Structure of PDB 3bbx Chain Y Binding Site BS01

Receptor Information

>3bbx Chain Y (length=58) Species: 562 (Escherichia coli)

AKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAV
SFMVKVEE

Ligand information

>3bbx Chain B (length=2902)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaagggggucaucccgacuaccaac
ccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacggc
gggugcuaacguccgucgugaagagggaaacaacccagaccgccagcuaa
ggucccaaagucaugguuaagugggaaacgaugugggaaggcccagacag
ccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagcu
cacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugca
ccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguucu
guaagccugcgaaggugugcugugaggcaugcuggagguaucagaagugc
gaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgga
agaccaaggguuccuguccaacguuaaucggggcagggugagucgacccc
uaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccug
uacuugguguuacugcgaaggggggacggagaaggcuauguuggccgggc
gacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccgg
aaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaaca
aaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaaucg
uaccccaaaccgacacagguggucagguagagaauaccaaggcgcuugag
agaacucgggugaaggaacuaggcaaaauggugccguaacuucgggagaa
ggcacgcugauauguaggugaggucccucgcggauggagcugaaaucagu
cgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugca
aacacgaaaguggacguauacggugugacgccugcccggugccggaaggu
uaauugaugggguuagcgcaagcgaagcucuugaucgaagccccgguaaa
cggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgggu
aaguuccgaccugcacgaauggcguaaugauggccaggcugucuccaccc
gagacucagugaaauugaacucgcugugaagaugcaguguacccgcggca
agacggaaagaccccgugaaccuuuacuauagcuugacacugaacauuga
gccuugauguguaggauaggugggaggcuuugaaguguggacgccagucu
gcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaacg
uugacccguaauccggguugcggacagugucugguggguaguuugacugg
ggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaaucc
uggucggacaucaggagguuagugcaauggcauaagccagcuugacugcg
agcgugacggcgcgagcaggugcgaaagcaggucauagugauccgguggu
ucugaauggaagggccaucgcucaacggauaaaagguacuccggggauaa
caggcugauaccgcccaagaguucauaucgacggcgguguuuggcaccuc
gaugucggcucaucacauccuggggcugaaguaggucccaaggguauggc
uguucgccauuuaaagugguacgcgagcuggguuuagaacgucgugagac
aguucggucccuaucugccgugggcgcuggagaacugaggggggcugcuc
cuaguacgagaggaccggaguggacgcaucacugguguucggguugucau
gccaauggcacugcccgguagcuaaaugcggaagagauaagugcugaaag
caucuaagcacgaaacuugccccgagaugaguucucccugacccuuuaag
gguccugaaggaacguugaagacgacgacguugauaggccggguguguaa
gcgcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaac
cu

.<<<.<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..<<<..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<......>>>>>..>>>......>>.>>>>>>>.>>.....
..........<<....>>....>>.........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>..................<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<......
..>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.
.....>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<
<<<<<<.<...<<<...<<<.<<<<...<...<<.......>>...>...
>>>>.>>>..>>.>...>.>>>>>>.>>>...>>>>>>>.>...<<<<<<
<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<...
<<<(((.....<<<<<<...........<<..<<<<<..<<........<
<<<<.<<...<<<<.......>>>>...>>........<<<......>>>
..>>>>>....>>..>>>>>.>>...........))).....>>>>>>.>
>>...>>>>>>><<<<<<<<<....>>>>>>>>>....>>>>>>..<<<<
<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>....
....>>>>>>..............<<<<<<.......>>>>>><<<<...
.<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<<
...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>>
>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<.
...[[....>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>
>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>>
...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<
....<<<<<<<<.<<.........<.....<<<<<...............
>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
..<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<
<.......<<<<<..<<....>>...>>>>>.......>>>>>>>>>..>
>>>><<<<<<.......>>>>>>........<...........<<<<<..
.....>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>
>>>.>>>>.............<<<<<........>>>>>..<<<<<<<<.
<<<.<<......<<<<.<<<<<<..<<<<<.<<<.<<<<..<<<<<<<<<
<<.<<<<.........>.<<(.[..<<.......<<<<<<<<....>>>>
>>>>....>>............>>).]...>>>.>>>>>>>>>>>...<<
<.<<<<<<.....>>>>>>>>>.....>>>>.>>>..>>>>>..<<<<..
...>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<<
<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<.
.<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<<<
.<<........>>>>>>>>>.............>>>>..>>>>>>.....
..<<<<...<<<<<<...<<.......>>...>>>>>>....>>>.>...
....<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<
.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>
.>>>>....>>..>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<
<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<
.]].>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.....
......>>>>>....>>>.>>>>.......>>>>>>>..<<<<<<...>>
>>>>.....<<..<<<<<.......>>>>>....<<<<<<<<..<<....
<<<<<....>>>>>...>>...>>>>>.>>>.....>>.....>>>.>>>
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3bbx Recycling of Aborted Ribosomal 50S Subunit-Nascent Chain-tRNA Complexes by the Heat Shock Protein Hsp15.
• Resolution: 10.0 Å
• Binding residue (original residue number in PDB): R10 S11 A12 I13 G14 L16 P17 K20 A21 L23 L24 G25 L26 G27 L28 R29 R30 I31 H33 V35 R37 E38 P41 A42 I43 R44 G45 M46
• Binding residue (residue number reindexed from 1): R10 S11 A12 I13 G14 L16 P17 K20 A21 L23 L24 G25 L26 G27 L28 R29 R30 I31 H33 V35 R37 E38 P41 A42 I43 R44 G45 M46

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3bbx, PDBe:3bbx, PDBj:3bbx
• PDBsum: 3bbx
• PubMed: 19013177
• UniProt: P0AG51|RL30_ECOLI Large ribosomal subunit protein uL30 (Gene Name=rpmD)