Structure of PDB 3cc2 Chain V Binding Site BS01

Receptor Information

>3cc2 Chain V (length=65) Species: 2238 (Haloarcula marismortui)

TVLHVQEIRDMTPAEREAELDDLKTELLNARAVQAAGGAPENPGRIKELR
KAIARIKTIQGEEGD

Ligand information

>3cc2 Chain 0 (length=2754)

uaugccagcugguggauugcucggcucaggcgcugaugaaggacgugcca
agcugcgauaagcuguggggagccgcacggaggcgaagaaccacagauuu
ccgaaugagaaucucuaacaauugcuucgcgcaaugaggaaccccgagaa
cugaaacaucucaguaucgggaggaacagaaaacgcaacgugaugucguu
aguaaccgcgagugaacgcgauacagcccaaaccgaagcccucacgggca
auguggugucagggcuaccucucaucagccgaccgucuucacgaagucuc
uuggaauagagcgugauacagggugacaaccccguacugaagaccaguac
gcugugcgguagugccagaguagcggggguuggauaucccucgcgaauaa
cgcaggcaucgacugcgaaggcuaaacacaaccugagaccgauagugaac
aaguagugugaacgaacgcugcaaaguacccucagaagggaggcgaaaua
gagcaugaaaucaguuggcgaucgagcgacagggcauacaaggucccuug
acgaaugaccgagacgcgagucuccaguaagacucacgggaagccgaugu
ucugucguacguuuugaaaaacgagccagggagugugucuguauggcaag
ucuaaccggaguauccggggaggcacagggaaaccgacauggccgcaggg
cuugcccgagggccgccgucuucaagggcggggagccauguggacacgac
ccgaauccggacgaucuacgcauggacaagaugaagcgugccgaaaggca
cguggaagucuguuagaguugguguccuacaauacccucucgugaucuau
guguaggggugaaaggcccaucgaguccggcaacagcugguuccaaucga
aacaugucgaagcaugaccuccgccgagguagucugugagguagagcgac
cgauugguccugucaaacuccaaacuuacagacgcuguuugacgcgggga
uuccggugcgcgggguaagccuguguaccaggaggggaacaacccagaga
uagguuaagguccccaaguguggauuaaguguaauccucugaaggugguc
ucgagcccuagacagccgggaggugagcuuagaagcagcuacccucuaag
aaaagcguaacagcuuaccggccgagguuugaggcgcccaaaaugaucgg
gacucaaauccaccaccgagaccuguccguaccacucauacugguaaucg
aguagauuggcgcucuaauuggauggaagcaggggcgagagcuccugugg
accgauuagugacgaaaauccuggccauaguagcagcgauagucggguga
gaaccccgacggccuaauggauaaggguuccucagcacugcugaucagcu
gaggguuagccgguccuaagucucaccgcaacucgacugagacgaaaugg
gaaacagguuaauauuccugugccaucaugcagugaaaguugacgcccug
gggucgaucacgccgggcaucgcccggucgaaccguccaacuccguggaa
gccguaauggcaggaagcggacgaacggcggcauagggaaacgugauuca
accuggggcccaugaaaagacgagcaugauguccguaccgagaaccgaca
cagguguccauggcggcgaaagccaaggccugucgggagcaaccaacguu
agggaauucggcaaguuagucccguaccuucggaagaagggaugccugcu
ccggaacggagcaggucgcagugacucggaagcucggacugucuaguaac
aacauaggugaccgcaaauccgcaaggacucguacggucacugaauccug
cccagugcagguaucugaacaccucguacaagaggacgaaggaccuguca
acggcgggggucuuaagguagcguaguaccuugccgcaucaguagcggcu
ugcaugaauggauuaaccagagcuucacugucccaacguugggcccggug
aacuguacauuccagugcggagucuggagacacccagggggaagcgaaga
cccuauggagcuuuacugcaggcugucgcugaggacucucacuccgggag
gaggacaccgauagccgggcaguuugacuggggcgguacgcgcucgaaaa
gauaucgagcgcgcccuauggucaucucagccggggacccggcgaagagu
gcaagagcaaaagaugacuugacaguguucuucccaacgaggaacgcuga
cgcgaaagcguggucuagcgaaccaauuagccugcuugaugcgggcaauu
gaugacagaaaagcuacccuagggauaacagagucgucacucgcaagagc
acauaucgaccgaguggcuugcuaccucgaugucgguucccuccauccug
cccgugcagaagcgggcaagggugagguuguucgccuauuaaaggagguc
gugagcuggguuuagaccgucgugagacaggucggcugcuaucuacuggg
uguguaggugucugacaagaacgaccguauaguacgagaggaacuacggu
ugguggccacugguguaccgguuguucgagagagcacgugccggguagcc
acgccacacgggguaagagcugaacgcaucuaagcucgaaacccacuugg
aaaagagacaccgccgaggucccgcguacaagacgcggucgauagacucg
gggugugcgcgucgagguaacgagacguuaagcccacgagcacuaacaga
ccaa

