Structure of PDB 7as8 Chain Y Binding Site BS01

Receptor Information

>7as8 Chain Y (length=101) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

MHVKKGDKVMVISGKDKGKQGTILAAFPKKDRVLVEGVNMVKKHSKPTQA
NPQGGISNQEAPIHVSNVMPLDPKTGEVTRVGYKVEDGKKVRVAKKSGQV
L

Ligand information

>7as8 Chain A (length=2812)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaug
aaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuug
auccggagauuuccgaauggggaaacccaccacucguaauggagugguau
ccauaucugaauucauaggauaugagaaggcagacccggggaacugaaac
aucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcgg
cgagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugu
aggacacucuguacggaguuacaaaggaacgagguagaugaagaggucug
gaaaggcccgccauaggagguaacagcccuguagucaaaacuucguucuc
uccugaguggauccugaguacggcggaacacgugaaauuccgucggaauc
cgggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaa
ccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaaaga
gauccugaaaccgugugccuacaaguagucagagcccguuaacgggugau
ggcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguu
aagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcaug
aguacguggucguagacccgaaaccaggugaucuacccauguccagggug
aaguucagguaacacugaauggaggcccgaacccacgcacguugaaaagu
gcggggaugagguguggguaggggugaaaugccaaucgaaccuggagaua
gcugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuu
ggagguagagcacugauuggacuaggggcccggguuaccgaauucaguca
aacuccgaaugccaaugacuuauccuugggagucagacugcgagugauaa
gauccguagucgaaagggaaacagcccagaccgccagcuaaggucccaaa
guauacguuaaguggaaaaggauguggaguugcuuagacaaccaggaugu
uggcuuagaagcagccaccauuuaaagagugcguaauagcucacuggucg
agugacucugcgccgaaaauguaccggggcuaaacguaucaccgaagcug
cggacuguucgaacagugguaggagagcguucuaagggcugugaagccag
accggaaggacugguggagcgcuuagaagugagaaugccgguaugaguag
cgaaagaggggugagaauccccuccaccgaaugccuaagguuuccugagg
aaggcucguccgcucaggguuagucgggaccuaagccgaggccgaaaggc
guaggcgauggacaacagguugauauuccuguaccaccuccucaccauuu
gagcaauggggggacgcaggaggauaggguaagcgcgguauuggauaucc
gcguccaagcaguuaggcugggaaauaggcaaauccguuucccaaaggcu
gagcugugauggcgagcgcgaaguuccugauuccacacugccaagaaaag
ccucuagcgaggugagaggugcccguaccgcaaaccgacacagguaggcg
aggagagaauccuaaggugaucgagagaacucucguuaaggaacucggca
aaaugaccccguaacuucgggagaaggggugcucuguuagggugcaagcc
cgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaacacag
gucucugcgaagccguaaggcgaaguauaggggcugacgccugcccggug
cuggaagguuaagaggagcgcuuagcguaagcgaaggugcgaauugaagc
cccaguaaacggcggccgucgguccuaagguagcgaaauuccuugucggg
uaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucucaacg
agagacucggugaaauuauaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccguggagcuuuacugcagccugauauugaauguug
guuugaccuucuaacccgccgcccuuaucgggcggggagacagugucagg
ugggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgccc
aaagguucccucagaaugguuggaaaucauucgcagaguguaaaggcaca
agggagcuugacugcgagaccuacaagucgagcagggacgaaagucgggc
uuagugauccggugguuccgcauggaagggccaucgcucaacggauaaaa
gcuaccccggggauaacaggcuuaucucccccaagaguccacaucgacgg
ggagguuuggcaccucgaugucggcucaucgcauccuggggcuguagucg
gucccaaggguugggcuguucgcccauuaaagcgguacgcgagcuggguu
cagaacgucgugagacaguucggucccuauccgucgcgggcgcaggaaau
uugagaggagcuguccuuaguacgagaggaccgggauggacgcaccgcug
guguaccaguuguucugccaagggcaucgcuggguagcuaugugcggacg
ggauaagugcugaaagcaucuaagcaugaagccccccucaagaugagauu
ucccauuccgcaaggaaguaagaucccugaaagaugaucagguugauagg
ucugagguggaaguguggcgacacauggagcugacagauacuaaucgauc
gaggacuuaacc

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<..[..((.>>>.....<<<<<<<..<<<))..]..>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<...
.>>......>>>>>.>.........<<....>>...<<<<......<<..
...>>.....>>>>...(((....<<<<<<<<....>>>>>>>>......
<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<..
....>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>
>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>.....
.<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].........
......<<....>>....]].........<<<<....>>>>.........
....}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>
>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<.
..<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....
.>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>
>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>.
.>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<
<<.....<<<...<<<<<<<<...<<<<<<..>>>>.>>...>>>>>>>>
..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<.....
.>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((...
..<<<<<<...........<<..<<<<<<<<<........<<<<<.<<..
.<<<<.......>>>>...>>.........<<......>>...>>>>>..
..>>>>>>>>>.>>...........))).....>>>>>>.>>>...>>>>
>>><<<<<<..>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<
.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<
<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<.
..<<.....>>.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>
...>>>..>>>...<<<<<........>>>>>))<<<<..<<<<<<....
..<<...<<<<....<<<<..<<<..<<<.<<<<<<<<...(((....>>
>>>><<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>...>>>>
..>>>>>...>><..<..>...>>>>>>...>>>...>>>>.........
>>>>..>>..>>>>>>.>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.........<
.....<<<<<....<....>.....>>>>><<<<<....<<<<....>>>
>...>>>>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<
<<<<..<<<<..<<...<<<<<<.<<....>>..>>>>>>...>>..>>>
>>>>>>..>>>>><<<<<.>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>...>>.>>>>>>>..>..>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<.<<<<
...>>>>.>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>
>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<
...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>...
.>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>)
))]......<<<<<<<.((..>>>>..))>>>>>>>.............>
>>>..]]>>>>.......<<<<...<<<<<<...<<.......>>...>>
>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>
>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>..
..<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<
<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<
<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<
<<....<<<<<...........>>>>>....>>>.>>>>.......>>>>
>>>..<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<
<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>
....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7as8 Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): H2 K4 I12 S13 G14 K15 K17 A26 K29 V41 K42 K43 H44 S45 K46 P47 G55 I56 S66 N67 E77 V78 R80 G82 Y83 K90 K95
• Binding residue (residue number reindexed from 1): H2 K4 I12 S13 G14 K15 K17 A26 K29 V41 K42 K43 H44 S45 K46 P47 G55 I56 S66 N67 E77 V78 R80 G82 Y83 K90 K95

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0009295 nucleoid
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7as8, PDBe:7as8, PDBj:7as8
• PDBsum: 7as8
• PubMed: 33259810
• UniProt: P0CI78|RL24_BACSU Large ribosomal subunit protein uL24 (Gene Name=rplX)