Structure of PDB 7bl3 Chain W Binding Site BS01

Receptor Information

>7bl3 Chain W (length=76) Species: 83333 (Escherichia coli K-12)

TRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTL
FAKADGKVKFEVKGPKNRKFISIEAE

Ligand information

>7bl3 Chain A (length=2902)

guuguaagguuaagcgacuaagcguacacgguggaugcccuggcagucag
aggcgaugaaggacgugcuaaucugcgauaagcgucgguaaggugauaug
aaccguuauaaccggcgauuuccgaauggggaaacccaguguguuucgac
acacuaucauuaacugaauccauagguuaaugaggcgaaccgggggaacu
gaaacaucuaaguaccccgaggaaaagaaaucaaccgagauucccccagu
agcggcgagcgaacggggagcagcccagagccugaaucaguguguguguu
aguggaagcgucuggaaaggcgcgcgauacagggugacagccccguacac
aaaaaugcacaugcugugagcucgaugaguagggcgggacacgugguauc
cugucugaauauggggggaccauccuccaaggcuaaauacuccugacuga
ccgauagugaaccaguaccgugagggaaaggcgaaaagaaccccggcgag
gggagugaaaaagaaccugaaaccguguacguacaagcagugggagcacg
cuuaggcgugugacugcguaccuuuuguauaaugggucagcgacuuauau
ucuguagcaagguuaaccgaauaggggagccgaagggaaaccgagucuua
acugggcguuaaguugcaggguauagacccgaaacccggugaucuagcca
ugggcagguugaagguuggguaacacuaacuggaggaccgaaccgacuaa
uguugaaaaauuagcggaugacuuguggcugggggugaaaggccaaucaa
accgggagauagcugguucuccccgaaagcuauuuagguagcgccucgug
aauucaucuccggggguagagcacuguuucggcaagggggucacuuacca
acccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacg
gcgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcu
aaggucccaaagucaugguuaagugggaaacgaugugggaaggcccagac
agccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauag
cucacuggucgagucggccugcgcggaagauguaacggggcuaaaccaug
caccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguu
cuguaagccugcgaaggugugcugugaggcaugcuggagguaucagaagu
gcgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccg
gaagaccaaggguuccuguccaacguuaaucggggcagggugagucgacc
ccuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauucc
uguacuugguguuacugcgaaggggggacggagaaggcuauguuggccgg
gcgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaaucc
ggaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaa
caaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaau
cguaccccaaaccgacacagguggucagguagagaauaccaaggcgcuug
agagaacucgggugaaggaacuaggcaaaauggugccguaacuucgggag
aaggcacgcugauauguaggugaggucccucgcggauggagcugaaauca
gucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugug
caaacacgaaaguggacguauacggugugacgccugcccggugccggaag
guuaauugaugggguuagcgcaagcgaagcucuugaucgaagccccggua
aacggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgg
guaaguuccgaccugcacgaauggcguaaugauggccaggcugucuccac
ccgagacucagugaaauugaacucgcugugaagaugcaguguacccgcgg
caagacggaaagaccccgugaaccuuuacuauagcuugacacugaacauu
gagccuugauguguaggauaggugggaggcuuugaaguguggacgccagu
cugcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaa
cguugacccguaauccggguugcggacagugucugguggguaguuugacu
ggggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaau
ccuggucggacaucaggagguuagugcaauggcauaagccagcuugacug
cgagcgugacggcgcgagcaggugcgaaagcaggucauagugauccggug
guucugaauggaagggccaucgcucaacggauaaaagguacuccggggau
aacaggcugauaccgcccaagaguucauaucgacggcgguguuugucggc
ucaucacauccuggggcugaaguaggucccaaggguauggcuguucgcca
uuuaaagugguacgcgagcuggguuuagaacgucgugagacaguucgguc
ccuaucugccgugggcgcuggagaacugaggggggcugcuccuaguacga
gaggaccggaguggacgcaucacugguguucggguugucaugccaauggc
acugcccgguagcuaaaugcggaagagauaagugcugaaagcaucuaagc
acgaaacuugccccgagaugaguucucccugacccuuuaaggguccugaa
ggaacguugaagacgacgacguugauaggccggguguguaagcgcagcga
ugcguugagcuaaccgguacuaaugaaccgugaggcuuaaccuuacaacg
cc

