Structure of PDB 7ls1 Chain T2 Binding Site BS01

Receptor Information

>7ls1 Chain T2 (length=135) Species: 10090 (Mus musculus)

GKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPR
KVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVF
RDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Ligand information

>7ls1 Chain A2 (length=3707)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccgcgcgucgcggcgugggaaau
guggcguacggaagacccacuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcucgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcgcguccggccgugccggugg
uccggcggaucuuucccgcgcgggggaccgcccccggccggcgaccggcc
gccgccgggcgcacuuccaccguggcggugcgccgcgaccggcuccggga
cggccgggaaggcccgguggggaagguggcucggggggggcucaccccga
guguuacagcccuccggccgcgcuuuucgccgaaucccggggccgaggaa
gcccgucgccgcgcucucccucucccgggugggggccgggccgccccucc
cacggcgcgaccgcucggggcggacuguccccagugcgccccgggcgucg
ucgcgccgucgggucccgggggcgucggucacgcgugacgaagccgagcg
cacggggucggcggcgaugucggcuacccacccgacccgucuugaaacac
ggaccaaggagucuaacgcgugcgcgagucaggggcucguccgaaagccg
ccguggcgcaaugaaggugaagggccccgcccgggggcccgaggugggau
cccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgccc
gccgcgccggggagguggagcacgagcguacgcguuaggacccgaaagau
ggugaacuaugcuugggcagggcgaagccagaggaaacucugguggaggu
ccguagcgguccugacgugcaaaucggucguccgaccuggguauaggggc
gaaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuc
aggauagcuggcgcucucgcuacgcaguuuuauccgguaaagcgaaugau
uagaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaug
gguaagaagcccggcucgcuggcguggagccgggcguggaaugcgagugc
cuagugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaa
cgccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggu
guugguugauauagacagcaggacgguggccauggaagucggaauccgcu
aaggaguguguaacaacucaccugccgaaucaacuagcccugaaaaugga
uggcgcuggagcgucgggcccauacccggccgucgccggcagucggaacg
gaacgggacgggagcggcgucgggccccgcggagccuacgccgcgacgag
uaggagggccgcugcggugagccuugaagccuagggcgcgggcccgggug
gagccgccgcaggugcagaucuuggugguaguagcaaauauucaaacgag
aacuuugaaggccgaaguggagaaggguuccaugugaacagcaguugaac
augggucagucgguccugagagaugggcgagugccguuccgaagggacgg
gcgauggccuccguugcccucggccgaucgaaagggagucggguucagau
ccccgaauccggaguggcggagaugggcgccgcgaggcguccagugcggu
aacgcgaccgaucccggagaagccggcgggagccucggggagaguucucu
uuucuuugugaagggcagggcgcccuggaauggguucgccccgagagagg
ggcccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucu
cgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggcc
guacccauauccgcagcaggucuccaaggugaacagccucuggcauguug
gaacaauguagguaagggaagucggcaagccggauccguaacuucgggau
aaggauuggcucuaagggcugggucggucgcggccggcgccuagcagccg
acuuagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaa
gcaucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccag
ugcucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcg
ggaguaacuaugacucucuuaagguagccaaaugccucgucaucuaauua
gugacgcgcaugaauggaugaacgagauucccacugucccuaccuacuau
ccagcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaa
gaagacccuguugagcuugacucuagucuggcacggugaagagacaugag
agguguagaauaagugggaggcccggccgccggugaaauaccacuacucu
caucguuuuuucacugacccggugaggcgggggggcgagccccgaggggc
ucucgcuucuggcgccaagcguccgucccgcgcgugcgggcgggcgcgac
ccgcuccggggacagugccagguggggaguuugacuggggcgguacaccu
gucaaacgguaacgcagguguccuaaggcgagcucagggaggacagaaac
cucccguggagcagaagggcaaaagcucgcuugaucuugauuuucaguac
gaauacagaccgugaaagcggggccucacgauccuucugaccuuuugggu
uuuaagcaggaggugucagaaaaguuaccacagggauaacuggcuugugg
cggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcuc
uuccuaucauugugaagcagaauucaccaagcguuggauuguucacccac
uaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuu
acccuacugaugauguguuguugccaugguaauccugcucaguacgagag
gaaccgcagguucagacauuugguguaugugcuuggcugaggagccaaug
gggcgaagcuaccaucugugggauuaugacugaacgccucuaagucagaa
ucccgcccaggcggaacgauacggcagcgccgaggagccucgguuggccc
cggauagccggguccccguccugccccgcggccgggcgucgggaccgggg
uccggugcggagagccguucgucuugggaaacggggugcggccggaaagg
gggccgcccucucgcccgucacguugacgcacguucguguggaaccuggc
gcuaaaccauucguagacgaccugcuucugggucgggguuucguacguag
cagagcagcucccucgcugcgaucuauugaaagucagcccucgacacaag
gguuugu

