Structure of PDB 6fai Chain U Binding Site BS01

Receptor Information

>6fai Chain U (length=103) Species: 559292 (Saccharomyces cerevisiae S288C)

IIKIRITLTSTKVKQLENVSSNIVKNAEQHNLVKKGPVRLPTKVLKISTR
KTPNGEGSKTWETYEMRIHKRYIDLEAPVQIVKRITQITIEPGVDVEVVV
ASN

Ligand information

>6fai Chain 2 (length=1775)

aucugguugauccugccaguagucauaugcuugucucaaagauuaagcca
ugcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcucau
uaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuca
uaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaa
uuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuc
aacggguaacggggaauaaggguucgauuccggagagggagccugagaaa
cggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccua
auucagggagguagugacaauaaauaacgauacagggcccauucgggucu
uguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauuggag
ggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguaua
uuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguug
gccggucggauuuccaacggggccuuuccuucuggcuaaccuugaguccu
uguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguuc
aaagcaggcguauugcucgaauauauuagcauggaauaauagaauaggac
guuugguucuauuuuguugguuucuaggaccaucguaaugauuaauaggg
acggucgggggcaucaguauucaauugucagaggugaaauucuuggauuu
auugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaau
caagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucuu
aaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugaccc
acucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguaug
gucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggagu
ggagccugcggcuuaauuugacggggaaacucaccagguccagacacaau
aaggauugacagauugagagcucuuucuugauuuugugggugguggugca
uggccguucuuaguugguggagugauuugucugcuuaauugcgauaacga
acgagaccuuaaccuacuaaauaguggugcuagcauuugcugguuaucca
cuucuuagagggacuaucgguuucaagccgauggaaguuugaggcaauaa
caggucugugaugcccuuagacguucugggccgcacgcgcgcuacacuga
cggagccagcgagucuaaccuuggccgagaggucuugguaaucuugugaa
acuccgucgugcuggggauagagcauuguaauuauugcucuucaacgagg
aauuccuaguaagcgcaagucaucagcuugcguugauuacgucccugccc
uuuguacacaccgcccgucgcuaguaccgauugaauggcuuagugaggcc
ucaggaucugcuuagagaagggggcaacuccaucucagagcggagaauuu
ggacaaacuuggucauuuagaggaacuaaaagucguaacaagguuuccgu
aggugaaccugcggaaggaucauua

..<<<<<...[[[[>>>>>[[[[.((((((................[[[.
[[[..<<....<<....<<..........>>...>>.>>......{{...
.....[[[..{{..{{....[[[..................<.....<<.
<<.......>>.>>......>.........<<<<<..<<....>>..>>>
>>....((((((......<<<<<<<<<<<<..>>>>....<<......>>
)))))).........<<<<..<<<.....>>>.>>>>....]]]...}}}
}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>...
...>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<........
..>>>.>>.<.<<....>>.>...]]]]]].........<<<....<<<.
....>>>..>>>...............<<<<<<<<<<<<......>>>>>
>>>>.>>>......<<..<...........>..>>.........<<<<<(
(......<<<<.........)).........>>>>.>>>>>..))))))]
]]].........[[[{{.......{{...[[[.{{....<<<<<<.<<<<
<....<..>....>>>>>.>>>>>>.....<<<<<.<..<<..<<<<<..
...>>>>>..>>..>.>>>>>....<<<<<.<<.......<<...<....
...>..<<<.....>>>....>>......>>.>>..>>>.........[[
[..((((((((....>>>>>>>>.)))))))).]]].....}}....<<<
<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>......>.
>>>>>>..>>......<<<....>>>..>>>>...>>>>>.>>>...]]]
...}}......<<<<<<<...<...<<<<.........>>>>...>>>>>
>>>..........<<<.<<.<<<..<<<<<<<<.<<<........>>>>>
>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<......<
<<<....>>>>....>>>..]]]]....<<<.<<<<<<<..<<.<<<<<<
..<<<.<<<<<<......<<.>>..((......<<<<<.<....<<<<<.
.......<<.<<<........>>>.>>......>>>>>......<<<.<<
<<<<<<<<....<<<.<<<<<....>>>...<<<......>>>...>>.>
>>....<<<<..<<..<<<<..<<<<<.<<<<<<....>>>>>>...>>>
>>..>>>>.>>....<<<<<<.....>>>>>>........>>>>....>>
>.>>>>.>>>..>>>.......>.>>>>>...>>.>>>>.>>>.....<<
<<<<<......<<.....<<..<<<<....>>>>..>>....>>......
.>>>>>>>......<....<<<<<<..........>>>>>>....>....
.>>>>>>....<<<<<<<<...))..>>>>>>>>......>>...>>>>>
>>>>>....<<<...<<.<..<<<<.<<..<<<<<<<<<<.<<<..<.<<
..<...<<<<<<<.<<<<..<<<....>>.>.>>>>.>>>>>>>...>..
>>.>..>>>.>>>>>>>>>>.>>.>>>>...>.>>......>>><<<<<<
<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6fai Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): R23 V31 K53 V56 R57 L58 P59 T60 S66 R68 N72 W79 R85 H87 R89 Y90
• Binding residue (residue number reindexed from 1): R5 V13 K35 V38 R39 L40 P41 T42 S48 R50 N54 W61 R67 H69 R71 Y72

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6fai, PDBe:6fai, PDBj:6fai
• PDBsum: 6fai
• PubMed: 29459436
• UniProt: P38701|RS20_YEAST Small ribosomal subunit protein uS10 (Gene Name=RPS20)