Structure of PDB 6zqf Chain UC Binding Site BS01

Receptor Information

>6zqf Chain UC (length=47) Species: 559292 (Saccharomyces cerevisiae S288C)

DGKRAINYQILKNKGLTPKRNKDNRNSRVKKRKKYQKAQKKLKSVRA

Ligand information

>6zqf Chain D3 (length=1392)

uaucugguugauccugccaguucauaugcuugucucaaagauuaagccau
gcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcucauu
aaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacugu
gguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagaga
uguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauucau
aauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaau
uucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuca
acggguaacggggaauaaggguucgauuccggagagggagccugagaaac
ggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaa
uucagggagguagugacaauaaauaacgauacagggcccauucgggucuu
guaauuggaaugaguacaauguaaauaccuuaacgaggaacaauuggagg
gcaagucuggugccagcagccgcgguaauuccagcuccaauagcguauau
uauguugcaguuaaaaagcucguaguugaacuuugggcccgguuggccgg
ucggauuuccaacggggccuuuccuucuggcuaaccuugaguccuugugg
cucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguucaaagc
aggcguauugcucgaauauauuagcauggaauaauagaauaggacguuug
guucuauuuuguugguuucuaggaccaucguaaugauuaauagggacggu
cgggggcaucaguauucaauugucagaggugaaauucuuggauuuauuga
agacuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaaga
acgaaaguuaggggaucgaagaugaucagauaccgucguagucuuaacca
uaaacuaugccgacuagggaucgggugguguuuuuuuaaugacccacucg
gcaccuuacgagaaaucaaagucuuuggguucuggggggaguauggucgc
aaggcuaaauuaaaggaauugacggaagggcaccaccaggaguggagccu
gcggcacgcgcgcuacacugacggagccagcgagucuaaccuuggccgag
aggucuugguaaucuugugaaacuccgucgugcuggggauagagcauugu
aauuauugcucuucaacgaggaauuccuaguaagcgcaagucaucagcuu
gcguugauuacgucccugcccuuuguacacaccgcccgucgcuaguaccg
gaacuaauuccguaggugaaccugcggaaggaucauuaaaga

...<<<<<.......>>>>>...<<<<<<...<.<..........<<<.<
<<..<<....<<....<<..........>>...>>.>>......<<....
....<<<..<<..<<....<<<..................<.....<<.<
<.......>>.>>......>.........<<<<<..<<....>>..>>>>
><...<<<<<<.........(((..<<<....>>>..............>
>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>>>
..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>....
..>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.........
.>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<..
...>>>..>>>.......>.>.....<<<<<..<.<..........>.>.
.>>.>>>......<<..<...........>..>>.........<<<<<<.
......<<<....>>>..................>>>>>>..>>>>>>..
.......<<<.[.....<.<<...<<<.<<....<<<<<<.<<<<<....
<..>....>>>>>.>>>>>>....<<<<<<....................
...........>>>>>>...<<<<<.<<.......<<...<.......>.
.<<<.....>>>....>>......>>.>>..>>>.........<<...<<
<<<<<<......)))....>>>>>>>>..>>.....>>....<<<<<<.<
<...<<<<..<<..<<<<<<.<...<<<......>>>......>.>>>>>
.>.>>......<<<....>>>..>>>>...>>>>>.>>>...>>>...>>
.>....<<<<<<<...<...<<<<.........>>>>...>>>>>>>>..
........<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>
>..>>>...<<..].>>...>>.....>>>.>>>................
....................<<<<<<<<<<<<..<<..<<<<<..<<<.<
<<.....>>>.>>>.....<<<<<<<......<<.....<<..<<<<...
.>>>>..>>....>>.......>>>>>>>...........<<<<<<....
......>>>>>>..........>>>>>.....<<<<<<<.........>>
>>>>>......>>...>>>>>>>............>>>>>..<<<<.<<>
>.>>>>.<<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6zqf 90 S pre-ribosome transformation into the primordial 40 S subunit.
• Resolution: 4.9 Å
• Binding residue (original residue number in PDB): K553 K556 R559 N560 V563 Q570 K574
• Binding residue (residue number reindexed from 1): K19 K22 R25 N26 V29 Q36 K40

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding
• GO:0042802 identical protein binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6zqf, PDBe:6zqf, PDBj:6zqf
• PDBsum: 6zqf
• PubMed: 32943521
• UniProt: Q12136|SAS10_YEAST Something about silencing protein 10 (Gene Name=SAS10)