Structure of PDB 8hfr Chain Tl Binding Site BS01

Receptor Information

>8hfr Chain Tl (length=160) Species: 559292 (Saccharomyces cerevisiae S288C)

MGKSHGYRSRTRYMFQRDFRKHGAVHLSTYLKVYKVGDIVDIKANGSIQK
GMPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSK
CRQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQT
LAPVPYETFI

Ligand information

>8hfr Chain 1e (length=3274)

ugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagcg
gcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaauu
uggagagggcaacuuuggggccguuccuugucuauguuccuuggaacagg
acgucauagagggugagaaucccguguggcgaggagugcgguucuuugua
aagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggug
guaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaag
uacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguac
gugaaauuguugaaagggaagggcauuugaucagacgccagcaucaguuu
ugguggcaggauaaauccauaggaauguagcuugccucgguaaguauuau
agccugugggaauacugccagcugggacugaggacugcgacguaagucaa
ggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaag
gagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaaug
aaagugaacguagguuggggccucgcaagaggugcacaaucgaccgaucc
ugaugucuucggauggauuugaguaagagcauagcuguugggacccgaaa
gauggugaacuaugccugaauagggugaagccagaggaaacucuggugga
ggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguauagg
ggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuucc
cucaggauagcagaagcucguaucaguuuuaugagguaaagcgaaugauu
agagguuccggggucgaaaugaccuugaccuauucucaaacuuuaaauau
guaagaaguccuuguuacuuaauugaacguggacauuugaaugaagagcu
uuuagugggccauuuuugguaagcagaacuggcgaugcgggaugaaccga
acguagaguuaaggugccggaauacacgcucaucagacaccacaaaaggu
guuaguucaucuagacagccggacgguggccauggaagucggaauccgcu
aaggaguguguaacaacucaccggccgaaugaacuagcccugaaaaugga
uggcgcucaagcguguuaccuauacucuaccgucaggguugauaugaugc
ccugacgaguaggcaggcguggaggucagugacgaagccuagaccguaag
gucgggucgaacggccucuagugcagaucuuggugguaguagcaaauauu
caaaugagaacuuugaagacugaaguggggaaagguuccacgucaacagc
aguuggacguggguuagucgauccuaagagauggggaagcuccguuucaa
aggccugauuuuaugcaggccaccaucgaaagggaauccgguuaagauuc
cggaaccuggauauggauucuucacgguaacguaacugaauguggagacg
ucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuaucac
cccggaauugguuuauccggagauggggucuuauggcuggaagaggccag
caccuuugcuggcuccggugcgcuugugacggcccgugaaaauccacagg
aaggaauaguuuucaugccaggucguacugauaaccgcagcaggucucca
aggugaacagccucuaguugauagaauaauguagauaagggaagucggca
aaauagauccguaacuucgggauaaggauuggcucuaagggucggguagu
gagggccuuggucagacgcagcgggcgugcuuguggacugcuuggugggg
cuugcucugcuaggcggacuacuugcgugccuuguuguagacggccuugg
uaggucucuuguagaccgucgcuugcuacaauuaacgaucaacuuagaac
ugguacggacaaggggaaucugacugucuaauuaaaacauagcauugcga
uggucagaaagugauguugacgcaaugugauuucugcccagugcucugaa
ugucaaagugaagaaauucaaccaagcgcggguaaacggcguggauuaac
gagauucccacugucccuaucuacuaucuagcgaaaccacagccaaggga
acgggcuuggcagaaucagcggggaaagaagacccuguugagcuugacuc
uaguuugacauugugaagagacauagaggguguagaauaagugggagcuu
cggcgccagugaaauaccacuaccuuuauaguuucuuuacuuauucaaug
aagcggagcuggaauucauuuuccacguucuagcauucaaggucccauuc
ggggcugauccggguugaagacauugucagguggggaguuuggcuggggc
ggcacaucuguuaaacgauaacgcagauguccuaaggggggcucauggag
aacagaaaucuccaguagaacaaaaggguaaaagcccccuugauuuugau
uuucagugugaauacaaaccaugaaaguguggccuaucgauccuuuaguc
ccucggaauuugaggcuagaggugccagaaaaguuaccacagggauaacu
ggcuuguggcagucaagcguucauagcgacauugcuuuuugauucuucga
ugucggcucuuccuaucauaccgaagcagaauucgguaagcguuggauug
uucacccacuaauagggaacgugagcuggguuuagaccgucgugagacag
guuaguuuuacccuacugaugaauguuaccgcaauaguaauugaacuuag
uacgagaggaacaguucauucggauaauugguuuuugcggcugucugauc
aggcauugccgcgaagcuaccauccgcuggauuauggcugaacgccucua
agucagaauccaugcuagaacgcggugauuucuuugcuccacacaauaua
gauggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcu
agcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugc
aaugucauuuugcguggggauaaaucauuuguauacgacuuagauguaca
acgggguauuguaagcaguagaguagccuuguuguuacgaucugcugaga
uuaagccuuuguugucugauuugu

