Structure of PDB 3j81 Chain W Binding Site BS01

Receptor Information

>3j81 Chain W (length=129) Species: 28985 (Kluyveromyces lactis)

TRTSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLQVMQKHGYIGEFE
YIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIADVEKWTANLLPARQFGY
VILTTSAGIMDHEEAHRKHVSGKILGFVY

Ligand information

>3j81 Chain 2 (length=1780)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacauggauaucug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagauaaaaaaucaaugucuucggacuccuugaugauuca
uaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaa
uuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuc
aacggguaacggggaauaaggguucgauuccggagagggagccugagaaa
cggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccua
auucagggagguagugacaauaaauaacgauacagggcccauucgggucu
uguaauuggaaugaguacaauguaaauaccuuaacgaggaacaacuggag
ggcaagucuggugccagcagccgcgguaauuccagcuccaguagcguaua
uuaaaguuguugcaguuaaaaagcucguaguugaacuuugggucugguug
uccggucgguuuuucaaccggaucuuuccuucuggcuaaccuguacuccu
ugugggugcaggcgaaccaggacuuuuacuuugaaaaaauuagaguguuc
aaagcaggcgaaagcucgaauauauuagcauggaauaauggaauaggacg
uuugguucuauuuuguugguuucuaggaccaucguaaugauuaauaggga
cggucgggggcaucaguauucaauugucagaggugaaauucuuggauuua
uugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaauc
aagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucuua
accauaaacuaugccgacuagggaucgggugguguuuuucuuaugaccca
cucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguaugg
ucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggagug
gagccugcggcuuaauuugacucaacacggggaaacucaccagguccaga
cacaauaaggauugacagauugagagcucuuucuugauuuuguggguggu
ggugcauggccguucuuaguugguggagugauuugucugcuuaauugcga
uaacgaacgagaccuuaaccuacuaaauaggguugcuggcacuugccggu
ugacucuucuuagagggacuaucgguuucaagccgauggaaguuugaggc
aauaacaggucugugaugcccuuagacguucugggccgcacgcgcgcuac
acugacggagccagcgaguacaaccuuggccgagaggucuggguaaucuu
gugaaacuccgucgugcuggggauagagcauuguaauuauugcucuucaa
cgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacguccc
ugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuuagug
aggccucaggauuugcuuagagaagggggcaacuccaucucagagcgaag
aaucuggucaaacuuggucauuuagaggaacuaaaagucguaacaagguu
uccguaggugaaccugcggaaggaucauua

...<<<<<...[[[[>>>>>[[[..((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[..................<.<..<<.
<<.......>>.>>.....>>.........<<<<<..<<....>>..>>>
>>[...((((((......<<<<<<<<<<<....>>>..............
)))))).....]...<<<<..<<<.....>>>.>>>>....]]]...}}}
}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>...
...>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<........
..>>>.>>.<.<<....>>..>..]]]]]].........<<<....<<<.
....>>>..>>>...............<<.<<<<<<............>>
>>>>..>.>.....<<.................>>.........<<<<<(
(......<<<<.....<<..))>>.......>>>>.>>>>>..)))))).
]]].........[[[{{.......{{...[[[.[[.....<<<<<<<<<<
.....<<>>.....>>>>>>>>>.>....<<<<<<....<.<.<..<...
....>..>.>.>....>>>>>>...<<<<<.<<.......<<...<....
...>..<<.......>>...>>......>>.>>..>>>.........[[.
..((((((((....>>>>>>>>.))))))))..]].....]]....<<<<
<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>......>.>
>>>>>..>>.......<<....>>...>>>>...>>>>>.>>>...]]].
..}}......<<<<<<<...<...<<<<.<.....>.>>>>...>>>>>>
>>..........<<<.<<.<<<..<<<<<<<<.<<.<.......>>>>>>
>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<......<<
<<....>>>>....>>>..]]]]..<<<<<.<<<<<<<..<<.<<<<<<.
.<<<.<<<<<<......<<........>>..........<<<<<.<....
<<<<<.....<..<<..<<........>.>.>>.>....>>>>>...<<.
<<<..<<.<<<<<<....<<<.<<<<......>>...<<<......>>>.
..>>.>>>....<<<<<.<<..<<<<..<<<<<<<<<<<<....>>>>>>
.>>>>>>..>>>>.>>....<<<<<<.....>>>>>>.......>>>>>.
...>>>.>>>.....>>>>>>>.....>.>>>>>...>>.>>>>.>>>..
...<<<<<<<......<<.....<<<.<<<<....>>>.>>>>....>>.
......>>>>>>>......<....<<<<<<..........>>>>>>....
>.....>>>>>>....<<<<<<<<.......>>>>>>>>......>>...
>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<.<<<.<.<<<.
.<<<<..<...<<<<<....<..................>....>>>>>.
..>..>>>>..>>>.>.>>>.>>...>>.>>>>...>.>>...>.....<
<<<<<<<<<..>>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j81 Structural changes enable start codon recognition by the eukaryotic translation initiation complex.
• Resolution: 4.0 Å
• Binding residue (original residue number in PDB): T2 R3 T4 V6 D9 N16 T20 K22 R28 S31 K32 R57 K60 K71 I75 S76 P77 R78 N80 V81 K82 I83 K88 W89 N92 L93 T105 T106 S107 I110 E115 R118 K119 S122
• Binding residue (residue number reindexed from 1): T1 R2 T3 V5 D8 N15 T19 K21 R27 S30 K31 R56 K59 K70 I74 S75 P76 R77 N79 V80 K81 I82 K87 W88 N91 L92 T104 T105 S106 I109 E114 R117 K118 S121

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j81, PDBe:3j81, PDBj:3j81
• PDBsum: 3j81
• PubMed: 25417110
• UniProt: Q6CW21|RS22_KLULA Small ribosomal subunit protein uS8 (Gene Name=RPS22)