..<<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..
<<<<<<<..<<<...<<...<<<<<..<<<..>>>...>>>>>...>>..
.>>>>>>>>..>>>>><<<<<<.......>>>>>>........<......
.....<<<<<<.....>>>>>>.....>....>>>>...>>.>>>>>>>.
.>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>
>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<
<<.<<<<<.<<<<<<.<<<...........<<<<<..<<<.......<<<
<<<<....>>>>>>>....>>>............>>>>>..>>>.>>>>>
>.>>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>
>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<...<
<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>>
>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))].
.....<<<<<<<.((..>>>>..))>>>>>>>.............>>>>.
.]]>>>>.......<<<<...<<<<<<...<<.......>>...>>>>>>
....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>
>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<
<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...
<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<<<..
.<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<..
..<<<<<...........>>>>>....>>>.>>>>.......>>>>>>>.
.<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<<<<<
..<<....<<<<<....>>>>>...>>...>>>>>.>>>...>>>>....
>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nhn Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): N20 R22 D23 S24 E25 S26 K27 R28 L29 K32 R33 A34 D35 G36 Q37 G41 G42 S43 I44 Y46 R47 R49 G50 T51 K52 Y54 G62 G63 D64 D65 F68 F77 K85
• Binding residue (residue number reindexed from 1): N2 R4 D5 S6 E7 S8 K9 R10 L11 K14 R15 A16 D17 G18 Q19 G23 G24 S25 I26 Y28 R29 R31 G32 T33 K34 Y36 G44 G45 D46 D47 F50 F59 K67

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nhn, PDBe:7nhn, PDBj:7nhn
• PDBsum: 7nhn
• PubMed: 34117249
• UniProt: P66125|RL27_LISMO Large ribosomal subunit protein bL27 (Gene Name=rpmA)