.......................<<<<<<.....<<....>>......>>
>>>.>.........<<....>>..<<<<<......<<.....>>.....>
>>>>..<<<..<<.......<<.<<<<<<...>>>>>>.>>.......>>
....<<<<<<.<<<<<....<<<<<<<<<<<<<....<<<<......>>>
>.(.......<<<......)>>>..>.>>>>>>>>>..>>>........>
>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<<
..((((((>>>>>..>>>................................
..<<....>>...............<<<<....>>>>.............
.....................<<<<<.<<<<<....<<<<<<<.<<<<<<
<<<<<<<<<.....<<<<<<<<<......<<<<........>>>>.....
..>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>..
...>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<<
....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<<.
...>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<
...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<..<
<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>...
..>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>...
<<.......>>......>>>>>>...>..>.>>..<<<<<<....<.<<<
....>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<.........
..........<<<<<.....>>>>>................>>>.....>
>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>>.>>>>>
>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>.
..<<<<<<<..<<<<(((.....<<<<<<.....<<..........<<<.
<<<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>..
.......<<......>>...>>>>>...>>>>>>>>>>>>>.........
.))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>
>>>>>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>
>>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<
<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<..
...>>.>>>>>>....((<<<<<<...<<....<<<....>>>.......
..<<..............>>.....>>....>>...<<<<<........>
>>>>))<<<<.<<<<<<<.....<<<....>>>.......<<<<<<...<
<<<.<<<<<<<.<<<<<<<<<<..[[[..>>>>>><...<<<<<....<<
<<<<....<<<.....>>>....>>>>>>.....>>>>>....><<<<<<
<<....>>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>..
........>>>..>>>>>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.........<
.....<<<<<....<<..>>.....>>>>><<.<<<<<<<<.........
<<.....................<<<<<<<........<<<<.......<
.....>......>>>>........>>>>>>>...................
..<<<<<.....>>>>>.....>>..............>>..>>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<.....>>>>......>>>>>>>...<<<.<<<..<<<<<..<..
<<<.....<<<<....>>>>.....>>>>.>>>..>>>>>..>>>....>
>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<...
.....>>>>>....<<<<<..<<<........<<<<<.<<<<<<..<<<<
<.<<<<<<<<..<<<<<<<<<<<<<<..<...........<<........
..................>>..>..>>>>.>>>>>>>>>>...<<<<<..
...<<<<..<<<<<......>>>>>...............<<<<<<....
>>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....
>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<
<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<
<<.......>>>>.>>>>.<<<....>>>.>>)))......<<<<<<<..
<<<<.))))))>>>>>>>>>>>.........>>>>..>>>>>>>......
........<<<<<....<.......>....>>>>>.............((
...<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<<<....
.))..>>>.....>>>>>..>...>>>>......<<<<<<<....>>>.>
>>>........>>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<...
............>>>>>>.><<<<<...<<<<....<<<<<<<<<<]]].
>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........
>>>>>...>>>>.>>>>....>>>>>>>>.......<<<<<..<<<....
...<<..........<<<<<<.....>>>>>>......>>.......<<<
<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>.......
.........>>>..>>>>>..<<<<<.<.............>.....<<<
<<<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>
>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8hfr Nuclear export of pre-60S particles through the nuclear pore complex.
• Resolution: 2.64 Å
• Binding residue (original residue number in PDB): M1 G2 S4 H5 G6 Y7 R8 S9 R10 R12 Q16 R17 H22 G23 K35 S47 Q49 H54 Y57 G59 T61 K69 K78 V80 N82 R83 K87 R88 N90 R92 R102 F105 R108 N112 R116 Q127 K129 R130
• Binding residue (residue number reindexed from 1): M1 G2 S4 H5 G6 Y7 R8 S9 R10 R12 Q16 R17 H22 G23 K35 S47 Q49 H54 Y57 G59 T61 K69 K78 V80 N82 R83 K87 R88 N90 R92 R102 F105 R108 N112 R116 Q127 K129 R130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8hfr, PDBe:8hfr, PDBj:8hfr
• PDBsum: 8hfr
• PubMed: 37258668
• UniProt: Q02753|RL21A_YEAST Large ribosomal subunit protein eL21A (Gene Name=RPL21A)