Structure of PDB 7sae Chain U Binding Site BS01

Receptor Information

>7sae Chain U (length=100) Species: 1423 (Bacillus subtilis)

HVKKGDKVMVISGKDKGKQGTILAAFPKKDRVLVEGVNMVKKHSKPTQAN
PQGGISNQEAPIHVSNVMPLDPKTGEVTRVGYKVEDGKKVRVAKKSGQVL

Ligand information

>7sae Chain A (length=2434)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuuga
uccggagauuuccgaauggggaaacccaccacucguaauggagugguauc
cauaucugaauucauaggauaugagaaggcagacccggggaacugaaaca
ucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcggc
gagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugua
ggacacucuguacggaguuacaaaggaacgagguagaugaagaggucugg
aaaggcccgccauaggagguaacagcccuguagucaaaacuucguucucu
ccugaguggauccugaguacggcggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaagag
auccugaaaccgugugccuacaaguagucagagcccguuaacgggugaug
gcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguua
agcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcauga
guacguggucguagacccgaaaccaggugaucuacccauguccaggguga
aguucagguaacacugaauggaggcccgaacccacgcacguugaaaagug
cggggaugagguguggguaggggugaaaugccaaucgaaccuggagauag
cugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuug
gagguagagcacugauuggacuaggaauucagucaaacuccgaaugccaa
ugacuuauccuugggagucagacugcgagugauaagauccguagucgaaa
gggaaacagcccagaccgccagcuaaggucccaaaguauacguuaagugg
aaaaggauguggaguugacucugcgccgaaaauguaccggggcuaaacgu
aucaccgaagcugcggacuguucuucgaacagugguaggagagcguucua
agggcugugaagccagaccggaaggacugguggagcgcuuagaagugaga
augccgguaugaguagcgaaagaggggugagaauccccuccaccgaaugc
cuaagguuuccugaggaaggcucguccgcucaggguuagucgggaccuaa
gccgaggccgaaaggcguaggcgauggacaacagguugauauuccuguac
caccuccucaccauuugagcaauggggggacgcaggaggauaggguaagc
gcgguauuggauauccgcguccaagcaguuaggcugggaaauaggcaaau
ccguuucccauaagccugagcugugauggcgagcgaaauauaguagcgaa
guuccugauuccacacugccaagaaaagccucuagcgaggugagaggugc
ccguaccgcaaaccgacacagguaggcgaggagagaauccuaaggugauc
gagagaacucucguuaaggaacucggcaaaaugaccccguaacuucggga
gaaggggugcucuguuagggugcaagcccgagagagccgcagugaauagg
cccaggcgacuguuuagcaaaaacacaggucucugcgaagccguaaggcg
aaguauaggggcugacgccuaggcgcaacgaucugggcacugucucaacg
agagacucggugaaauuauaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccguggagcuuuacugcagccugauauugaaugucc
uucuaacccgccgcccuuaucgggcggggagacagugucaggugggcagu
uugacuggggcggucgccuccuaaaagguaacggaggcgcccaaagguuc
ccucagaaugguuggaaaucauucgcagaguguaaaggcacaagggagcu
ugacugcgagaccuacaagucgagcagggacgaaagucgggcuuagugau
ccggugguuccgcauggaagggccaucgcucaacggauaaaagcuacccc
gguccgucgcgggcgcaggaaauuugagaggagcuguccuuaguacgaga
ggaccgggauggacgcaccgcugguguaccaguuguucugccaagggcau
cgcuggguagcuaugugcggacgggauaagugcugaaagcaucuaagcau
gaagccccccucaagaugagauuucccauuccgcaaggaaguaagauccc
ugaaagaugaucagguugauaggucugagguggaaguguggcgacacaug
gagcugacagauacuaaucgaucgaggacuuaac

<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<<<<<.....
.<<<<<<.....((.>>>.....<<<<<<<..<<<)).....>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<.....
.>......>>>>>.>.........<<....>>...<<<<......<<...
..>>.....>>>>...<<<....<<<<<<<<<..>>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>>
>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......
<<<<<..((((>>>>>..>>>......>>.>>>>>>>.>>..........
.....<<....>>....>>.........<<<<....>>>>..........
...>....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<..
.<<<.....>>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>.>>>>>>>..<<.....<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......
..>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<
<.....<<<...<<<...<.........>...>>>..>>.>.....>>>>
.>>>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>....
<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.......
....<<...<<<<<<..>>>>>>..>>...........))).....>>>>
>>.>>>...>>>>>>><<<<<<<...>>>>>>>....>>>>>><.<<<<<
<<.<<......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>...
..>>>>>><......<<....<<<<<<.......>>>>>><<<<....<<
<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...
<<<...<<<....>>>...>>>...>>...<<<<<........>>>>>))
<<<<<.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.<<
<.<...[.[....>.>>>.<<..<....<.<<<<.<<<<<<<.<<.....
>>>>>>>>>....>>>>..>...>...>><..<<...........>>...
>>.>>>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>>>
>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<
<<<....<<<<<<<<.<<<..............<<<<<....<<..>>..
...>>>>><<<<<....<<<<....>>>>...>>>>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>>.
......>>>>>>>..<<<<..>>>>...>>.>>>>>>>....>>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.<<................<<<<<..<<<<..<<<<<<<<<.<<<.....
>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>...>>>>
..<<<.......>>><<<<<<...........>>>>>>..<<...<<<<<
.<<...<<<.<.......>.>>>......<<...(((>>....>>>>>>>
...<<<<..<<<......>>>..>>>>.<<<....>>>.>>)))......
.<<<<<<<.<<..))))..>>>>>>>>>.............>>>>...>.
...>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<..........
.....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.].]>>>>>>.
.>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>>>
....>>>.>>>>.......>>>>>>>..<<<<....>>>>....<<<<<<
<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>.
..>>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7sae RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): K4 K5 M10 K15 K42 K43 H44 S45 G54 S66 N67 R80 Y83 K90
• Binding residue (residue number reindexed from 1): K3 K4 M9 K14 K41 K42 H43 S44 G53 S65 N66 R79 Y82 K89

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0009295 nucleoid
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7sae, PDBe:7sae, PDBj:7sae
• PDBsum: 7sae
• PubMed: 35141754
• UniProt: P0CI78|RL24_BACSU Large ribosomal subunit protein uL24 (Gene Name=rplX)