Structure of PDB 6o8y Chain U Binding Site BS01

Receptor Information

>6o8y Chain U (length=89) Species: 1351 (Enterococcus faecalis)

ELLDVIKRPVITEKSMLAMDEKKYTFEVDTRANKTLVKQAVESAFDVKVA
NVNILNVRPKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQ

Ligand information

>6o8y Chain A (length=2897)

uuaagugaauaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacuaacaccgauaugcuuuggggagcuguaaguaagcuauga
uccagagauuuccgaaugggggaacccaauaucuuuuauaggauauuacu
uuucagugaauacauagcugauuagagguagacgcagagaacugaaacau
cuuaguaccugcaggaagagaaagaaaauucgauucccugaguagcggcg
agcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguuguag
gacuccgauaugguagucuguuaguauaguugaaggauuuggaaaauucc
gcuaaagagggugaaagccccguagacgaaaugcugacaacaccuaggag
gauccugaguacggcggaacacgagaaauuccgucggaauccgcggggac
caucccgcaaggcuaaauacucccuagugaccgauagugaaccaguaccg
ugagggaaaggugaaaagcaccccggaaggggagugaaauagauccugaa
accgugugccuacaacaagucaaagcucguuaaugagugauggcgugccu
uuuguagaaugaaccggcgaguuacgauugcaugcgagguuaagucgaag
agacggagccgcagcgaaagcgagucugaauagggcgaaugaguauguag
ucguagacccgaaaccaugugaucuacccauguccagguugaaggugcgg
uaaaacgcacuggaggaccgaacccacguacguugaaaagugcggggaug
agguguggguagcggagaaauuccaaacgaacuuggagauagcugguucu
cuccgaaauagcuuuagggcuagccucggaauugagaaugauggagguag
agcacuguuuggacuaggggcccaucggguuaccgaauucagauaaacuc
cgaaugccauucauuuauauccgggagucagacugcgagugauaagaucc
guagucgaaagggaaacagcccagaccaccagcuaaggucccaaaauaua
uguuaaguggaaaaggaugugggguugcacagacaacuaggauguuggcu
uagaagcagccaccauuuaaagagugcguaauagcucacuagucgaguga
cccugcgccgaaaauguaccggggcuaaacauauuaccgaagcuguggac
uacaccauuagguguagugguaggagagcguucuaagggcguugaagguc
gaucgugaggacggcuggagcgcuuagaagugagaaugccgguaugagua
gcgaaagacaggugagaauccuguccaccguaugacuaagguuuccuggg
gaaggcucguccgcccaggguuagucgggaccuaagccgaggccgauagg
cguaggcgauggacaacagguugauauuccuguaccaguuguuuuuguuu
gagcaauggagggacgcaguaggcuaaggaaugcaugcgauuggaagugc
auguccaagcaaugagucuugaguagaguuaaaugcuuuacucuuuaagg
acaaguugugacggggagcgaaauaguagcgaaguuccugacgucacacu
gccaagaaaagcuucuagugagaaaacaacugcccguaccguaaaccgac
acagguagucgaggagaguauccuaaggugagcgagcgaacucucguuaa
ggaacucggcaaaaugaccccguaacuucgggagaaggggugcugacuuc
ggucagccgcagugaauaggcccaagcgacuguuuaucaaaaacacaggu
cucugcaaaaucguaagaugaaguauaggggcugacgccugcccggugcu
ggaagguuaagaggauggguuagcuucggcgaagcucagaauugaagccc
caguaaacggcggccguaacgguccuaagguagcgaaauuccuugucggg
uaaguuccgacccgcacgaaaggcguaacgauuugggcacugucucaacg
agagacucggugaaauuuuaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccauggagcuuuacuguaguuugauauugaguguuu
guaccacauguacaggauagguaggagccgaugagaccggaacgcuaguu
ucggaggaggcgcuggugggauacuacccuuguguuaugaacccucuaac
ccgcaccacuaaucguggugggagacagugucagaugggcaguuugacug
gggcggucgccuccuaaaagguaacggaggcgcccaaagguucccucaga
augguuggaaaucauucgaagaguguaaaggcagaagggagcuugacugc
gagaccuacaagucgagcagggacgaaagucgggcuuagugauccggugg
uuccgcauggaagggccaucgcucaacggauaaaagcuacccuggggaua
acaggcuuaucucccccaagaguccacaucgacggggagguuuggcaccu
cgaugucggcucgucgcauccuggggcuguagucggucccaaggguuggg
cuguucgcccauuaaagcggcacgcgagcuggguucagaacgucgugaga
caguucggucccuauccgucgcgggcguuggaaauuugagaggagcuguc
cuuaguacgagaggaccgggauggacuuaccgcugguguaccaguuguuc
ugccaagggcauugcuggguagcuauguagggaagggauaaacgcugaaa
gcaucuaagugugaagcccaccucaagaugagauuucccauuucuuuaag
aaaguaagaccccugagagaugaucagguagauagguuggaaguggaagg
cuagugauaguuggagcggaccaauacuaaucggucgaggacuuaac

