Structure of PDB 8c01 Chain X Binding Site BS01

Receptor Information

>8c01 Chain X (length=141) Species: 4932 (Saccharomyces cerevisiae)

LTVQSERAFQKQPHIFNNPKVKTSKRTKRWYKNAGLGFKTPKTAIEGSYI
DKKCPFTGLVSIRGKILTGTVVSTKMHRTIVIRRAYLHYIPKYNRYEKRH
KNVPVHVSPAFRVQVGDIVTVGQCRPISKTVRFNVVKVSAA

Ligand information

>8c01 Chain 2 (length=1545)

aucugguugauccugccaguagucauaugcuugucucaaagauuaagcca
ugcaugucuaaguauaagcaaauacagugaaacugcgaauggcucauuaa
aucaguuaucguuuauuugauaguuacaugguauaacugugguaauucua
gagcuaauacaugcuuaaaaucucgaagagauguauuuauuagauaaaaa
uugaugauucauaauaacuuuucgaaucgcauggccgcuggcgaugguuc
auucaaauuucugcccuaucaacuuucgaugguaggauaguggccuacca
ugguuucaacggguaacggggaauaaggguucgauuccggagagggagcc
ugagaaacggcuaccacauccaaggaaggcagcaggcgcgcaaauuaccc
aauccuaauucagggagguagugacaauaaauaacgauacagguuguauu
ggaaugaguacaauguaaauaccuuaacgaggaacaauuggagggcaagu
cuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaagu
uguugcaguuaaaaagcucguaguugaacuuugggcccgguuggccaacg
gggccuuuuuuacuuugaaaaaauuagaguguucaaagcaggcgugcucg
aauauauuagcauggaauaauagaauaggacguuugguucuauuuuuuuc
uaggaccaucguaaugauuaauagggacggucgggggcaucaguauucaa
uugucagaggugaaauucuuggauuuauugaagacuaacuacugcgaaag
cauuugccaaggacguuuucauuaaucaagaacgaaaguuaggggaucga
agaugaucagauaccgucguagucuuaaccauaaacuaugccgacuaggg
aucgggugguguuuuuaaugacccacucggcaccuuacgagaaaucaaag
ucuuuggguucuggggggaguauggucgcaaggcugaaacuuaaaggaau
ugacagggcaccaccaggaguggagccugcggcuuaauuuggaaacucac
cagguccacacaauaagauugacagauugagagcucuuucuugauuuugu
ggguggugggccgguugguggagugauuugucugcuuaauugcgauaacg
aacgaccuuaaccacuaauaguggugcuagcauuugcugguuccacuucu
uagagggacuaggaaggaggggucccuuagacguucugggccgcacgcgc
gcuacacugacggagccagcgagucuaaccuuggccgagaggucuuggua
aucuugugaaacuccgucgugcuggggauagagcauuguaauuauugcuc
uucaacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuac
gucccugcccuguaccuaguaccgauugaauggcuuagugaggccucagg
aucugcuuagagucagagcggagaauuuggacaaacuuggucauuuagag
gaacuaagguuuccguaggugaaccugcggaaggaucauuaaaga

..<<<<<.{.((((>>>>><<<<.<<<<<<...<.<...<......<<<.
<<<..<<....<<....<<.......>>...>>.>>......<<......
..<<<..<<..<<....<<<........<<......<<.<<.......>>
.>>......>>........<<<<<...>>>>><.<.<<<<<<......((
...........>>>>>>.>...>...<<<<..<<<.>>>.>>>>....>>
>...>>>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>
>>>>......>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.
.........>>>.>>.<.<<<..>>>.>...>>>>>>.........<<<.
...<<<.....>>>..>>>..>....>.>.....<<<<<<<<.>>>>>>>
>......<<..<...........>..>>.........<<<<<((....<.
<<<<.....<<..))>>.......>>>>>>>>>>..>>>>>>>>>>....
.....<<<[[.....<.<<...<<<.<<....<<<<<<.<<<<..>>>>.
>>>>>>...<<<<<.<<.......<<...<.......>..<<<..>>>..
..>>......>>.>>..>>>.........<<<..<<<<<<<<.....)).
>>>>>>>>.>>>.....>>....<<<<<<.<<...<<<<..<<..<<<<<
<.<...<<<..(...>>>......>.>>>>>>..>>.......<<....>
>...>>>>...>>>>>.>>>...>>>...>>.>....<<<<<<<...<..
.<<<<..)......>>>>...>>>>>>>>..........<<<.<<.<<<.
.<<<<<<<<.<<<......>>>>>>>>>>>..>>>...<<..]]>>...>
>.....>>>.>>>.<<<......<<<<....>>>>....>>>..)))).}
....<<<<<<..<<.<<<<<<..<<<.<<<<<<................<
<<<<.<..<<<<<.......<<.<<<........>>>.>>......>>>>
>......<<<<<.<<<.<<<<......>>...<<<......>>>...>>.
>>>..<<<<..<<.<<<..<<<<<.<<<<<<<..>>>>>>>.>>>>>...
>>>.>>..........>>>>>>>>>.......>.>>>>>...>>.>>>>.
>>>.....<<<<<<<......<<.....<<..<<<<....>>>>..>>..
..>>.......>>>>>>>......<....<<<<<<..........>>>>>
>....>.....>>>>>>....<<<<<<<<.......>>>>>>>>......
>>...>>>>>>.....<<<<.<<....<<<<<<<<.<<<..<.<<..<..
.<<<<<<<.......>>>>>>>...>..>>.>..>>>.>>>>>>>>...>
>.>>>>....<<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c01 Impact of the yeast S0/uS2-cluster ribosomal protein rpS21/eS21 on rRNA folding and the architecture of small ribosomal subunit precursors.
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): P17 H18 W34 G39 L40 K57 I66 R67 K69 S77 M80 Y90 K96 Y97 K102 R103 H104 K105 N106 R129 P130 S132 K133 T134
• Binding residue (residue number reindexed from 1): P13 H14 W30 G35 L36 K53 I62 R63 K65 S73 M76 Y86 K92 Y93 K98 R99 H100 K101 N102 R125 P126 S128 K129 T130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8c01, PDBe:8c01, PDBj:8c01
• PDBsum: 8c01
• PubMed: 36996028
• UniProt: P0CX47|RS11A_YEAST Small ribosomal subunit protein uS17A (Gene Name=RPS11A)