Structure of PDB 7pwo Chain U1 Binding Site BS01

Receptor Information

>7pwo Chain U1 (length=74) Species: 184922 (Giardia lamblia ATCC 50803)

KYTIIVVGKDLKLVERYVKSIVKEGRAPVRRLTITTRKSPCGEGTNTWHR
FELRMYKRTFWVTVVIPGIQVTVE

Ligand information

>7pwo Chain 2 (length=1355)

auccggucgauccugccggagcgcgacgcucuccccaaggacgaagccau
gcaugccucaggacgcggcggacggcucaggacaacgguugcaccccccg
uggcggucgccggacaccgcuggcaacccggcgccaagacgugcgcgcaa
gggcgggccccgggcgagcagcgugacgcagcgacggcccgcccgggcuu
ccggggcaucacccggucggcgcggucgcggcgcgccgagggcccgacgc
cuggcggagaaucaggguucgacuccggagagcgggccugcgagacggcc
cgcacauccaaggacggcagcaggcgcggaacuugcccaaugcgcggcgc
gcggcggcgcgacggggugcgcgcgagucaggcgggcccaccccgacccg
ccagggcaaggcuggugccagcagccgcgguaauuccagcucggcgagcg
ucgcgcggcgcugcugcaguugaaacgcccguaguuggccccccgccgcc
acgaggaaacgggagcgcuccaggcagguuggacccgccgcgugggaccg
cggcgggcccggcgcgccgcggcagccccgaggagagcgggcgggggcac
cgguaccggccggggacgggugaaacaggaugaucccgccgagaccgccg
gccgcgcaggcgccugccaagaccgccucugucaaucaagggcgaaggcc
gggggcuagaaggcgaucagacaccaccguauucccggccguaaacggug
ccgccccgcggccggcgcgcgucccgccggccgcccagggaaaccgggag
gcuccgggcucuggggggaguauggccgcaaggcugaaacuugaaggcau
ugacggagggguacaccagacguggagucugcggcucaaucugacucaac
gcgcgcaccucaccaggcccgcgcgguggugcauggccgcucccagcccg
uggcgcgagccgugcuccauugcgacaacgagcgagaccccggccgcggg
ccgcgggacggcccgcagcgggaggacggcggggcgauagcaggucugug
augcccuccgcccugggccgcacgcgcgcuacacuggcggggccagccgg
cgcccgcgaggacgcgagcccccgccguggccgggaccgcgggcuggaac
gcccccgcgcaccaggaaugucuuguaggcgcccgcccccaccgcgcgcc
ggacgcgucccugccccuuguacacaccgcccgucgcuccuaccgacugg
gcgcugcggcgagcgccccggacgggaagggccgcgagccccagcgccug
gaggaaggagaagucguaacaagguauccguaggugaaccugcggaugga
ucccu

..<<<<<.[..(((>>>>>.<<<<<<<<<..<<<....<......<<<.<
<<..<<<.........>>>......<<........<<<..<<..<<...<
<<<<......<<.....<<<<<<....>>>>>>......>>.....<<..
..<<<.......<.....>..>>>...>>.......>.>>>>..>>>>..
>>>.<<<....<<<..<<<<<<<<.......>>>>>>>>>>>......>>
>...<<<<<<<<....>>>>...>>>>.>>.<<<<<<..........>>>
>>>.<.<<....>>.>...>>>>>>.........<<<....<<<...>>>
..>>>..>.....>>>..........<<<.<<.........>>.>>>..<
<<.<(((...<<<<<.....<<.)))>>.......>>>>>>.>>>..>>>
>>>>>>..........<<<((.....<<<<...<<<.......<<<.<<<
<<<.......<<..<<.......>>........>>.....>>>>>..>.>
>><<<<<<.......>>>.>.>>..........<<<<<<<.....<<<<.
.<<..<<<<<.<<<..<.<......>.>.....>>>.>>>>.>.>>....
...<<....>>...>>>>....>>>>.>>>...>>>...>>>>...<<<<
<<<<...<...<<.<.<.....>.>.>>...>>>>>>>>>.........<
<<.<<..<<<<<<<<<<.<...>.>>>>>>>>>>...<<..))>>....>
>.....>>>.>>>.<<<......<<<<....>>>>....>>>..)))..]
<<<<<.<<<<<<<.<<.<<<<<<..<<<.<<<<<<......<<.......
.>>.........<<<<<.<.....<<.<<<..<<.<<<<<<....<<.<<
<<<<....>>>..<<......>>....>>>.>>....<<<<<.<<<<<<.
>>>>>>.....<<<<<.>>>>>.......>>>>>....>>>.>>>.....
>>>>>>>..>.>>>>>...>>.>>>>.>>>.....<<<<<<<.......<
<<.<<....>>.>>>....>>>>>>>......<....<<.<<<<......
>>>>.>>....>.....>>>>>>....<<<<<.<<.......>>.>>>>>
......>>...>>>>>>>>>>.>>....<..<<.<.<<<<<.<<....<<
<<<<<<.<<<..<<<.<<<.........>>>.>>>..>>>.>>>>>>>>.
..>>.>>>>>..>.>>...>.....<<<<<<<<<<..>>>>>>>>>>...
.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7pwo Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
• Resolution: 2.75 Å
• Binding residue (original residue number in PDB): L36 T69 R71 K72 S73 P74 G76 E77 G78 T79 W82 R84 Y90 R92
• Binding residue (residue number reindexed from 1): L11 T35 R37 K38 S39 P40 G42 E43 G44 T45 W48 R50 Y56 R58

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7pwo, PDBe:7pwo, PDBj:7pwo
• PDBsum: 7pwo
• PubMed: 35100413
• UniProt: A8B4G0