..<<<<<<<<<<<.....((..((<<<<<<.[<<......<<<<<<.((.
.((.>>>.....<<<<<<...<<<))..)).>>>......>>>>>>...>
>>....<<<...>>>.....<<<<<.....>>>>>.>>...<<<<<<...
..<......>......>>>>>.>.........<<.....>>...<<<<<.
....<<.....>>....>>>>>...<<<...<<<...<<<<....>>>>.
....>>>....<<<<<<<<......<<<<.....<<<<<<<......<<<
<......>>>>.((......<<<.....))>>>....>>>>>>>......
>>>>...>>>>>>.>>......<<<<<<<........>>>>>>>......
<<<<<.<<<<<<>>>>>..>>>......]..>>>>>>.))..........
.....<<....>>....))..........<<<.....>>>..........
.........>>>>>>>>>>>.......<<<<<<<<<<....<.<<<<.<<
<.........<<<<<....>>>>>.........>>>.>>>>...>.>>>>
>>>>>>...<<<........>>>.<<<<<<...<<<<<<<<<<<<<<..<
<<...<<<<<...>>>>>...>>>...<<....>>....<<<<<...<<<
<..>>>>...>>>>><<<<<<....>>>>>>...>>>>>>>>>>>>>>.<
<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>
>>>.....>>>....<<<..<<.<<........>>>>.>>>....>>>>>
>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<..
..<<<<<....>>>>><.<<<<<<...<<<.<<<<<<<<<...<<<....
.<<..<<.>>..>>...>>.>...>>>>>>>>>>>>.......>>>>>>.
.>..<<<<<<.<<<.....>>>.>>>>>>....<<<......>>>...<<
<<<<<...<<<(((.....<<<<<<.....<<...........<<<.<<<
<<<<<<<.......<<<<<.<<...<<<<.......>>>>...>>.....
..<<<<......>>>>.>>>>>...>>>>>>>>>>>>>..........))
)>>....>>>>>>.>>>...>>>>>>><<<<<<<.......>>>>>..>>
....>>>>>><...<<<<<<<<......<<<<<<<<....>>>>>>>>..
.>>>>>>>>....>.....>>>>>><<.....<........<<<<<<...
....>>>>>><<<<<...<<<<..<<...<<<<<<...<<.....>>.>>
>>>>....((<<<<<<...<<<<<<.<<.....>>..>>>>>>.....>>
>..<<<<<........>>>>>))<<<<<<<<.......<<<...<<<<<<
<<...<<<<<<<<<<<<<.[[>>>>>>><...<<<<<....<<<<<....
<<<<...>>>>.....>>>>>.....>>>>>....>......>>>>>>..
.>>>>>>>>.........>>>..>>>>>>>>.>>>..>>.....<<....
..>>.>>>>..<<<.......>>>.>>>>>...<<<<.....<<<<<<<<
.<<.........<.....<<<<<...............>>>>><<<<<<<
<<.....>>>>>>>>>............<<<<<<<<<..<<<........
.>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<
<<..<<<<<<<..<<<.....<<<<......>>>>.....>>>>>>>>..
>>>>><<...>>........<...........<<<<<.......>>>>>.
....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>.>.
...>>.((.<<<<<<......))>>>>>>.<<<<<<<..(((.[[.....
.<<<<.[[<<<<..<<<<<.<<<<<<<<..<<....>>...<<<<....>
>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<.....
......>>>>>>..<<...<<<<<<<...<<<<<<..>>>>>>.....<<
...(((>>....>>>>>>>...<<<<<<<<<.........>>>>>>>>>.
<<<....>>>.>>))).......<<<<<.<<<<<.>>>>>>>>>>>>>>>
>..........>>>>..]]>>>>.......<<<<.<.<<<<<<...<<..
.....>>...>>>>>>...>>>>.>.......<<<<<..<<<<<..<<<<
<<<<<......>>>>>>>..>>..<<<<.....>>>>......>>>>>..
....>>>.>>....<<<<<<<....>>>.>>>>....]]..))).>>>>>
>>....<<<<<<...<<<<..<<<<<<<<...............>>>>>>
>><<<<<...<<<<...<<<<<<<<<<]]..>>>>>...>>>>>...>>>
>.>>>>>..<<<....<<<<<...........>>>>>....>>>.>>>>.
......>>>>>>.....<<<<<<<<<.......>>>>>....<<<.<<<<
<<<<....<<<<<..........>>>>>...>>>>.>>>>..>>>...>>
>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3cc2 Mutations outside the anisomycin-binding site can make ribosomes drug-resistant.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): T1 H4 R9 Q34 G44 R50 K51 A54 R55 K57 T58 E62
• Binding residue (residue number reindexed from 1): T1 H4 R9 Q34 G44 R50 K51 A54 R55 K57 T58 E62

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3cc2, PDBe:3cc2, PDBj:3cc2
• PDBsum: 3cc2
• PubMed: 18455733
• UniProt: P10971|RL29_HALMA Large ribosomal subunit protein uL29 (Gene Name=rpl29)