<<<<<<<<<<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<
<<<<......<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..]
.>>>......>>>>>>>...>>>....<<<....>>><<<<<<<....>>
>>>>>.<<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....
<<....>>......>>>>>.>.........<<....>>..<<<<<<....
.<<.....>>....>>>>>>..<<<..<<<<......<<<<<<<<<<<<<
.<<<...<<<<<......>>>>>((......<<<.....))>>>...>>>
...>>>>>>>>>>>>>...>>>>........<<<<<<<<.........>>
>>>>>>......<<<<<..<<<<>>>>>..>>>......>>.>>>>>>>.
>>...............<<....>>....>>.........<<<<.....>
>>>.............>....>>>>>>>>>>>.......<<<<..<<<<<
......>>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<
<<<<<<<<..<<<...<<.....>>...>>>...<<....>>..<<<<..
...>>>>.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<
<<<<..<<<<...<<<<<<......>>>>>>...>.>>>...<<<.<<<<
<........>>>>>>>>....>>>>>>>>>...<<<.....>>>......
>>>>>>......>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<
<...<<<<<<<<<.....<<<...<<<.<<<<...<<<<<<.>>>>.>>.
..>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<
<<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<.
..<<<(((.....<<<<<<...........<<..<<<<<..<<.......
.<<<<<.<<...<<<.........>>>...>>........<<<......>
>>..>>>>>....>>..>>>>>.>>...........))).....>>>>>>
.>>>...>>>>>>><<<<<..<<....>>..>>>>>....>>>>>><.<<
<<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>..
>.....>>>>>><......<<.....<<<<<<.......>>>>>><<<<.
...<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<
<<...<<<...<<<....>>>...>>>...>>>..<<<<<........>>
>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<
<....(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>
>>>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>
>>...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>
>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<
<<....<<<<<<<<.<<...............<<<<<....<<..>>...
..>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>
>>............<<<<<<<<<..<<<.........>>>..<<<<<<<.
....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<
<<........<<<<<<.<<....>>..>>>>>>........>>>>>>>>.
.>>>>>.<<<<<.......>>>>>.........<...........<<<<<
<.....>>>>>>.....>....>>>>>..>>.>>>>>>>.....>>.>>>
>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<
<.<<<.<<.....<<<<<.<<<<<<..<<<<<.<<<.<<<<<.<<<<<<<
<<<<.<<<<...........<<<<<..<<<......<<<<<<<<....>>
>>>>>>...>>>............>>>>>..>>>>.>>>>>>>>>>>...
<<<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<.
......>>><<<<<<...........>>>>>>..<<...<<<<<<<...<
<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<
<..<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<
<<.<<..>>>>..>>>>>>>>>.............>>>>..>>>>>>>..
...........<<<<<<...<<.......>>...>>>>>>.....<<<<<
..<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>
>.....>>>>>......>>>.>>....<<<<<<<....>>>.>>>>....
>>..>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<........
.......>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))).>>>>>
>..>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>
>>....>>>.>>>>.......>>>>>>>..<<<<<.....>>>>><....
<<..<<<<<.......>>>>>....<<<<<<<<..<<....<<<<<....
>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>>>>>>>>>.
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bl3 Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): T6 R7 N8 R10 S12 E13 K15 R16 L17 K20 F22 G23 E25 A29 G30 S31 I32 R37 G38 T39 F41 G50 R51 D52 H53 F56 K58 F65 K71 R73
• Binding residue (residue number reindexed from 1): T1 R2 N3 R5 S7 E8 K10 R11 L12 K15 F17 G18 E20 A24 G25 S26 I27 R32 G33 T34 F36 G45 R46 D47 H48 F51 K53 F60 K66 R68

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0043022 ribosome binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0001558 regulation of cell growth
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042256 cytosolic ribosome assembly
• GO:0090070 positive regulation of ribosome biogenesis
• GO:1902626 assembly of large subunit precursor of preribosome

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7bl3, PDBe:7bl3, PDBj:7bl3
• PDBsum: 7bl3
• PubMed: 33639093
• UniProt: P0A7L8|RL27_ECOLI Large ribosomal subunit protein bL27 (Gene Name=rpmA)