Structure of PDB 7of1 Chain W Binding Site BS01

Receptor Information

>7of1 Chain W (length=227) Species: 559292 (Saccharomyces cerevisiae S288C)

RSKRSKLVTLAQTDKKGRENKERIFDEVREALDTYRYVWVLHLDDVRTPV
LQEIRTSWAGSKLIMGKRKVLQKALGEKREEEYKENLYQLSKLCSGVTGL
LFTDEDVNTVKEYFKSYVRSDYSRPLTFTIPEGIVYSRGGQIPAEEDVPM
IHSLEPTMRNKFEIPTKIKAGKITIDSPYLVCTEGEKLDVRQALILKQFG
IAASEFKVKVSAYYDNDSSTVESTNIN

Ligand information

>7of1 Chain 1 (length=2787)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaggccagcaucag
uuuugguggcaggauaaauccauaggaauguagcuugccucgguaaguau
uauagccugugggaauacugccagcugggacugaggacugcgacguaagu
caaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacc
aaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcgua
augaaagugaacguagguuggggccucgcaagaggugcacaaucgaccga
uccugaugucuucggauggauuugaguaagagcauagcuguugggacccg
aaagauggugaacuaugccugaauagggugaagccagaggaaacucuggu
ggaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguau
aggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguu
ucccucggauagcagaagcucguaucaguuuuaugaguaaauauguaaga
aguccuuguuuaauugaacguggacauuugaaugaagagcuuuuaguggg
ccauuuuugguaagaacuggcgaugcgggaugaaccgaacguagaguuaa
ggugccggaauacacgcucaucagacaccacaaaagguguuaguucaucu
agacagccggacgguggccauggaagucggaauccgcuaaggagugugua
acaacucaccggccgaaugaacuagcccugaaaauggauggcgcucaagc
guguuaccuauacucuaccgucaggguuggaugcccugacgaguaggcag
gcguggaggucagugacgaagccuagaccguaaggucgggucgaacggcc
ucuagugcagaucuuggugguaguagcaaauauucaaaugagaacuuuga
agacugaaguggggaaagguuccacgucaacagcaguuggacguggguua
gucgauccuaagagauggggaagcuccguuucaaaggccugaugcaggcc
accaucgaaagggaauccgguuaagauuccggaaccuggauauggauucu
ucacgguaacguaacugaauguggagacgucggcgcgagcccugggagga
guuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccgga
gauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggugc
gcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugccag
gucguacugauaaccgcagcaggucuccaaggugaacagccucuaguuga
uagaauaauguagauaagggaagucggcaaaauagauccguaacuucggg
auaaggauuggcucuaagggucggacgaucaacuuagaacuggacggaca
aggggaaucugacugucuaauuaaaacauagcauugcgauggucagaaag
ugauguugacgcaaugugauuucugcccagugcugucaaagugaagaaau
ucaaccaagcgcggguaaacggauaacgagauucccacugucccuaucua
cuaucuagcgaaaccacagccaagggaacgggcuuggcagaaucagcggg
gaaagaccugagcuugacucuaguuugacauugugaagagacauguuucu
uuacuuauucaaugaagcggagcuggaaccacguucuagcauucaagguc
ccauucggggcugauccggguugaagacaugucagguggggaguuuuagu
cccucggaauuugaggcuagaggaaaaguuaccacaggaagcuuguggca
gucaagcgacauugcuaugucggcucuuccuaucauaccgaagcagaauu
cgguaagcguuggauuguucacccacuaauagggaacgugagcuggguuu
agaccgucgugagacagguuaguacccuacugaugaauguuaccgcaaua
guaauugaacuuaguacgagaggaacaguucauucggauaauugguuuuu
gcggcugucugaucaggcauugccgcgaagcuaccauccgcuggauuaug
gcugaacgccucuaagucagaauccaugcuagaacgcggugauuucuuug
cuccacacaauauagauggauacgaauaaggcguccuuguggcgucgcug
aaccauagcaggcuagcaacggugcacuuggcggaaaggccuugggugcu
ugcuggcgaauugcaaugucauuuugcguggggauaaaucauuuguauac
gacuuagauguacaacgggguauuguaagcaguagaguagccuguuguua
cgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.((......<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<<<<<.<<<
<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>..
.....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>
>.....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<
<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...
