Structure of PDB 3b0u Chain Y Binding Site BS01

Receptor Information
>3b0u Chain Y (length=312) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
LDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERLL
AFRPEEHPIALQLAGSDPKSLAEAARIGEAFGYDEINLNLGCPSEKAQEG
GYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSVE
AMAEAGVKVFVVHARSALLALSTKANREIPPLRHDWVHRLKGDFPQLTFV
TNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADRRVFGLPRRPSR
LEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGRS
LQALDRALRLME
Ligand information
Receptor-Ligand Complex Structure
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PDB3b0u Molecular basis of dihydrouridine formation on tRNA
Resolution1.948 Å
Binding residue
(original residue number in PDB)		M36 V38 A41 G45 N46 R49 L50 C93 P94 S95 K97 Y103 T174 K175 R178
Binding residue
(residue number reindexed from 1)		M35 V37 A40 G44 N45 R48 L49 C92 P93 S94 K96 Y102 T173 K174 R177
Enzymatic activity
Enzyme Commision number 1.3.1.-
1.3.1.91: tRNA-dihydrouridine(20) synthase [NAD(P)(+)].
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0050660 flavin adenine dinucleotide binding
GO:0102264 tRNA-dihydrouridine20 synthase activity
GO:0102266 tRNA-dihydrouridine20a synthase activity
Biological Process
GO:0002943 tRNA dihydrouridine synthesis
GO:0008033 tRNA processing

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Molecular Function
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Biological Process
External links
PDB RCSB:3b0u, PDBe:3b0u, PDBj:3b0u
PDBsum3b0u
PubMed22123979
UniProtQ5SMC7|DUSAL_THET8 tRNA-dihydrouridine(20/20a) synthase (Gene Name=dus)

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