Structure of PDB 7r81 Chain U1 Binding Site BS01

Receptor Information

>7r81 Chain U1 (length=173) Species: 5141 (Neurospora crassa)

GRLQEYQVIGRHLPTESNPTPALYRMRIFAPNAVVAKSRFWYFLRGLRKV
KKATGEIVTCNVITEKHPLKVKNFGVWIRYDSRSGTHNMYKEYRELSRTA
AVEALYSDMAARHRARFRSIHILKVVELEKTEDIKRPYLKQLVTKKLSFP
LPHRVPKISNKKIFSAKRPATFA

Ligand information

>7r81 Chain A1 (length=3123)

uugaccucggaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaacagggauugcccuaguaacggcgagugaagc
ggcaacagcucaaauuugaaaucuggcuucggcccgaguuguaauuugua
gaggaagcuuuuggugaggcaccuucugaguccccuggaacggggcgcca
uagagggugagagccccguauagucggaugccgauccaauguaaagcucc
uucgacgagucgaguaguuugggaaugcugcucaaaaugggagguaaauu
ucuucuaaagcuaaauaccggccagagaccgauagcgcacaaguagagug
aucgaaagaugaaaagcacuuugaaaagaggguuaaauagcacgugaaau
uguugaaagggaagcguuugugaccagacuugcgccguuccgaucauccg
guguucucaccggugcacucgggacggcucaggccagcaucgguuuuggc
ggggggauaaagguccggggaacguagcuccuccgggaguguuauagccc
cgggcguaaugcccucgccgggaccgagguucgcgcaucugcaaggaugc
uggcguaauggucaucaacgacccgucuugaaacacggaccaaggaguca
agguuuugcgcgaguguuuggguguaaaacccgcacgcguaaugaaagug
aacguaggugagagcuucggcgcaucaucgaccgauccugauguauucgg
auggauuugaguaagagcguuaagccuuggacccgaaagauggugaacua
ugcuuggauagggugaagccagaggaaacucugguggaggcucgcagcgg
uucugacgugcaaaucgaucgucaaaucugagcaugggggcgaaagacua
aucgaaccaucuaguagcugguuaccgccgaaguuucccucaggauagca
guguuguucuucaguuuuaugagguaaagcgaaugauuagggaccauucu
caaacuuuaaauauguaagaagcccuuguuacuuaauugaacgugggcau
ucgaauguaccaacacuagugggccauuuuugguaagcagaacuggcgau
gcgggaugaaccgaacgcgggguuaaggugccggaguggacgcucaucag
acaccacaaaaggcguuaguacaucuugacagcaggacgguggccaugga
agucggaauccgcuaaggacuguguaacaacucaccugccgaauguacua
gcccugaaaauggauggcgcucaagcgucccacccauaccccgcccucag
gguagaaacgaugcccugaggaguaggcggccguggaggucagugacgaa
gccuagggcgugagcccgggucgaacggccucuagugcagaucuuggugg
uaguagcaaauacuucaaugagaacuugaaggaccgaaguggggaaaggu
uccaugugaacagcgguuggacauggguuagucgauccuaagccauaggg
aaguuccguuucaaaggggcagugccccguguggcgaaagggaagccggu
uaacauuccggcaccuggauguggguuuugcgcggcaacgcaacugaacg
cggagacgacggcgggggccccgggcagaguucucuuuucuucuuaacgg
ucuaucacccugaaaacaguuugucuggagauaggguuuaauggccggaa
gagcccgacacuucugucggguccggugcgcucucgacgucccuugaaaa
uccgcgggagggaauaauucucacgccaggucguacucauaaccgcagca
ggucuccaaggugaacagccucugguugauagaacaauguagauaaggga
agucggcaaaauagauccguaacuucgggaaaaggauuggcucuaagggu
uggacaaccaacuuagaacugguacggacagggggaaucugacugucuaa
uuaaaacauagcauugcgauggccagaaagugguguugacgcaaugugau
uucugcccagugcucugaaugucaaagugaagaaauucaaccaagcgcgg
guaaacggcgggaguaacuaugacucucuuaagguagccaaaugccucgu
caucuaauuagugacgcgcaugaauggauuaacgagauucccacuguccc
uaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauc
agcggggaaagaagacccuguugagcuugacucuaguuugacauugugaa
aauuuuuuuacuuauucaauuaagcggggcuggauuuucguccaacuucu
gguuuuaagguccuucgcgggccgacccggguugaagacauugucaggug
gggaguuuggcuggggcggcacaucuguuaaaccauaacgcagguguccu
aaggggggcucauggagaacagaaaucuccaguagaacaaaaggguaaaa
guccccuugauuuugauuuucagugugaauacaaaccaugaaaguguggc
cuaucgauccuuuagucccucgaaauuugaggcuagaggugccagaaaag
uuaccacagggauaacuggcuuguggcggccaagcguucauagcgacguc
gcuuuuugauccuucgaugucggcucuuccuaucauaccgaagcagaauu
cgguaagcguuggauuguucacccacuaauagggaacgugagcuggguuu
agaccgucgugagacagguuaguuuuacccuacugaugaccucaucgcaa
ugguaauugagcuuaguacgagaggaaccgcucauucagauaauugguuu
uugcgguuguccgaccgggcagugccgcgacgcuaccaucugcuggauaa
uggcugaacgccucuaagucagaauccaugccagaacgcgaugauaccuc
cagcacguuguagacguauaagaauaggcuccggccucguaucuuagcag
gcgauuccuccguuggccuugaaguggccagcgguaauucgcguauugua
auuuuaacacgcgcuggaucaaauccuuugcagacgacuuagaugugcga
aaggguccuguaagcaguagaguagccuuguuguuacgaucugcugaggg
uaagcccuccuucgccuagauuu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<..<<.......<<.<<<....>>>.>>.......>>....
<<<<.<<<<<.<<<...<<<<<<..<<....<<<<......>>>>.((..
....<<<.....))>>>....>>..>>.>>>>...>>>.....>>>>>.>
>>>........<<<<<<<........>>>>>>>.....<<<<<..(((((
