Structure of PDB 5jus Chain WA Binding Site BS01

Receptor Information

>5jus Chain WA (length=318) Species: 4932 (Saccharomyces cerevisiae)

ASNEVLVLRGTLEGHNGWVTSLATSAGQPNLLLSASRDKTLISWKLTGDD
QKFGVPVRSFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQ
RFVGHKSDVMSVDIDKKASMIISGSRDKTIKVWTIKGQCLATLLGHNDWV
SQVRVVPNEKADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINT
LTASPDGTLIASAGKDGEIMLWNLAAKKAMYTLSAQDEVFSLAFSPNRYW
LAAATATGIKVFSLDPQYLVDDLRPEFAGYSKAAEPHAVSLAWSADGQTL
FAGYTDNVIRVWQVMTAN

Ligand information

>5jus Chain A (length=1781)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccggucggauuuccaacggggccuuuccuucuggcuaaccuugagucc
uuguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguu
caaagcaggcguauugcucgaauauauuagcauggaauaauagaauagga
cguuugguucuauuuuguugguuucuaggaccaucguaaugauuaauagg
gacggucgggggcaucaguauucaauugucagaggugaaauucuuggauu
uauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaa
ucaagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucu
uaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugacc
cacucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguau
ggucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggag
uggagccugcggcuuaauuugacucaacacggggaaacucaccaggucca
gacacaauaaggauugacagauugagagcucuuucuugauuuugugggug
guggugcauggccguucuuaguugguggagugauuugucugcuuaauugc
gauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauuugcug
guuauccacuucuuagagggacuaucgguuucaagccgauggaaguuuga
ggcaauaacaggucugugaugcccuuagacguucugggccgcacgcgcgc
uacacugacggagccagcgagucuaaccuuggccgagaggucuugguaau
cuugugaaacuccgucgugcuggggauagagcauuguaauuauugcucuu
caacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacgu
cccugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuua
gugaggccucaggaucugcuuagagaagggggcaacuccaucucagagcg
gagaauuuggacaaacuuggucauuuagaggaacuaaaagucguaacaag
guuuccguaggugaaccugcggaaggaucau

...<<<<<.{.[[[[>>>>><<<<.<<<<<<.....<..........<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..................<<....<.
..<.......>...>.....>>.........<<<<<...<....>...>>
>>><...<<<<<.......((((((..<<......>>.............
..>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>
>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.......
...>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<
.....>>>..>>>.......>.......<<<<<<<<<<<<......>>>>
>>>>>.>>>......<<..<...........>..>>.........<<<<<
((....<.<<<<.....<<..))>>.......>>>>>>>>>>..>>>>>>
>>>>.........<<<[[.....<.<<...<<<.<<....<<<<<<.<<<
<.....<..>.....>>>>.>>>>>>.....<<<<<........<<....
.......>>........>>>>>.....<<<<.<<.......<<...<...
....>..<<<.....>>>....>>......>>.>>..>>..........<
<...<<<<<<<.......))))))..>>>>>>>..>>.....>>....<<
<<<<.<<...<<<<...<<.<.<<<<.<...<<........>>......>
.>>>>..>>>........<<....>>...>>>>..>.>>>>.>>>...>>
>...>>.>....<<<.<.....<...<.<...........>.>...>..>
.>>>..........<<<.<<..<<<.<<<<<<<<.<<<........>>>>
>>>>>>>..>>>...<<..]]>>...>>.....>>>.>>>.<<<......
<<<......>>>....>>>..]]]].}<<<<<.<<<<<<<..<<..<<<<
...<<<.<<<<<<......<..........>..........<<<<<....
..<<<<<........<<..<<........>>..>>......>>>>>...<
<.<<<..<<.<<<<<.....<<<.<<<<<....>>>...<<<......>>
>...>>.>>>....<<<<..<<..<<<<..<<<<<.<<<<<<....>>>>
>>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>........>>
>>.....>>.>>>.....>>>>>>>.......>>>>>...>>.>>>>.>>
>.....<<<<<<<......<<.....<<..<<<<....>>>>..>>....
>>.......>>>>>>>...........<<<<<<..........>>>>>>.
..........>>>>.....<<<<<<<<.......>>>>>>>>......>>
...>>>>>>>>>>.>>.......<<....<<<<.<<....<<<<<<<<.<
<<..<.<<..<...<<<<<<...<<<.<<.......>>..>>>...>>>>
>>...>..>>.>..>>>.>>>>>>>>...>>.>>>>.....>>.......
...<<<<<<<......>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5jus Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.
• Resolution: 4.2 Å
• Binding residue (original residue number in PDB): G63 S65 H66 R90 R102 S282 A285
• Binding residue (residue number reindexed from 1): G62 S64 H65 R89 R101 S281 A284

Gene Ontology

Molecular Function

• GO:0001965 G-protein alpha-subunit binding
• GO:0005080 protein kinase C binding
• GO:0005092 GDP-dissociation inhibitor activity
• GO:0043022 ribosome binding
• GO:0045182 translation regulator activity

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006521 regulation of cellular amino acid metabolic process
• GO:0007186 G protein-coupled receptor signaling pathway
• GO:0010629 negative regulation of gene expression
• GO:0017148 negative regulation of translation
• GO:0061157 mRNA destabilization
• GO:0070651 nonfunctional rRNA decay
• GO:0072344 rescue of stalled ribosome
• GO:1902660 negative regulation of glucose mediated signaling pathway
• GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process
• GO:1990145 maintenance of translational fidelity
• GO:2001125 negative regulation of translational frameshifting

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5jus, PDBe:5jus, PDBj:5jus
• PDBsum: 5jus
• PubMed: 27159452
• UniProt: P38011|GBLP_YEAST Small ribosomal subunit protein RACK1 (Gene Name=ASC1)