Structure of PDB 7bl3 Chain U Binding Site BS01

Receptor Information

>7bl3 Chain U (length=102) Species: 83333 (Escherichia coli K-12)

AAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPA
LNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSE
TI

Ligand information

>7bl3 Chain A (length=2902)

guuguaagguuaagcgacuaagcguacacgguggaugcccuggcagucag
aggcgaugaaggacgugcuaaucugcgauaagcgucgguaaggugauaug
aaccguuauaaccggcgauuuccgaauggggaaacccaguguguuucgac
acacuaucauuaacugaauccauagguuaaugaggcgaaccgggggaacu
gaaacaucuaaguaccccgaggaaaagaaaucaaccgagauucccccagu
agcggcgagcgaacggggagcagcccagagccugaaucaguguguguguu
aguggaagcgucuggaaaggcgcgcgauacagggugacagccccguacac
aaaaaugcacaugcugugagcucgaugaguagggcgggacacgugguauc
cugucugaauauggggggaccauccuccaaggcuaaauacuccugacuga
ccgauagugaaccaguaccgugagggaaaggcgaaaagaaccccggcgag
gggagugaaaaagaaccugaaaccguguacguacaagcagugggagcacg
cuuaggcgugugacugcguaccuuuuguauaaugggucagcgacuuauau
ucuguagcaagguuaaccgaauaggggagccgaagggaaaccgagucuua
acugggcguuaaguugcaggguauagacccgaaacccggugaucuagcca
ugggcagguugaagguuggguaacacuaacuggaggaccgaaccgacuaa
uguugaaaaauuagcggaugacuuguggcugggggugaaaggccaaucaa
accgggagauagcugguucuccccgaaagcuauuuagguagcgccucgug
aauucaucuccggggguagagcacuguuucggcaagggggucacuuacca
acccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacg
gcgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcu
aaggucccaaagucaugguuaagugggaaacgaugugggaaggcccagac
agccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauag
cucacuggucgagucggccugcgcggaagauguaacggggcuaaaccaug
caccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguu
cuguaagccugcgaaggugugcugugaggcaugcuggagguaucagaagu
gcgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccg
gaagaccaaggguuccuguccaacguuaaucggggcagggugagucgacc
ccuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauucc
uguacuugguguuacugcgaaggggggacggagaaggcuauguuggccgg
gcgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaaucc
ggaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaa
caaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaau
cguaccccaaaccgacacagguggucagguagagaauaccaaggcgcuug
agagaacucgggugaaggaacuaggcaaaauggugccguaacuucgggag
aaggcacgcugauauguaggugaggucccucgcggauggagcugaaauca
gucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugug
caaacacgaaaguggacguauacggugugacgccugcccggugccggaag
guuaauugaugggguuagcgcaagcgaagcucuugaucgaagccccggua
aacggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgg
guaaguuccgaccugcacgaauggcguaaugauggccaggcugucuccac
ccgagacucagugaaauugaacucgcugugaagaugcaguguacccgcgg
caagacggaaagaccccgugaaccuuuacuauagcuugacacugaacauu
gagccuugauguguaggauaggugggaggcuuugaaguguggacgccagu
cugcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaa
cguugacccguaauccggguugcggacagugucugguggguaguuugacu
ggggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaau
ccuggucggacaucaggagguuagugcaauggcauaagccagcuugacug
cgagcgugacggcgcgagcaggugcgaaagcaggucauagugauccggug
guucugaauggaagggccaucgcucaacggauaaaagguacuccggggau
aacaggcugauaccgcccaagaguucauaucgacggcgguguuugucggc
ucaucacauccuggggcugaaguaggucccaaggguauggcuguucgcca
uuuaaagugguacgcgagcuggguuuagaacgucgugagacaguucgguc
ccuaucugccgugggcgcuggagaacugaggggggcugcuccuaguacga
gaggaccggaguggacgcaucacugguguucggguugucaugccaauggc
acugcccgguagcuaaaugcggaagagauaagugcugaaagcaucuaagc
acgaaacuugccccgagaugaguucucccugacccuuuaaggguccugaa
ggaacguugaagacgacgacguugauaggccggguguguaagcgcagcga
ugcguugagcuaaccgguacuaaugaaccgugaggcuuaaccuuacaacg
cc

