Structure of PDB 7uck Chain V Binding Site BS01

Receptor Information

>7uck Chain V (length=131) Species: 9986 (Oryctolagus cuniculus)

SGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGD
MVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNK
GEMKGSAITGPVAKECADLWPRIASNAGSIA

Ligand information

>7uck Chain 5 (length=3546)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggccgguccggccgugccggcggggcgga
ucucccgcucccgucggcgggggaccgccccccggccggcgaccggccgc
cgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacg
gcugggaaggccccggcggggaagguggcccgggggccccggguguuaca
gccccgccccggcagcagcagcucgccgaaucccggggccgagggagcga
gacccgugccgccgcgcucuccccccugggggggccgggccaccccuccc
acggcgcgaccgcuggggcggacuguccagugcgccccgggcgggucgcg
ccgucgggcccgggggccacggcgucccccgcgagcgcacggggucggcg
gcgaugucggcuacccacccgacccgucuugaaacacggaccaaggaguc
uaacacgugcgcgagucgggggcuacgaaagccgccguggcgcaaugaag
gugaaggccggcggcgccgccggccgaggugggaucccgaggccuuccag
uccgccgagggcgcaccaccggcccgucucgcccgccgcgccggggaggu
ggagcacgagcgcacguguuaggacccgaaagauggugaacuaugccugg
gcagggcgaagccagaggaaacucugguggagguccguagcgguccugac
gugcgaaucggucguccgaccuggguauaggggcgaaagacuaaucgaac
caucuaguagcugguucccuccaaaguuucccucaggauagcuggcgcuc
uccgcaguuuuauccgguaaagcgaaugauuagaggucuuggggccgaaa
cgaucucaaccuauucucaaacuuuaaauggguaagaagcccggcucgcu
gguguggagccgggcguggaaugcgagugccuagugggccacuuuuggua
agcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgcccga
ugccgacgcucaucagaccccagaaaagguguugguugauauagacagca
ggaugguggccauggaagucggaauccgcuaaggaguguguaacaacuca
ccugccgaaucaacuagcccugaaaauggauggcgcuggagcgucgggcc
cauacccggccgucgcuggcagucggcagagcgagaaagagaguggcgcg
gacgcuacgccgcgacgaguaggagggccgcugcggugagccuugaagcc
uagggcgcgggcccggguggagccgccgcaggugcagaucuuggugguag
uagcaaauauucaaacgagaacuuugaaggccgaaguggagaaggguucc
augugaacagcaguugaacaugggucagucgguccugagagaugggcgag
cgccguuccgaagggacgggcgauggccuccguugcccucagccgaucga
aagggagucggguucagauccccgaauccggaguggcggagaugggcgcc
gcgaggcguccagugcgguaacgcgaccgaucccggagaagccggcggga
gccccggggagaguucucuuuucuuugugaagggcagggcgcccuggaau
ggguucgccccgagagaggggcccgugccuuggaaagcgucgcgguuccg
gcggcguccggugagcucucgcuggcccuugaaaauccgggggagagggu
guaaaucucgcgccgggccguacccauauccgcagcaggucuccaaggug
aacagccucuggcauguuggaacaauguagguaagggaagucggcaagcc
ggauccguaacuucgggauaaggauuggcucuaagggcuggcagccgacu
uagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaagca
ucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccagugc
ucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcggga
guaacuaugacucucuuaagguagccaaaugccucgucaucuaauuagug
acgcgcaugaauggaugaacgagauucccacugucccuaccuacuaucca
gcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaagaa
gacccuguugagcuugacucuagucuggcacggugaagagacaugaucgu
uuuuucacugacccggugaggcgggggggcgagccgggcucucgcuucug
gcgccaagcgcccggccggccgggcgcgacccgcuccggggacagugcca
gguggggaguuugacuggggcgguacaccugucaaacgguaacgcaggug
uccuaaggcgagcucagggaggacagaaaccucccguggagcagaagggc
aaaagcucgcuugaucuugauuuucaguacgaauacagaccgugaaagcg
gggccucacgauccuucugaccuuuuggguuuuaagcaggaggugucaga
aaaguuaccacagggauaacuggcuuguggcggccaagcguucauagcga
cgucgcuuuuugauccuucgaugucggcucuuccuaucauugugaagcag
aauucaccaagcguuggauuguucacccacuaauagggaacgugagcugg
guuuagaccgucgugagacagguuaguuuuacccuacugaugauguguug
uugccaugguaauccugcucaguacgagaggaaccgcagguucagacauu
ugguguaugugcuuggcugaggagccaauggggcgaagcuaccaucugug
ggauuaugacugaacgccucuaagucagaaucccgcccaggcggaacgau
agcgccggagccucgguuggccucggauagccggucccccgccggggucc
ggugcggagagcccuucgucccgggaaacggggcggccggaaagggggcc
gcccucucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaa
ccauucguagacgaccugcuucugggucgggguuucguacguagcagagc
agcugcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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>>>>>>..[[[..<<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>>..<<<<<<<<<....<<<<<<<<..<<<<<<<<....<<<<..
....>>>>.....((......<<<.....))>>>...>.>>>>>>>...>
>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>..
...(((((..<<<<<<)))))..]]]........................
..........<<....>>...............<<<<....>>>>.....
.............................<<<<<<<<..<<<.<<...<<
<<<<<<.<<<.....<<<<<<<<<....<<<<<<<.<<<<<.<<<<<<<.
<<<<<<<<<......>>>>>>>>>>>>>>>>>>>>>>>>>...>>>..>>
>>>>>>>......>>>.>>>.>>>>>...<<<<<<<<<<<<<<<<<<<<<
<<...<<..<<<..<<<<<<<......<<<<<<<<.>>>>>>>>......
.>>>>>>>..>>>.........>>>>>>..>>>>>>>>>>>.....<<..
...{.>>......<<<<<<.<<<<<<..>>>>>>.<<<<<<..<<<.<<<
<<<<<<<<<<<<<<<<<<<<.<<<<..>>>>.>>>>>>.>>>.>>>>>>>
>>>>.>>>...>>>>>>>.>>...........>>>>>>.....>>>.>>>
>>...>>>>>...>>>>>>>>..<<<<........>>>><<<<<<....<
<<<<<<<<<<<<..<<<...<<<<.....>>>>.....>>>...<<....