<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<.
<<<...<......>...>>>..>>.>>....>>>>>>.>>>>>>..<<.<
...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>
.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>>>
...<<.......>>......>>>>>>......>.>>..<<<<<<....<.
<<<...>>>.><<<<<<<<<<...<<<.<<<<<<<........>>>>>..
.<<<<.<<<........>>>.>>>>.....>>>>>.>>>>>>>>.>>..<
<<<.<<<<....>>>>.>>>>....<<<......>>>...<<<<<<<..<
<<<(((.....<<<<<<.....<<..........<<<.<<<<<<<<<<..
.....<<<<<.<<<...<<.........>>...>>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>
>>>>.>>>>..>>>>>>><<<<<<<<<.....>>>>>>>>>....>>>>>
><.<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>
>>>>..>.....>>>>>><<.....<<.....<<<<<<.......>>>>>
><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....
((<<<<<<...<<....<<<....>>>.........<<<<<...>>>>>.
....>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<...
..<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<.<<<<<<<<<
<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>..
..>>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>...
>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>>>>>
>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..
<<<<.....<<<<<<<.<<...............<<<<<.....<..>..
....>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>.........
..<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....
>>>>......>>>>>>>........<<..<<<<.<<.....<<<<....>
>>>.....>>....>>..>>>>....>>.>>>>>>>.....>>.>>>>>>
>..>>>>>>....>>....<<<<<........>>>>>....(((((..[[
[.........((((..<<<<<.<<<<<<<<..<<<<<<.<<<..>>>.>>
>>>>...<<<<<.....<<<<..<<<..>>>..........}}...<<<<
<<....>>>>>>..>>>>..>>>>>....>>>>>>>>..>>>>>..[[[.
.((((.>>>>>>))))]]].......))))................<<<<
<<........>>>>>>.....<<<.<<<<<<..<.<<<<<<<......>>
>>>>>...>.<<<.........>>>.....>>>>>..>...>>>......
.<<<<<<<....>>>.>>>>.....]]].)))))....<<<<<<<<..<<
<<.<.<<<<<<...............>>>>>>.><<<<<...<<<<....
<<<<<<<<<<]]].>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<
<<<...........>>>>>...>>>>.>>>>....>>>>>>>>.......
<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>>>....
..>>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>>>
.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<..<.....
..>..>.....<<<<<.<<<.<<<....<<<<....<<<<<.....>>>>
>...>>>>...>>>....>>>..>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7of1 Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): R3 S4 K5 R6 S7 K8 A13 Q14 T15 K17 K18 R20 N22 K23 I26 D47 R49 T50 P51 Q54 R57 M67 K69 R70 K71 K75 E79 V99 S160 K204
• Binding residue (residue number reindexed from 1): R1 S2 K3 R4 S5 K6 A11 Q12 T13 K15 K16 R18 N20 K21 I24 D45 R47 T48 P49 Q52 R55 M65 K67 R68 K69 K73 E77 V97 S153 K197

Gene Ontology

Molecular Function

• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0000055 ribosomal large subunit export from nucleus
• GO:0000956 nuclear-transcribed mRNA catabolic process
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0030687 preribosome, large subunit precursor
• GO:0032040 small-subunit processome

External links

• PDB: RCSB:7of1, PDBe:7of1, PDBj:7of1
• PDBsum: 7of1
• PubMed: 34813592
• UniProt: P33201|MRT4_YEAST Ribosome assembly factor MRT4 (Gene Name=MRT4)