Structure of PDB 7bt6 Chain W Binding Site BS01

Receptor Information

>7bt6 Chain W (length=234) Species: 559292 (Saccharomyces cerevisiae S288C)

MPRSKRSKLVTLAQTDKKGRENKERIFDEVREALDTYRYVWVLHLDDVRT
PVLQEIRTSWAGSKLIMGKRKVLQKALGEKREEEYKENLYQLSKLCSGVT
GLLFTDEDVNTVKEYFKSYVRSDYSRPNTKAPLTFTIPEGIVYSRGGQIP
AEEDVPMIHSLEPTMRNKFEIPTKIKAGKITIDSPYLVCTEGEKLDVRQA
LILKQFGIAASEFKVKVSAYYDNDSSTVESTNIN

Ligand information

>7bt6 Chain 1 (length=2962)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagacaggccagcauc
aguuuugguggcaggauaaauccauaggaauguagcuugccucgguaagu
auuauagccugugggaauacugccagcugggacugaggacugcgacguaa
gucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacacgga
ccaaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcg
uaaugaaagugaacguagguuggggccucgcaagaggugcacaaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
aacuuuaaauauguaagaaguccuuguuugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguagaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacucuaccgucaggguugauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuagacc
guaaggucgggucgaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uuccaucgaaagggaauccgguuaagauuccggaaccuggauauggauuc
uucacgguaacguaacugaauguggagacgucggcgcgagcccugggagg
aguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccgg
agauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggug
cgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcca
ggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguug
auagaauaauguagauaagggaagucggcaaaauagauccguaacuucgg
gauaaggauuggcucuaagggucggacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
agugauguugacgcaaugugauuucugcccagugcucugaaugucaaagu
gaagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacucuc
uuaagguagccaaauggauuaacgagauucccacugucccuaucuacuau
cuagcgaaaccacagccaagggggcuuggcagaaucagcggggaaagaag
acccuguugagcuugacucuaguuugacauugugaagagacauaguuucu
uuacuuauucaagacauugucagguggggaguuugggggcggcacaucug
uuaaacgauaacgcagauguccuaaggggggcucauggagaacagaaauc
uccaguagaacaaaaggguaaaagcccccuugauuuugauuuucagugug
aauacaaaccaugaaaguguggccuaucgauccuuuagucccucggaauu
ugaggcuagaggugccagaaaaguuaccacagggauaacuggcuuguggc
agucaagcguucauagcgacauugcuuuuugauucuucgaugucggcucu
uccuaucauaccgaagcagaauucgguaagcguuggauuguucacccacu
aauagggagugagcuggguuuagaccgucgugagacagguuaguuuuacc
cuacugaugaauguuaccgcaauaguaauugaacuuaguacgagaggaac
aguucauucggauaauugguuuuugcggcugucugaucaggcauugccgc
gaagcuaccauccgcuggauuauggcugaacgccucuaagucagaaucca
ugcuagaacgcggugauuucuuugcuccacacaauauagauggauacgaa
uaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggugca
cuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauuuug
cguggggauaaaucauuuguauacgacuuagauguacaacgggguauugu
aagcaguagaguagccuuguuguuacgaucugcugagauuaagccuuugu
ugucugauuugu

..........................<<<<<<.....<<....>>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>......
.>>....<<<<<..<<<<<..<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>....
..>>>>>.>>>>>........<<<<<<<.<....>.>>>>>>>.....((
(((..<<<<<<)))))..]]].............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<<.....<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>
.......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....
>>>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><
<<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.
..<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<
<<<<<....<....>....>>>>.>>.>>....>>>>>>.>>>>>>..<<
.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>
>>...<<.......>>......>>>>>>......>.>>..<<<<<<....
<.<<<....>>>.><<.<<.<......<<<.<<<<<<<............
...........>>>>>...<<<<..<<....>>..>>>>.....>>>>>.
....>.>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<<....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
.....>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<..
...<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<
<<..{{{..>>>>>><...<<<<<....<<<<<<....<<<.....>>>.
...>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>>>..
.>>>>>>>.>>>>.........>>>>>>..........>>>..>>>>>>>
>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>.
.<<<<.....<<<<<<<.<<.........<.....<<<<<....<<..>>
.....>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>...............<<.............<
<<<....>>>>..........>>...........<<.........>>...
..........<<..>>......>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<....>>>>>....[[[[[..{{{.......
.((((((..[[[[..<<<<<..<<.<.<<..<<<<<.............>
>>>>...<...>.....>>.>.>>...>>>>>......<.<..<<<<<<<
<...........>>>>>>>>.....<<<<<<<...<<<<<<.......>>
>>>>.....<<......>>....>>>>>>>...<<<.<<<<<.......>
>>>.>>>>.<<<....>>>...>.>...[...(((((...((((.>>>>>
>)))).)))))....].....]]]]...))))))..............<<
<<<...<<.......>>...>>>>>.................<<<<<...
<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>..
...>>>>.....>>>>>.....<<<<<<<....>>>.>>>>........}
}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<...............
>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>..>>>>>
...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>
.>>>>....>>>>>>>>.......<<<<<<.<<<.<.....<<.......
...<<<<<<.....>>>>>>......>>.......<<<<<<...<<<<<.
<..<<<......>>>..>.>>>>>.>>>>>>..............>.>>>
.>>>>>>..<<<<<<<.............>.....<<<<<<<<<.<<<..
..<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>>.>>
>>>.>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bt6 Structural insights into assembly of the ribosomal nascent polypeptide exit tunnel.
• Resolution: 3.12 Å
• Binding residue (original residue number in PDB): M1 P2 R3 S4 K5 R6 A13 T15 R20 K23 D47 R49 T50 Q54 R57 I66 M67 K69 R70 K71 K75 R126 I158 S160
• Binding residue (residue number reindexed from 1): M1 P2 R3 S4 K5 R6 A13 T15 R20 K23 D47 R49 T50 Q54 R57 I66 M67 K69 R70 K71 K75 R126 I158 S160

Gene Ontology

Molecular Function

• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0000055 ribosomal large subunit export from nucleus
• GO:0000956 nuclear-transcribed mRNA catabolic process
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0030687 preribosome, large subunit precursor
• GO:0032040 small-subunit processome

External links

• PDB: RCSB:7bt6, PDBe:7bt6, PDBj:7bt6
• PDBsum: 7bt6
• PubMed: 33037216
• UniProt: P33201|MRT4_YEAST Ribosome assembly factor MRT4 (Gene Name=MRT4)