.........................<<<<<<<....<......>.....>
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>>>>>>..[[[..<.<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>.>..<<<<<<<<<....<<<<<<<<.<<<....<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>>>>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<..<......<<<<<<<<<....<<<.<<<.<<<<..<<
....<<<<.<<<..<<<<<>>>>>>>>>>>>>>.>>>>>>>....>>>..
>>>>>>>>>........>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<.
<<.....<<.<<<<.<<.<.<<......<<<<<<<<.<<..>>.>>>>>>
>>........>>>>>>>>>.......>>..>>...>>>>>>>>>>.....
..........<<<<<<.<<<<<.<<..>>.>>>>>...<<<..<<<<.<<
<<<<<<<<<<<<<<<<<<<<<<<...........>.>>>>>>>>>>>..>
>>>>>>>>>.>>>....>>>>..>>>.....<<.....>>.....>>>>>
>......>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>
>><<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>.
....>>>...<<....>>...<<<<<<<.....>>>>>>>..<<<<<...
<<<..<<<...(((.[[..>>>..>>>....>>>>>.<<<<<<<<<.<<<
<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<
<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>
>>...<<<..<..<<........>>.>.>>>....>>>>>>>>>...<<.
......>>......>>>>>>...>..>.>>..<<<<<<....<.<<<...
.>>>.><<<<<<<<<<.......<<<.<<<<<<<<<.<...<....<<<.
...<<<<.....<<..........>>..>>>>....>>>..>.....>>>
>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>
>.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>..
.<<<<<<<...<<<(((.......<<<<<.....<<..........<<<.
<<<<<<<<<<.......<<<<<.<<....<<.........>>....>>..
.......<<......>>...>>>>>...>>>>>>>>>>>>>.........
.))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........
..]].)))...<<<...>.>>..................>>>>>>>>>..
..>>>>>>.<.<<<<<<<..<<......<<<<.<<<<....>>>>>>>>.
..>>.>>>>>>>..>.....>>>>>><<<....<<.....<<<<<<....
...>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>
>>>>....((<<<<<<...<<....<<<....>>>..........<<.<<
<<<.<<<...>>>.>>>>>>>......>>....>>...<<<<<.......
.>>>>>))<<<<....<<<<<...<<<<<<<....>>>>>>>...<<<..
..>>>...<..<<<<<<<...<<<<<<<<<<<<..<<<<<<<<<..{{{.
.>>>>>>....<<<<<.<<<<.<<<......<<<.....>>>......>>
>>>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>....>>>>>
>>>>>>>.........>>>>>>>..>..........>>.>>>.>>>>>>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<
<<.....<<<<<<<.<<.........<.....<<<<<....<....>...
..>>>>><<.<<<<<<<<<<<...<..........>........>>>>>.
.>>>>>>.>>............<<<<<<<<<..<<<.........>>>..
<<<<<<<....<<<<.....>>>>......>>>>>>>....<<.<<<..<
<<<<<....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>>.
<<<<<.......>>>>>.........<...........<<<<<.......
>>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<........>>>>>....[[[[[..{{{.{{
......[[[[.{{[[[[..<<<<<.<<<<<<<<<.<<<<<<<<<<<<..<
<<...........<..............................>.>>>.
.>>.>>>>>>>>>>...<<<<<.....<.<<<....<<<<.<<...>>>>
>>................<<<<<<<.<<<<<....>>>>>>>>>>>>..>
>>>..>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<
<<...........>>>>>>..<<....<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>....<<<..<<<<.......
>>>>..>>>.<<<....>>>.>>))).......((((((..(((..>>>>
>>.)))))))))..........]]]]..}}]]]].......<<.<.<.<<
<<<<...<<.......>>...>>>>>>...>>..>>.......<<<<<.<
<<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}
..}}}.]]]]].....<<<<<<..<.<<<..<.<<<<<............
.....>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>
>.>>...>>>>...>>>><<<<....<<<<<...........>>>>>...
.>>>>.>>>...>.>>>>>>.....<<<<<<.<<<..<<.<....<<<..
......<<<<<<.<<<<<..<<<.....<<<>>>.....>>.>..>>>>>
>>>>>>.......>>>...<<<..<<.<....<<<<.<<<<<<.......
.>>>>>>>>>>....>>>..>>>...............>.>>..>>>>>>
>>><<<<<.<.............>....<<.<<<<<<<.<<.....<<<<
....<<<<<......>>>>>...>>>>....>>....>>>>.>>>.>>..
>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ls1 Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): A15 G16 R17 R36 P37 Y38 R48 R51 T54 A55 G58 K59 K64 R65 K67 V74 Y75 N76 N78 H79 K107 R111 R112 K115 K133 R135 F136
• Binding residue (residue number reindexed from 1): A14 G15 R16 R35 P36 Y37 R47 R50 T53 A54 G57 K58 K63 R64 K66 V73 Y74 N75 N77 H78 K106 R110 R111 K114 K132 R134 F135

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0140236 translation at presynapse
• GO:0140242 translation at postsynapse
• GO:1904044 response to aldosterone

Cellular Component

• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005791 rough endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:0098556 cytoplasmic side of rough endoplasmic reticulum membrane
• GO:0098793 presynapse
• GO:0098794 postsynapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ls1, PDBe:7ls1, PDBj:7ls1
• PDBsum: 7ls1
• PubMed: 34815424
• UniProt: P61358|RL27_MOUSE Large ribosomal subunit protein eL27 (Gene Name=Rpl27)