Structure of PDB 6yef Chain U Binding Site BS01

Receptor Information

>6yef Chain U (length=100) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGSVKVGAT
VEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA

Ligand information

>6yef Chain A (length=2881)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaaguuguugggguuguaggaca
cucggaguuacaaaggacgacauuagacgaaucaucuggaaagaugaauc
aaagaagguaauaauccuguagucgaaaauguugucucucuugaguggau
ccugaguacgacggagcacgugaaauuccgucggaaucugggaggaccau
cuccuaaggcuaaauacucucuagugaccgauagugaaccaguaccguga
gggaaaggugaaaagcaccccggaaggggagugaaauagaaccugaaacc
gugugcuuacaaguagucagagcccguuaaugggugauggcgugccuuuu
guagaaugaaccggcgaguuacgauuugaugcaagguuaagcaguaaaug
uggagccguagcgaaagcgagucugaauagggcguuuaguauuuggucgu
agacccgaaaccaggugaucuacccuuggucagguugaaguucagguaac
acugaauggaggaccgaaccgacuuacguugaaaagugagcggaugaacu
gaggguagcggagaaauuccaaucgaaccuggagauagcugguucucucc
gaaauagcuuuagggcuagccucaagugaugauuauuggagguagagcac
uguuuggacgaggggccccucggguaccgaauucagacaaacuccgaaug
ccaauuaauuuaacuugggagucagaacaugggugauaagguccguguuc
gaaagggaaacagcccagaccaccagcuaaggucccaaaauauauguuaa
guggaaaaggauguggcguugcccagacaacuaggauguuggcuuagaag
cagccaucauuuaaagagugcguaauagcucacuagucgagugacacugc
gccgaaaauguaccggggcuaaacauauuaccgaagcuguggauuguccu
ggacaaugguaggagagcguucuaagggcguugaagcaugaucguaagga
cauguggagcgcuuagaagugagaaugccggugugaguagcgaaagacgg
gugagaaucccguccaccgauugacuaagguuuccagaggaaggcucguc
cgcucuggguuagucggguccuaagcugaggccgacaggcguaggcgaug
gauaacagguugauauuccuguaccaccuauaaaucgauggggggacgca
guaggauaggcgaagcgugcgauuggauugcacgucuaagcaguaaggcu
gaguauuaggcaaauccgguacucguuaaggcugagcugugauggggaga
aguucgagucguugauuucacacugccgagaaaagccucuagauagaaaa
uaggugcccguaccgcaaaccgacacagguagucaagaugagaauucuaa
ggugagcgagcgaacucucguuaaggaacucggcaaaaugaccccguaac
uucgggagaaggggugcucuuuaggguuaacgcccagaagagccgcagug
aauaggcccaagcgacuguuuaucaaaaacacaggucucugcuaaaccgu
aaggugauguauaggggcugacgccugcccggugcuggaagguuaagagg
agugguuagcuucugcgaagcuacgaaucgaagccccaguaaacggcggc
cguaacuauaacgguccuaagguagcgaaauuccuugucggguaaguucc
gacccgcacgaaaggcguaacgauuugggcacugucucaacgagagacuc
ggugaaaucauaguaccugugaagaugcagguuacccgcgacaggacgga
aagaccccguggagcuuuacuguagccugauauugaaauucggcacagcu
uguacaggauagguaggagccuuugaaacgugagcgcuagcuuacgugga
ggcgcuggugggauacuacccuagcuguguuggcuuucuaacccgcacca
cuuaucguggugggagacagugucaggcgggcaguuugacuggggcgguc
gccuccuaaaagguaacggaggcgcucaaagguucccucagaaugguugg
aucauucauagaguguaaaggcauaagggagcuugacugcgagaccuaca
agucgagcagggucgaaagacggacuuagugauccggugguuccgcaugg
aagggccaucgcucaacggauaaaagcuaccccggggauaacaggcuuau
cucccccaagaguucacaucgacggggagguuuggcaccucgaugucggc
ucaucgcauccuggggcuguagucggucccaaggguugggcuguucgccc
auuaaagcgguacgcgagcuggguucagaacgucgugagacaguucgguc
ccuauccgucgugggcguaggaaauuugagaggagcuguccuuaguacga
gaggaccgggauggacauaccucugguguaccaguugucgugccaacggc
auagcuggguagcuauguguggacgggauaagugcugaaagcaucuaagc
augaagccccccucaagaugagauuucccaacugguuauaagaucccuca
aagaugaugagguuaauagguucgagguggaagcauggugacauguggag
cugacgaauacuaaucgaucgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<.>>>>>>>......<<<<<<
<<<<<<<.......<<<<<<<<<<.<<<...<<<<<......>>>>>((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>.>>>>>..>
>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<<<<
>>>>>..)))......]].>>>>>>>.]]...............<<....
>>....]].........<<<<....>>>>..................>>>
>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<...
.....>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<<.....>>
>...>>>...<<....>>..<<<<.....>>>>.....>>>>>.>>>>>>
>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<.....
.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>>>
>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<
....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<...
<<<<<<<<...<<...<.....>...>>...>>>>>>>>..>>.>.....
>>>>>.>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>
....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<...
........<<..<<<<.<<<<........<<.....<...<<........
...>>...>..........................>>....>>>>.>>>>
.>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<<.
>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<.<<....>>.
>>>>>...>>.>>>>>>>..>.....>>>>>><......<<....<<<<<
<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>
>.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>...>>>..>>
>...<<<<<........>>>>>))<<<<<<<..<<<...<<<<....<<<
<..<<...<<<<<.<.<<<.....{...>>>.>.<....<<<<...<<<.
<<<<<<..<<.....>>.>>>>>>......>>>...>>>>.....>....
........>>>>>....>>...>>>>.........>>>>..>>>.....>
>>>>>>>>>>..>>.....<<......>>>>>>>.<<<.......>>>..
>>>>..<<<<....<<<<<<<<.<<.........<.....<<<<<....<
<..>>.....>>>>><<<<<<<.<<<<....>>>>.>>>>>>>.......
.....<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<..
..>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<...<<..
.<<<<<<.<<....>>..>>>>>>...>>...>>>>>>>>..>>>>><<<
<<<.......>>>>>>........<...........<<<<<.......>>
>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>
>>>.....>.....<<<<<........>>>>>..<<<<<<<<.(((.[[.
.....<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<<.<<<...<.<<<
...........<<<<<..<<<......<<<.<<<......>>>.>>>...
>>>............>>>>>..>>>.>...>>>.>>>>....<<.<<<<<
<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>>
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
.>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<.....
.>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>
..))>>>>>>>.............>>>>..]]>>>>.......<<<<.<.
<<<<<<...<<.......>>...>>>>>>...>>>>.>.......<<<<<
.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>
>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....
]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<........
.......>>>>>>>.<<<<<...<<<<...<<<<<<<<<<..}...>>>>
>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<...........>>>
>>...>>>>.>>>>.......>>>>>>>.<<<..>>>....<<<<<<<<<
.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>
>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6yef Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 angstrom resolution.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): G9 K10 F21 E23 K24 K64 R67 K70 R78 R79 K80 R84 K85 K86 G87 H88 R89 Q90 Y92
• Binding residue (residue number reindexed from 1): G9 K10 F21 E23 K24 K62 R65 K68 R76 R77 K78 R82 K83 K84 G85 H86 R87 Q88 Y90

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6yef, PDBe:6yef, PDBj:6yef
• PDBsum: 6yef
• PubMed: 32852796
• UniProt: Q2FXS8|RL21_STAA8 Large ribosomal subunit protein bL21 (Gene Name=rplU)