Structure of PDB 7a5f Chain V6 Binding Site BS01

Receptor Information

>7a5f Chain V6 (length=328) Species: 9606 (Homo sapiens)

DSHKWEAREKEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEI
DHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQY
GIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYV
LFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVE
LQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASEALDVLGAVLKALTKL
VEQLDIEETEQSKLPQYLERFKALHSKLQALGLTTQLVKEKLSTCEAEDI
ATYEQNLQQWHLDLVQLIQREQQQREQA

Ligand information

>7a5f Chain A6 (length=928)

auagguuugguccuagccuuucuauuagcucuuaguaagauuacacaugc
aagcauccccguuccagugaguucacccucuaaaucgaucaaaaggaaca
agcaucaagcacgcagcaaugcagcucaaaacgcuuagccuagccacacc
cccacgggaaacagcagugauuaaccuuuagcaauaaacgaaaguuuaac
uaagcuauacuaaccccaggguuggucaauuucgugccagccaccgcggu
cacacgauuaacccaagucaauagaaccggcguaaagaguguuuuagauc
acaauaaagcuaaaacucaccugaguuguaaaaaacuccaguugacacaa
aauagacuacgaaaguggcuuuaacauaucugaacacacaauagcuaaga
cccaaacugggauuagauaccccacuaugcuuagcccuaaaccucaacag
uucaacaaaacugcucgccagaacacuacgagccacagcuuaaaacucaa
aggaccuggcggugcuucauaucccucuagaggagccuguucuguaaucg
auaaaccccgaucaaccucaccaccucuugcucagccuauauaccgccau
cuucagcaaacccugaugaaggcuacaaaguaagcgcaaguacccacgua
aagacguuaggucaagguguagcccaugagguggcaagaaaugggcuaca
uuuucuaccccagaaacgauagcccuuaugaaauaagggucgaaggugga
uuuagcaguaaacuaagaguagagugcuuaguugaacagggcccugaagc
gcguacacaccgcccgucacccuccucaaguauacuucaaaggacaauua
acuaaaaccccuacgcauuuauauagaggagacaagucguaacaugguaa
guguacuggaaagugcacuuggacgaac

..((((..<<<<<<.)))).<<<<<<.<<..<<<<<<<<..<.<<<.<<<
..<......>......<<........<<<.<..<<..>>.>..>>>...<
<<<....<<...<<<....>>>..>>......>>>>.<<...>>.>>.<<
<....>>>.....>>>>>>>........<<<....<<<<....>>>>..>
>>..>>.>>>>>>.....<<<<<<<<.....(((.....((.>>>))...
....)))..>>>>>..>>.>>>>>>...<<<((.....<<<<..<<<<..
....<<<<<<......<<<.<<<.<<<......>>>..>>>.>>>.....
........<<....>>.>>>>>>.....>>>>...>>>>....<<<<<<.
..<<...<<<<.<.....>.>>>>...>>>>>>>>..........<.<<<
<<..))..>>>>>.>.>>>.<<.......<<<<....>>>>.....>>..
>>>>>><<<<<.<<<<<<<...<<<.<<<<<..<<<<<<<<<<.....<<
........>>..........<<<<<<<.......<<.<<<..<<<<<<..
.....<<..<<<<<.....>>>.......>>..>>.....<<<....>>>
.........>>>...>>>>>>>>....>>>>>>>..>>.>>>>>>>>...
..<<<<.....>>>>.<....<<<<<<<<...>>>>>>>>....>.....
>>>>>.....<<<<<<<.........>>>>>>>.....>>>...>>>>>>
>>>>.>>....<..<<.<...<<<<<<..<<<<<......<<<....<<.
....>>...>>>.......>>>>>.>>>>>>....>.>>...>.....<<
<<<<<<<....>>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7a5f Elongational stalling activates mitoribosome-associated quality control.
• Resolution: 4.4 Å
• Binding residue (original residue number in PDB): L65 P66 S68 W102 Y103 R105 R392 Q396
• Binding residue (residue number reindexed from 1): L30 P31 S33 W67 Y68 R70 R320 Q324

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0097177 mitochondrial ribosome binding

Biological Process

• GO:0006417 regulation of translation
• GO:0008283 cell population proliferation
• GO:0032543 mitochondrial translation
• GO:0070131 positive regulation of mitochondrial translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005763 mitochondrial small ribosomal subunit
• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7a5f, PDBe:7a5f, PDBj:7a5f
• PDBsum: 7a5f
• PubMed: 33243891
• UniProt: Q92552|RT27_HUMAN Small ribosomal subunit protein mS27 (Gene Name=MRPS27)