(>>>>>..>>>..................................<<...
.>>...............<<<<....>>>>....................
..............<<<<.<<<<<<.....<<.<<<<<<<....<....<
<....>>....>........>>>.>>>>.>>.<<<<<<<.<<<<<<<<<<
<<<<<.....<.<<<<<<<<......<<<<<....>>>>>.......>>>
>>>>>....>.>>>>>>>>>>>>>>>........<<<..>>>.....>>>
>>>>.....>>>>>>.>>>>..<<<<........>>>><<<<<<....<<
<<<<<<.<<<<..<<<..<<<<<.....>>>>>..>>>...<<....>>.
......<<<<<..<<....>>...>>>>>.<<<<<<<<...<<....>>.
..>>>.>>>.>>....>>>>.>>>>>>>>..<<.<..<<<<<<<.....<
<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<.
.<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>.
.....>>>>>>...>..>.>>...<<<<<....<.<<<....>>>.><<<
<<<<<<.....<<.<<<<<<<.....<<<....<<<..<.<<.>>.>..>
>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.>>>>..
...>>>>...>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>...
.<<<......>>>...<<<<<<<...<<<(((.....<<<<<<......<
..........<<<.<<<<<<<<<<.......<<<<<.<<<...<<.....
....>>...>>>.........<........>...>>>>>...>>>>>>>>
>>>>>..........))).>....>>>>>>.>>>...>>>>>>><<<<<<
<<<.........>>>>>>>>>....>>>>>.<.<<<<<<<<.<<......
<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<
.....<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<..
.<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<<...<<<
....>>>........<<<<<<.>>>>>>....>>>....>>...<<<<<.
.......>>>>>))<<<<.<<<<.<<<....<<<....>>>......(<<
<<<<...<<<<.<<<<<<<<<<<<<.<<<<.......>>>>.><...<<<
<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>...
.><<<<<<<<....>>>>>>>>>>>>.>.>>>>>>>.>>>>.........
>>>>>>.........>>>...>>>>>>>>>>>>..>>.....<<......
>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<..
.......<.....<<<<<....<<..>>.....>>>>><<.<<<<<<<<<
<<..>>>>>..>>>>>>.>>............<<<<<<<<<..<<<....
.....>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>...
<<..<<<..<<<<<<....<<<....<<<<<....>>>>.>...>>>.>>
>>..>>>>><<<<<<.......>>>>>>........<...........<<
<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>..>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<
<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<..<<<<<
<....>>>>>>...<<<<<.....<<<<..<<<<......>>>>......
....)...<<<<<.....>>>>>..>>>>..>>>>>.....>>>>>>>>.
.>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<<.
..<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>....
>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)
))......<<<<<<<..<<<<.))))))>>>>>>>>>>>.........>>
>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>>...>>>
>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>
>>>>>...>.<<<.........>>>.....>>>>>..>...>>>.>>...
.<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<<<<<<..
<<<<.<.<<<<<.................>>>>>.><<<<<...<<<<..
..<<<<<<<<<<....>>>>>..>>>>>...>>>>..>>>>>.<<<<...
<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>....<<
<<<<.<<<.<<......<<.......<<<....>>>....>>........
<<<<.....<<<<<<<<<.......>>>>>>>>>....>>>>........
......>>>>>.>>>>>>..<<.<.<<.............>>....<.<<
.<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>
....>>>>..>>..>...>.>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7r81 Structure of the translating Neurospora ribosome arrested by cycloheximide
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): G2 N62 T65 E66 K67 H68 P69 K71 S83 R84 S85 H88 M90 Y91 K92 A111 A112 R113 H114 R115 R117 R137 Y139 V144 T145 K146 P153 H154 N161 K163 A167 K168 A171 T172 F173
• Binding residue (residue number reindexed from 1): G1 N61 T64 E65 K66 H67 P68 K70 S82 R83 S84 H87 M89 Y90 K91 A110 A111 R112 H113 R114 R116 R136 Y138 V143 T144 K145 P152 H153 N160 K162 A166 K167 A170 T171 F172

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7r81, PDBe:7r81, PDBj:7r81
• PDBsum: 7r81
• PubMed: 34815343
• UniProt: V5IQT8