<<<<<<<<<<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<
<<<<......<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..]
.>>>......>>>>>>>...>>>....<<<....>>><<<<<<<....>>
>>>>>.<<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....
<<....>>......>>>>>.>.........<<....>>..<<<<<<....
.<<.....>>....>>>>>>..<<<..<<<<......<<<<<<<<<<<<<
.<<<...<<<<<......>>>>>((......<<<.....))>>>...>>>
...>>>>>>>>>>>>>...>>>>........<<<<<<<<.........>>
>>>>>>......<<<<<..<<<<>>>>>..>>>......>>.>>>>>>>.
>>...............<<....>>....>>.........<<<<.....>
>>>.............>....>>>>>>>>>>>.......<<<<..<<<<<
......>>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<
<<<<<<<<..<<<...<<.....>>...>>>...<<....>>..<<<<..
...>>>>.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<
<<<<..<<<<...<<<<<<......>>>>>>...>.>>>...<<<.<<<<
<........>>>>>>>>....>>>>>>>>>...<<<.....>>>......
>>>>>>......>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<
<...<<<<<<<<<.....<<<...<<<.<<<<...<<<<<<.>>>>.>>.
..>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<
<<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<.
..<<<(((.....<<<<<<...........<<..<<<<<..<<.......
.<<<<<.<<...<<<.........>>>...>>........<<<......>
>>..>>>>>....>>..>>>>>.>>...........))).....>>>>>>
.>>>...>>>>>>><<<<<..<<....>>..>>>>>....>>>>>><.<<
<<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>..
>.....>>>>>><......<<.....<<<<<<.......>>>>>><<<<.
...<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<
<<...<<<...<<<....>>>...>>>...>>>..<<<<<........>>
>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<
<....(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>
>>>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>
>>...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>
>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<
<<....<<<<<<<<.<<...............<<<<<....<<..>>...
..>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>
>>............<<<<<<<<<..<<<.........>>>..<<<<<<<.
....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<
<<........<<<<<<.<<....>>..>>>>>>........>>>>>>>>.
.>>>>>.<<<<<.......>>>>>.........<...........<<<<<
<.....>>>>>>.....>....>>>>>..>>.>>>>>>>.....>>.>>>
>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<
<.<<<.<<.....<<<<<.<<<<<<..<<<<<.<<<.<<<<<.<<<<<<<
<<<<.<<<<...........<<<<<..<<<......<<<<<<<<....>>
>>>>>>...>>>............>>>>>..>>>>.>>>>>>>>>>>...
<<<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<.
......>>><<<<<<...........>>>>>>..<<...<<<<<<<...<
<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<
<..<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<
<<.<<..>>>>..>>>>>>>>>.............>>>>..>>>>>>>..
...........<<<<<<...<<.......>>...>>>>>>.....<<<<<
..<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>
>.....>>>>>......>>>.>>....<<<<<<<....>>>.>>>>....
>>..>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<........
.......>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))).>>>>>
>..>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>
>>....>>>.>>>>.......>>>>>>>..<<<<<.....>>>>><....
<<..<<<<<.......>>>>>....<<<<<<<<..<<....<<<<<....
>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>>>>>>>>>.
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bl3 Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): K3 R5 G15 K16 V27 K32 K42 K43 H44 Q45 P47 G56 Q65 S67 N68 R81 F84 K90 K91
• Binding residue (residue number reindexed from 1): K3 R5 G15 K16 V27 K32 K42 K43 H44 Q45 P47 G56 Q65 S67 N68 R81 F84 K90 K91

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7bl3, PDBe:7bl3, PDBj:7bl3
• PDBsum: 7bl3
• PubMed: 33639093
• UniProt: P60624|RL24_ECOLI Large ribosomal subunit protein uL24 (Gene Name=rplX)