Structure of PDB 6ppf Chain V Binding Site BS01

Receptor Information

>6ppf Chain V (length=73) Species: 1423 (Bacillus subtilis)

NGRDSEAKRLGAKRADGQFVTGGSILYRQRGTKIYPGENVGRGGDDTLFA
KIDGTVKFERFGRDRKKVSVYPV

Ligand information

>6ppf Chain A (length=2471)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuuga
uccggagauuuccgaauggggaaacccaccacucguaauggagugguauc
cauaucugaauucauaggauaugagaaggcagacccggggaacugaaaca
ucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcggc
gagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugua
ggacacucuguacggaguuacaaaggaacgagguagaugaagaggucugg
aaaggcccgccauaggagguaacagcccuguagucaaaacuucguucucu
ccugaguggauccugaguacggcggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaagag
auccugaaaccgugugccuacaaguagucagagcccguuaacgggugaug
gcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguua
agcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcauga
guacguggucguagacccgaaaccaggugaucuacccauguccaggguga
aguucagguaacacugaauggaggcccgaacccacgcacguugaaaagug
cggggaugagguguggguaggggugaaaugccaaucgaaccuggagauag
cugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuug
gagguagagcacugauuggacuagaauucagucaaacuccgaaugccaau
gacuuauccuugggagucagacugcgagugauaagauccguagucgaaag
ggaaacagcccagaccgccagcuaaggucccaaaguauacguuaagugga
aaaggauguggaguugcuuagacaaccaggauguuggcauuuaaagagug
cguaauagcucacuggucgagugacucugcgccgaguaccggggcuaaac
guaucaccgaagcugcggacuguucgaacagugguaggagagcguucuaa
gggcugugaagccagaccggaaggacugguggagcgcuuagaagugagaa
ugccgguaugaguagcgaaagaggggugagaauccccuccaccgaaugcc
uaagguuuccugaggaaggcucguccgcucaggguuagucgggaccuaag
ccgaggccgaaaggcguaggcgauggacaacagguugauauuccuguacc
accuccucaccauuugagcaauggggggacgcaggaggauaggguaagcg
cgguauuggauauccgcguccaagcaguuaggcugggaaauaggcaaauc
cguuucccauaaggcugagcugugauggcgagcgaaauuaguagcgaagu
uccugauuccacacugccaagaaaagccucuagcgaggugagaggugccc
guaccgcaaaccgacacagguaggcgaggagagaauccuaaggugaucga
gagaacucucguuaaggaacucggcaaaaugaccccguaacuucgggaga
aggggugcucuguuagggugcaagcccgagagagccgcagugaauaggcc
caggcgacuguuaaacacaggucucugcgaagccguaaggcgaaguauag
gggcugacgccuggcgcaacgaucugggcacugucucaacgagagacucg
gugaaauuauaguaccugugaaugcagguuacccgcgacaggacggaacc
ccguggagcuuuacugcagccugauauugaaugccuucuaacccgccgcc
cuuaucgggcggggagacagugucaggugggcaguuugacgggcggucgc
cuccuaaaagguaacggaggcgcccaaagguucccucagaaugguuggaa
aucauucgcagaguguaaaggcacaagggagcuugacugcgagaccuaca
agucgagcagggacgaaagucgggcuuagugauccggugguuccgcaugg
aagggccaucgcucaacggauaaaagcuaccccgggcuauccgucgcggg
cgcaggaaauuugagaggagcuguccuuaguacgagaggaccgggaugga
cgcaccgcugguguaccaguuguucugccaagggcaucgcuggguagcua
ugugcggacgggauaagugcugaaagcaucuaagcaugaagccccccuca
agaugagauuucccauuccgcaaggaaguaagaucccugaaagaugauca
gguugauaggucugagguggaaguguggcgacacauggagcugacagaua
cuaaucgaucgaggacuuaac

<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<.....
.<<<<<<..[..((.>>>.....<<<<<<<..<<<))..]..>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<....
>>......>>>>>.>.........<<....>>...<<<<......<<...
..>>.....>>>>...<<<....<<<<<<<<....>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>>
>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......
<<<<<..((((>>>>>..>>>......>>.>>>>>>>.>>..........
.....<<....>>....>>.........<<<<....>>>>..........
........>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<..
.<<<.....>>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..
>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<...
.......<....<<.<..............>.>>...>............
>>>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>....<
<<......>>>...<<<<.<<<...<<<(((.....<<<<<<........
...<<.<.<<<<<<..........<<<<<.<<<....>>>.........<
........>...>>>>>......>>>>>>.>>>.......))).....>>
>>>>.>>>...>>>>>>><<<<<<..>>>>>>....>>>>>><.<<<<<<
<.<<......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>....
.>>>>>><......<<....<<<<<<.......>>>>>><<<<....<<<
<<.<<<..<<<<<<...<<.....>>.>>>>>>....((<<<<.<<.<.<
<<...<<<....>>>...>>>..>>>....<<<<........>>>>.))<
<<<<.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.<<<
.<.....[....>.>>>.<...<....<.<<.<.<<<<<<<.<<.....>
>>>>>>>>....>.>>..>...>....><..<<..........>>...>>
.>>>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>>>>>
>.>>>.....<<......>>>>>>>.<<<.......>>>..>>>>..<<<
<....<<<<<<<<.<<.........<.....<<<<<....<<..>>....
.>>>>><<<<<....<<<<....>>>>...>>>>>............<<<
<<<<<<..<<<...>>>..<<<<<<<.....<<<....>>>.......>>
>>>>>..<<<...>>>...>>.>>>>>>>..>..>>.>>>>>>>>.>>>>
>>.....>.....<<<<<......>>>>>..<<<<<<<<.<<<......<
.<<.<<<<<<..<<<<<.<<<<<<<<..<<<....>>>....<<.<<<<<
.......>>>>>>>......>>>>>>>>..>>>>>..<<.......>><<
<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<......
.>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<.....
.>>>..>>>>.<<<....>>>.>>)))]......<<<<<<.<<<..))))
..>>>>>>>>>.............>>>>..>>>>>....>>>>>>>>>>>
....<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.
<<<<<...<<<<...<<<<<<<<<<..]...>>>>>..>>>>>...>>>>
.>>>>>...<<<....<<<<<...........>>>>>....>>>.>>>>.
......>>>>>>>..<<<<....>>>>....<<<<<<<<.........>>
>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.
>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6ppf Structural consequences of the interaction of RbgA with a 50S ribosomal subunit assembly intermediate.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): N20 D23 S24 E25 A26 R28 A34 D35 G36 Q37 G41 G42 S43 I44 R47 R49 G50 T51 K52 G62 G63 D64 F77
• Binding residue (residue number reindexed from 1): N1 D4 S5 E6 A7 R9 A15 D16 G17 Q18 G22 G23 S24 I25 R28 R30 G31 T32 K33 G43 G44 D45 F58

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6ppf, PDBe:6ppf, PDBj:6ppf
• PDBsum: 6ppf
• PubMed: 31665744
• UniProt: P05657|RL27_BACSU Large ribosomal subunit protein bL27 (Gene Name=rpmA)