Structure of PDB 5uz4 Chain Z Binding Site BS01

Receptor Information

>5uz4 Chain Z (length=323) Species: 562 (Escherichia coli)

LSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIVISRFGMHADVE
SADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHERTS
VLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEP
IIVLNKIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEAL
TGRISIFAGQSGVGKSSLLNALLGLQKEILTNDISDARLYHFPHGGDVID
SPGVREFGLWHLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAV
EEGKIAETRFENYHRILESMAQV

Ligand information

>5uz4 Chain A (length=1527)

gaagaguuugaucauggcucagauugaacgcuggcggcaggccuaacaca
ugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacgagugg
cggacgggugaguaaugucugggaaacugccugauggagggggauaacua
cuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagaggggga
ccuucgggccucuugccaucggaugugcccagaugggauuagcuaguagg
ugggguaacggcucaccuaggcgacgaucccuagcuggucugagaggaug
accagccacacuggaacugagacacgguccagacuccuacgggaggcagc
aguggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgu
guaugaagaaggccuucggguuguaaaguacuuucagcggggaggaaggg
aguaaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccg
gcuaacuccgugccagcagccgcgguaauacggagggugcaagcguuaau
cggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugu
gaaauccccgggcucaaccugggaacugcaucugauacuagcaagcuuga
gucucguagagggggguagaauuccagguguagcggugaaaugcguagag
aucuggaggaauaccgguggcgaaggcggcccccuggacgaagacugacg
cucaggugcgaaagcguggggagcaaacaggauuagauacccugguaguc
cacgccguaaacgaugucgacuuggagguugugcccuugaggcguggcuu
ccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguu
aaaacucaaaugaauugacgggggcccgcacaagcgguggagcauguggu
uuaauucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaa
guuuucagagaugagaaugugccuucgggaaccgugagacaggugcugca
uggcugucgucagcucguguugugaaauguuggguuaagucccgcaacga
gcgcaacccuuauccuuuguugccagcgguccggccgggaacucaaagga
gacugccagugauaaacuggaggaagguggggaugacgucaagucaucau
ggcccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagag
aagcgaccucgcgagagcaagcggaccucauaaagugcgucguaguccgg
auuggagucugcaacucgacuccaugaagucggaaucgcuaguaaucgug
gaucagaaugccacggugaauacguucccgggccuuguacacaccgcccg
ucacaccaugggaguggguugcaaaagaaguagguagcuuaaccuucggg
agggcgcuuaccacuuugugauucaugacuggggugaagucguaacaagg
uaaccguaggggaaccugcgguuggau

...<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<.
<<<..<<<...<.<...<<<<<<<<<<....>>>>>>>.>>>.>..>.>>
>......<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<
<<......>>>>>......>>.>.....<<<....>>>....<<<<<<..
<<....>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<...<<<
<<<.........>>>>>>.>>>......>>>..<<<<<<<<....>>>>.
..>>>>.>>.<<<<.<<.........>>>>>>.<<<<....>>>>...>>
>>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..
<<<<......<<<<....>>>>.....>>>>...<.<<<<<......<.<
<<<<<.<.<...>.>.>>>>>>.>........>>>>>....>..<<<<<(
((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>
>..........<<<((.....<<<<...<<<..<<<<<<.<<<<<<<<<<
......<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>....<
<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......
>>>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>>...>
>>...>>>>....<..<<<...<<...<<<<.........>>>>...>>>
>>..>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>
>>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>
....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<...<<<<<<<<..
......<<........>>..........<<<<<<<......<<<<<<<..
<<<<<<<....>>>>>>>...<<....>>..>>>>>.>>.<<<.<<<..<
<<<<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>
>>.....<<<..<<<<<<....<...<<<.....>>>.>.....>>>>>>
.....<<<<<.....>>>>>.........>>>.........>>>...>>>
>>>>>>...>>>>>>>......>>>>>>>>.....<<<<<<<.....<<<
..<<...<<<....>>>...>>....>>>.....>>>>>>>......<..
..<<<<<<<........>>>>>>>....>......>>>>>.....<<<<<
<.........>>>>>>.......>>...>>>>>>>>>>.>>........<
<..<<<<.<....<<<<<<<..<<<....<<<<<..<<<...<<....>>
..>>>.>>>>>...>>>..>>>>>>>....>.>>>>..>.>.........
.<<<<<<<<<....>>>>>>>>>....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5uz4 The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly.
• Resolution: 5.8 Å
• Binding residue (original residue number in PDB): K8 Q10 N17 R47 F48 G49 D53 I66 R67 R68 T69 I70 D111 K117 D241 P268 G269 E272 Y299 R300 D307
• Binding residue (residue number reindexed from 1): K3 Q5 N12 R42 F43 G44 D48 I61 R62 R63 T64 I65 D106 K112 D236 P252 G253 E256 Y283 R284 D291

Enzymatic activity

• Enzyme Commision number: 3.6.1.-

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0019003 GDP binding
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0042254 ribosome biogenesis
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5uz4, PDBe:5uz4, PDBj:5uz4
• PDBsum: 5uz4
• PubMed: 28396444
• UniProt: P39286|RSGA_ECOLI Small ribosomal subunit biogenesis GTPase RsgA (Gene Name=rsgA)