Structure of PDB 7u0h Chain U Binding Site BS01

Receptor Information

>7u0h Chain U (length=106) Species: 1247190 (Saccharomyces cerevisiae BY4741)

QKIAKTFTVDVSSPTENGVFDPASYAKYLIDHIKVEGAVGNLGNAVTVTE
DGTVVTVVSTAKFSGKYLKYLTKKYLKKNQLRDWIRFVSTKTNEYRLAFY
QVTPEE

Ligand information

>7u0h Chain 1 (length=2722)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagacaugguguuuug
ugcccucugcuccuuguggguaggggaaucucgcauuucacugggccagc
aucaguuuugguggcaggauaaauccauaggaauguagcuugccucggua
aguauuauagccugugggaauacugccagcugggacugaggacugcgacg
uaagucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacac
ggaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccauac
gcguaaugaaagugaacguagguuggggccucgcaagaggugcacaaucg
accgauccugaugucuucggauggauuugaguaagagcauagcuguuggg
acccgaaagauggugaacuaugccugaauagggugaagccagaggaaacu
cugguggaggcucguagcgguucugacgugcaaaucgaucgucgaauuug
gguauaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccg
aaguuucccucaggauagcagaagcucguaucaguuuuaugagguaaagc
gaaaacuuuaaauauguaaaaugaagagcuuuuagugggccauuuuuggu
aagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugccgg
aauacacgcucaucagacaccacaaaagguguuaguucaucuagacagcc
ggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuca
ccggccgaaugaacuagcccugaaaauggauggcgcucaagcguguuacc
uauacucuaccgucaggguuggaugcccugacgaguaggcaggcguggag
gucagugacgaagccuagaccguaaggucgggucgaacggccucuagugc
agaucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaa
guggggaaagguuccacgucaacagcaguuggacguggguuagucgaucc
uaagagauggggaagcuccguuucaaaggccugauuuuaugcaggccacc
aucgaaagggaauccgguuaagauuccggaaccuggauauggauucuuca
cgguaacguaacugaauguggagacgucggcgcgagcccugggaggaguu
aucuuuucuucuuaacagcuuaucaccccggaauugguuuauccggagau
ggggucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcu
ugugacggcccgugaaaauccacaggaaggaauaguuuucaugccagguc
guacugauaaccgcagcaggucuccaaggugaacagccucuaguugauag
aauaauguagauaagggaagucggcaaaauagauccguaacuucgggaua
aggauuggcucuaagggucggguagugagggccuuggucagacguagacg
gccuuggugucgcuugcuacaauuaacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
agugauguugacgcaaugugauuucgauuaacgagauucccacugucccu
aucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauca
gcggggcccuguugagcuuuuagucccucggaauuugaggcuagaaguua
ccacagggauaacuggcuuguggcagucaagcguucauagcgacauugcu
uuuugauugaugucggcucuuccuaucauaccgaagcagaauucgguaag
cguuggauuguucacccacuaauagggaagcuggaccgucgugagacagg
uuaguuuacccuacugaugaauguuaccgcaauaguaauugaacuuagua
cgagaggaacaguucauucggauaauugguuuuugcggcugucugaucag
gcauugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaag
ucagaauccaugcuagaacgcggugauuucuuugcuccacacaauauaga
uggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcuag
caacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugcaa
ugucauuuugcguggggauaaaucauuuguauacgacuuagauguacaac
gggguauuguaagcaguagaguagccuuguuguuacgaucugcugagauu
aagccuuuguugucugauuugu

..........................<<<<<<.....<<....>>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>......
.......<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>>..>>>>>>>..
..>>>>>>>>>>>........<<<<<<<.<....>.>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<......<<<<....<
<..<<<<<<.<........>>>>>>>......>>....>>>>..<<<<<<
<.<<<<<<<<<<<<<<<.....<<<<<<<<<....<.<<<<<......>>
>>>>......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<.
...>>>.....>>>>>>>.....>>>>>.>>>>>..<<<<.<....>.>>
>><<<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>
>>...<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.
<<<<<<<<...<<....>>...>>>>.>>.>>....>>>>>>.>>>>>>.
.<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>
>>>>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>
>>>>>...<<.......>>......>>>>>>>.....>.>>..<<<<<<.
...<.<<......>>.><<<<<<<<<<...<<<.<<<<<<..........
...............>>>>>>>>>.>>>>>>>>.>>..<<<<.<<<<<..
...>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((..
...<<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<
<<.<<<...<<.........>>...>>>.........<<......>>...
>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>
>..>>>>>>><<<<<<<<<.....>>>>>>>>>....>>>>>><.<<<<<
<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.
....>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<...
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<
...<<....<<<....>>>........<<<<<<........>>>>>>...
.>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<<....<
<<....>>>...<...<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..
[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>
>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>.>.>>>
>>>>.>>>>.........>>>>>>...>.....>>>>..>>>>>>>>>>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<
<.....<<<<<<<.<<...............<<<<<..............
.>>>>><<.<<<<<<<<<<<..<<<<<<<.....................
............>>>>>>>.......>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>....<..>....>>.>>>>>>>.....>>.>
>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<<
<..<<<<<<<<<.<<<<<<<<<...<<<<.))))))>>>>.>>>>>>>>.
>.>>>>>>..............<<<<<...<<.......>>...>>>>>.
........((....<<<..<<<<<..<.<<<<<<<......>>>>>>>..
.>.<<<.....))..>>>.....>>>>>.>>>.<<<<<<<....>>>.>>
>>.......>>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<.....
..........>>>>>>.><<<<<...<<<<....<<<<<<<<<<]]].>>
>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>
>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<...
.<<..........<<<<<<.....>>>>>>......>>.......<<<<<
<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>.........
....>>.>>>.>>>>>>..<<<<<<<.............>.....<<<<<
<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>.
...>>>>.>>>>>.>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7u0h Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
• Resolution: 2.76 Å
• Binding residue (original residue number in PDB): K42 E44 G45 K70 S72 K74 Y75 K77 Y78 K81 K82 K85 R90 R94 S97 T100 N101 Y103
• Binding residue (residue number reindexed from 1): K34 E36 G37 K62 S64 K66 Y67 K69 Y70 K73 K74 K77 R82 R86 S89 T92 N93 Y95

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7u0h, PDBe:7u0h, PDBj:7u0h
• PDBsum: 7u0h
• PubMed: 36482249
• UniProt: P05749|RL22A_YEAST Large ribosomal subunit protein eL22A (Gene Name=RPL22A)