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
..<<<<<.........>>>>>..>>.>>....<<<<<......<......
>.......>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<......>>>>>>>......<<
<<<<<<.....<<<.<<.<<<<<<<<<.<<<...<<<.<......>.>>>
((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>..>>>>
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..)))......]].>>>>>>>.]]...............<<.
...>>....]].........<<<<....>>>>.............}....
>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<
........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<<.....
..>>...>>>...<<....>>...<<<.....>>>.......>>>>>>>>
>>>>>..<<.<...<<<.<<.....<<<<<<<<<..<<<....<<<<<<.
.....>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>...
.>>>>>>>>>...<<<.....>>>......>>.>>>......>.>>..<<
<<<<....<<<<<....>>>>><<<<<<<<..<..<<<<<<<<<......
.....<<<<<<<<.......<<<...>>>........>>>>>>>>.....
......>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>
>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<
<<<...........<<..<<<<<<<<<........<<.....<...<...
..........>...>..........................>>....>>>
>>>>>>.>>...........))).....>>>>>>.>>>...>>>>>>><<
<<<<<<....>>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<
<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......
<<....<<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<
...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>
>...>>>...>>...<<<<<........>>>>>))<<<<<<<........
..<<...<<<<....<<<<...<...<<<<<.........[.[.......
....<...<<<<<..<<<<.<<<<<<<<<.....>>>>>>>>>.....>>
>>..>>>>>....>...<<.........>>....>>>>>....>....>>
>>.........>>>>..>>......>>>>>>>>>>>.>>>.....<<...
...>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<
<.........<.....<<<<<....<<..>>.....>>>>><<<<<<...
.>>>>>>............<<<<<<<<<..<<<.........>>>..<<<
<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<
<<..<<<...<<...<<<<<..<<....>>...>>>>>...>>...>>>>
>>>>..>>>>><<<<<...>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.((......<<<<.((<<<<..<<<<<.<<<<<<<<<.<<<.<<<<
<<<.<<<<...........<<<<<..<<<.......<<<<<<<....>>>
>>>>....>>>............>>>>>..>>>>.>>>>>>>.>>>....
<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.
....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<
<<<<.......>>>>>>.....<<.((.((>>....>>>>>>>...<<<<
..<<<......>>>..>>>>.<<<....>>>.>>))))......<<<<<<
<.((..>>>>..))>>>>>>>.............>>>>..))>>>>....
...<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>..
.....<.<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<
<.....>>>>>.....>>>>>..>...>>>..>....<<<<<<<....>>
>.>>>>....))..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<
<<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<
<.].]>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<....
.......>>>>>....>>>.>>>>.......>>>>>>>..<<<......>
>>.....<<<<<<<<<.......>>>>>....<<<<<<<<..<<....<<
<<<....>>>>>...>>...>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6o8y Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
• Resolution: 4.14 Å
• Binding residue (original residue number in PDB): T13 E14 K15 K24 R32 N34 K35 T36 L37 K39 N52 N54 I55 L56 R59 M65 Y68 G70 Y71 K73 K74 R76 K77
• Binding residue (residue number reindexed from 1): T12 E13 K14 K23 R31 N33 K34 T35 L36 K38 N51 N53 I54 L55 R58 M64 Y67 G69 Y70 K72 K73 R75 K76

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6o8y, PDBe:6o8y, PDBj:6o8y
• PDBsum: 6o8y
• PubMed: 33004869
• UniProt: A0A1B4XKR7