Structure of PDB 5mc6 Chain V Binding Site BS01

Receptor Information
>5mc6 Chain V (length=188) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
GISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIHSVRTRGG
NKKYRALRIETGNFSWASEGISKKTRIAGVVYHPSNNELVRTNTLTKAAI
VQIDATPFRQWFEAHYGQTLGKKSKNAERKWAARAASAKIESSVESQFSA
GRLYACISSRPGQSGRCDGYILEGEELAFYLRRLTAKK
Ligand information
>5mc6 Chain 2 (length=1767) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccggucggauuuccaacggggccuuuccuucuggcuaaccuugagucc
uuguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguu
caaagcaggcguauugcucgaauauauuagcauggaauaauagaauagga
cguuugguucuauuuuguugguuucuaggaccaucguaaugauuaauagg
gacggucgggggcaucaguauucaauugucagaggugaaauucuuggauu
uauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaa
ucaagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucu
uaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugacc
cacucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguau
ggucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggag
uggagccugcggcuuaauuugacucaacacggggaaacucaccaggucca
gacacaauaaggauugacagauugagagcucuuucuugauuuugugggug
guggugcauggccguucuuaguugguggagugauuugucugcuuaauugc
gauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauuugcug
guuauccacuucuuagagggacuaucgguuucaagccgauggaaguuuga
ggcaauaacaggucugugaugcccuuagacguucugggccgcacgcgcgc
uacacugacggagccagcgagucuaaccuuggccgagaggucuugguaau
cuugugaaacuccgucgugcuggggauagagcauuguaauuauugcucuu
caacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacgu
cccugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuua
gugaggccucaggaucugcuuagagaucucagagcggagaauuuggacaa
acuuggucauuuagaggaacuaaaagucguaacaagguuuccguagguga
accugcggaaggaucau
...<<<<<.{.[[[[>>>>><<<<.<<<<<<...<.<...<.........
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..................<<....<<
.<<.......>>.>>.....>>.........<<<<<..<<....>>..>>
>>><...<<<<<<......(((((((.<.<....>.>.............
.>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>
>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.......
...>>>.>>...<<<..>>>.....>>>............<<<....<<<
.....>>>..>>>..>....>.>.....<<<<<<<<<.<<......>>.>
>>>>>.>>>......<<..<..<....>...>..>>.........<<<<<
((....<.<<<<.....<<..))>>.......>>>>>>>>>>..>>>>>>
>>>>.........<<<[[.....<.<<...<<<.<<....<<<<<<.<<<
<<.....<>.....>>>>>.>>>>>>.....<<<<<..............
.................>>>>>....<<<<<.<<.......<<...<...
....>..<<<.....>>>....>>......>>.>>..>>>.........<
<...<<<<<<<<.....))))))).>>>>>>>>..>>.....>>....<<
<<<<.<<...<<<<..<<<.<<<<<<.<...<<<......>>>......>
.>>>>>>.>>>.......<<....>>...>>>>..>.>>>>.>>>...>>
>...>>.>....<<<<<<<...<...<<<<.<.....>.>>>>...>>>>
>>>>..........<<<.<<.<<<..<<<<<<<<.<<<........>>>>
>>>>>>>..>>>...<<..]]>>...>>.....>>>.>>>.<<<......
<<<<....>>>>....>>>..]]]].}<<<<<.<<<<<<<..<<.<<<<<
<......<<<<<<......<<........>>..........<<<<<.<..
..<<<<<........<<.<<<........>>>.>>......>>>>>...<
<.<<<..<<<<<<<<<....<<<.<<<<<....>>>...<<<......>>
>...>>.>>>....<<<<<.<<..<<<<..<<<<<...............
.....>>>>>..>>>>.>>....<<<<<<.....>>>>>>.......>>>
>>....>>>.>>>...>.>>>>>>>.....>.>>>>>...>>.>>>>...
......<<<<<<.......<<.....<<..<<<<....>>>>..>>....
>>........>>>>>>...........<<<<<<..........>>>>>>.
.........>>>>>>....<<<<<<<<.......>>>>>>>>......>>
...>>>>>>>>>>.>>.......<<.<..<<<<.<<....<<<<<<<<.<
<<..<.<<..<...<<<<<<<..........>>>>>>>...>..>>.>..
>>>.>>>>>>>>...>>.>>>>...>.>>.........<<<<<<<<<<..
>>>>>>>>>>.......
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5mc6 The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex.
Resolution3.8 Å
Binding residue
(original residue number in PDB)
G2 I3 R5 R8 K10 R11 S12 A13 T14 A16 K17 R18 F21 R22 K23 K24 R25 K26 F27 L29 R31 Q32 P33 A34 K41 R42 I43 H44 R47 T48 R49 G50 N52 K54 R56 L58 R59 N64 I72 S73 K75 H84 P85 S86 N87 N88 E89 L90 V91 R92 K98 S136 N138 R141 K142 S170 S171 R172 G174 Q175 S176 R178 D180 A198 K200
Binding residue
(residue number reindexed from 1)
G1 I2 R4 R7 K9 R10 S11 A12 T13 A15 K16 R17 F20 R21 K22 K23 R24 K25 F26 L28 R30 Q31 P32 A33 K40 R41 I42 H43 R46 T47 R48 G49 N51 K53 R55 L57 R58 N63 I71 S72 K74 H83 P84 S85 N86 N87 E88 L89 V90 R91 K97 S124 N126 R129 K130 S158 S159 R160 G162 Q163 S164 R166 D168 A186 K188
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003735 structural constituent of ribosome
Biological Process
GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0022627 cytosolic small ribosomal subunit
GO:0030686 90S preribosome
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component
External links
PDB RCSB:5mc6, PDBe:5mc6, PDBj:5mc6
PDBsum5mc6
PubMed27980209
UniProtP0CX39|RS8A_YEAST Small ribosomal subunit protein eS8A (Gene Name=RPS8A)

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