Structure of PDB 8phj Chain U Binding Site BS01

Receptor Information

>8phj Chain U (length=70) Species: 562 (Escherichia coli)

PVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKAS
AVKRHAKKLARENARRTRLY

Ligand information

>8phj Chain A (length=1503)

aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaaguuugcugacgaguggcggacgg
gugaguaaugucugggaaacugccugauggagggggauaacuacuggaaa
cgguagcuaauaccgcauaacgucgcaagaccaaagagggggccucuugc
caucggaugugcccagaugggauuagcuaguaggugggguaacggcucac
cuaggcgacgaucccuagcuggucugagaggaugaccagccacacuggaa
cugagacacgguccagacuccuacgggaggcagcaguggggaauauugca
caaugggcgcaagccugaugcagccaugccgcguguaugaagaaggccuu
cggguuguaaaguacuuucagcggggaggaagggaguaaaguuaauaccu
uugcucauugacguuacccgcagaagaagcaccggcuaacuccgugccag
cagccgcgguaauacggagggugcaagcguuaaucggaauuacugggcgu
aaagcgcacgcaggcgguuuguuaagucagaugugaaauccccgggcuca
accugggaacugcaucugauacuggcaagcuugagucucguagagggggg
uagaauuccagguguagcggugaaaugcguagagaucuggaggaauaccg
guggcgaaggcggcccccuggacgaagacugacgcucaggugcgaaagcg
uggggagcaaacaggauuagauacccugguaguccacgccguaaacgaug
ucgacuuggagguugugccggcguggcuuccggagcuaacgcguuaaguc
gaccgccuggggaguacggccgcaagguuaaaacucaaaugaauugacgg
gggcccgcacaagcgguggagcaugugguuuaauucgaugcaacgcgaag
aaccuuaccuggucuugacauccacggaaguuuucagagaugagaaugug
ccgaaccgugagacaggugcugcauggcugucgucagcucguguugugaa
auguuggguuaagucccgcaacgagcgcaacccuuauccuuuguugccag
cgguccggccgggaacucaaaggagacugccagugauaaacuggaggaag
guggggaugacgucaagucaucauggcccuuacgaccagggcuacacacg
ugcuacaauggcgcauacaaagagaagcgaccucgcgagagcaagcggac
cucauaaagugcgucguaguccggauuggagucugcaacucgacuccaug
aagucggaaucgcuaguaaucguggaucagaaugccacggugaauacguu
cccgggccuuguacacaccgcccgucacaccaugggaguggguugcaaaa
gaaguagguagcuuaaccuucgggagggcgcuuaccacuuugugauucau
gacuggggugaagucguaacaagguaaccguaggggaaccugcgguugga
uca

.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<...<<<<<<.>>>.>>>.>..>>>>>......
<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<.....
.>>>>>......>>.>.....<<<....>>>....<<<<<<.>>>>>>.>
>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<.........>>>>
>>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.<<<<<.
<.........>>>>>>.<<<<....>>>>...>>>>>>.........<<<
....<<<<....>>>>..>>>..>>.>>>>>>..<<<<......<<<<<.
.>>>>>.....>>>>...<.<<<<<......<.<<<<<<<<.......>>
>>>>>>.>........>>>>>....>..<<<<<(((...<<<<<.....<
<.)))>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((
.....<<<<...<<<.<<<<<<<.<<<<<<<<<<......<<<<<<....
.>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<
<....<<<<<<<<...<<<......>>>......>>>>>>>>........
...<<....>>.>>>>>>>..>>>>.>>>>...>>>...>>>>....<<<
<<<...<<...<<<<.<.....>.>>>>...>>>>>>>>..........<
<<<<<.<<<<<<<<<<<<..>>>>>>>>>>>>..<<..))>>.....>>>
>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<<.
<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>...
.......<<<<<<<......<<<<<<<..<<<<<<<....>>>>>>>...
.....>>>>>.>>.<<<.<<<..<<<<<<.......<<<<<<<<<....>
>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<<...<<..
<<<.....>>>>>....>>>>>>>.....<<<<<.....>>>>>......
..>>>>.........>>>...>>>>>>>>>...>>>>>>>...>>.>>>>
>>>>.....<<<<<<<.....<<<..<<...<<<....>>>...>>....
>>>.....>>>>>>>......<....<<<<<<<........>>>>>>>..
..>.....>>>>>>....<<<<<<<.........>>>>>>>......>>.
..>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<<<<<<<.
...<<<<<<.<<<<..<<....>>.>>>>>>>>>>...>>>>>>>>>>>>
..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>...
...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8phj Ribosomal stalk-captured CARF-RelE ribonuclease inhibits translation following CRISPR signaling
• Resolution: 3.67 Å
• Binding residue (original residue number in PDB): E31 R34 R35 K40 P41 T42 R45 K46 V53 H56 L70 Y71
• Binding residue (residue number reindexed from 1): E30 R33 R34 K39 P40 T41 R44 K45 V52 H55 L69 Y70

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8phj, PDBe:8phj, PDBj:8phj
• PDBsum: 8phj
• PubMed: 38033086
• UniProt: P68679|RS21_ECOLI Small ribosomal subunit protein bS21 (Gene Name=rpsU)