>>...<<<<<<<<....>>>>>>>>..<<<<<...<<<..<<<...(((.
[[.>>>..>>>....>>>>>.<<<<<<<<<.<<<<......>>>>>>>>>
>>.>>....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<
<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.
.......>>>>.>>>....>>>>>>>>>...<<.......>>......>>
>>>>......>.>>..<<<<<<....<.<<<....>>>.><<.<<<<<<<
...<<<.<<<<<<<<<...<<<....<<<..<.<<<..<<<<.<......
...>.>>>>>>>.>..>>>..>.>>...>>>>>>>...<<<<<<<<<.<.
.....>>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<<<<<...
..>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(((...
....<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<
<<.<<.....................>>.........<<......>>...
>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>..>>>
...>>>>>>><<<<<<.<<<........}...........]].)))....
........>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<......<<<
<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<...
..<<.....<<<<<<.......>>>>>>.<<<.....<<<<<.<<...<<
<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<...
.>>>..........<<.<<<<<<<<<...>>>>>>>>>>>......>>..
..>>>..<<<<<........>>>>>))<<<<....<<<<<..<<<<<<<<
....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<<<<<<<<<
<<<<<<<<<<<<<..{{{..>>>>>>....<<<<<.<<<<.<<<<.....
<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<.......
>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>>>>>>..>.....
.....>>.>>>.>>>>.>>>..>>.....<<......>>>>>>>.<<<<.
....>>>>..>>>....<<<<.....<<<<<<<.<<.........<....
.<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<.>>>>>..>>
>>>>.>>............<<<<<<<<<..<<<.........>>>..<<<
<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<
<<.....<<.....<<<<....>>>>.....>>..>>>>..>>>>><<<<
<<.......>>>>>>........<...........<<<<<.......>>>
>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>
>>....>>....<<<<<........>>>>>....[[[[[..{{{.{{...
...[[[[.{{[[[[..<<<<<.<<<<<<<<<.<<<<<<<<<<......>>
>>>>>>>>...<<<<<.....<<<<..<<.<.<<<>>.>>.>>.......
.......<<<<<<<<<..>>>>>>>>>..>>>>..>>>>>....>>>>>>
>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>>>.
.<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>
....>>>>>>>...<<<..<<<<.......>>>>..>>>.<<<....>>>
.>>)))]......((((((..(((..>>>>>>.)))))))))........
..]]]]..}}]]]].......<<.<.<.<<<<<<...<<.......>>..
.>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<.<<<<<....
..>>>>>...>.>.<<<.........>>>.....>>>>>..>...>>>.>
>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]].....<<<<
<<.<..<<<..<.<<<<<.................>>>>>.><<<<....
<<<<....<<.<<<<<<<}}}.>>>>>..>>.>>...>>>>...>>>><<
<<<...<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>
.<<<<<<<..<<<<....<<<........<<<<<..<<<<<.>>>>>.>>
>>>.......>>>...<<<..<<<<....<<<<<<<<<........>>>>
>>.>>>...>>>>..>>>..............>>>>..>>.>>>>>.<<<
<<.<.............>....<<.<<<<<<<.<<.....<<<<.<..<<
<<<.>>>>>..>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7uck Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.
• Resolution: 2.8 Å
• Binding residue (original residue number in PDB): F14 R15 S17 G19 A24 V25 I40 S41 K43 G44 K46 R48 L49 N50 R51 L52 T64 K74 R85 K86 R89 F95
• Binding residue (residue number reindexed from 1): F5 R6 S8 G10 A15 V16 I31 S32 K34 G35 K37 R39 L40 N41 R42 L43 T55 K65 R76 K77 R80 F86

Gene Ontology

Molecular Function

• GO:0001223 transcription coactivator binding
• GO:0003735 structural constituent of ribosome
• GO:0031625 ubiquitin protein ligase binding
• GO:0070180 large ribosomal subunit rRNA binding
• GO:1990948 ubiquitin ligase inhibitor activity

Biological Process

• GO:0000122 negative regulation of transcription by RNA polymerase II
• GO:0006412 translation
• GO:0008284 positive regulation of cell population proliferation
• GO:0010628 positive regulation of gene expression
• GO:0032986 protein-DNA complex disassembly
• GO:0050821 protein stabilization
• GO:0070314 G1 to G0 transition
• GO:0072717 cellular response to actinomycin D
• GO:1901798 positive regulation of signal transduction by p53 class mediator
• GO:1903450 regulation of G1 to G0 transition
• GO:1904667 negative regulation of ubiquitin protein ligase activity
• GO:2000059 negative regulation of ubiquitin-dependent protein catabolic process

Cellular Component

• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0032991 protein-containing complex
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7uck, PDBe:7uck, PDBj:7uck
• PDBsum: 7uck
• PubMed: 35679869
• UniProt: G1T6D1|RL23_RABIT Large ribosomal subunit protein uL14 (Gene Name=RPL23)