Structure of PDB 7r81 Chain T1 Binding Site BS01

Receptor Information

>7r81 Chain T1 (length=191) Species: 5141 (Neurospora crassa)

VNLRTQKRLASSVLGVGQRKIWLDPNEVSEISNANSRQTIRKLVADGLII
KKPVTMHSRSRARELNLARRIGRHRGFGKRKGTADARMPEQVLWMRRQRV
LRRLLVKYRASGKIDKHLYHELYHLAKGNTFKHKRALVEHIHRAKAEKAR
EKAIKEEMDAKRARTKAARERKLERQAAKRNALAGEEEESK

Ligand information

>7r81 Chain A1 (length=3123)

uugaccucggaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaacagggauugcccuaguaacggcgagugaagc
ggcaacagcucaaauuugaaaucuggcuucggcccgaguuguaauuugua
gaggaagcuuuuggugaggcaccuucugaguccccuggaacggggcgcca
uagagggugagagccccguauagucggaugccgauccaauguaaagcucc
uucgacgagucgaguaguuugggaaugcugcucaaaaugggagguaaauu
ucuucuaaagcuaaauaccggccagagaccgauagcgcacaaguagagug
aucgaaagaugaaaagcacuuugaaaagaggguuaaauagcacgugaaau
uguugaaagggaagcguuugugaccagacuugcgccguuccgaucauccg
guguucucaccggugcacucgggacggcucaggccagcaucgguuuuggc
ggggggauaaagguccggggaacguagcuccuccgggaguguuauagccc
cgggcguaaugcccucgccgggaccgagguucgcgcaucugcaaggaugc
uggcguaauggucaucaacgacccgucuugaaacacggaccaaggaguca
agguuuugcgcgaguguuuggguguaaaacccgcacgcguaaugaaagug
aacguaggugagagcuucggcgcaucaucgaccgauccugauguauucgg
auggauuugaguaagagcguuaagccuuggacccgaaagauggugaacua
ugcuuggauagggugaagccagaggaaacucugguggaggcucgcagcgg
uucugacgugcaaaucgaucgucaaaucugagcaugggggcgaaagacua
aucgaaccaucuaguagcugguuaccgccgaaguuucccucaggauagca
guguuguucuucaguuuuaugagguaaagcgaaugauuagggaccauucu
caaacuuuaaauauguaagaagcccuuguuacuuaauugaacgugggcau
ucgaauguaccaacacuagugggccauuuuugguaagcagaacuggcgau
gcgggaugaaccgaacgcgggguuaaggugccggaguggacgcucaucag
acaccacaaaaggcguuaguacaucuugacagcaggacgguggccaugga
agucggaauccgcuaaggacuguguaacaacucaccugccgaauguacua
gcccugaaaauggauggcgcucaagcgucccacccauaccccgcccucag
gguagaaacgaugcccugaggaguaggcggccguggaggucagugacgaa
gccuagggcgugagcccgggucgaacggccucuagugcagaucuuggugg
uaguagcaaauacuucaaugagaacuugaaggaccgaaguggggaaaggu
uccaugugaacagcgguuggacauggguuagucgauccuaagccauaggg
aaguuccguuucaaaggggcagugccccguguggcgaaagggaagccggu
uaacauuccggcaccuggauguggguuuugcgcggcaacgcaacugaacg
cggagacgacggcgggggccccgggcagaguucucuuuucuucuuaacgg
ucuaucacccugaaaacaguuugucuggagauaggguuuaauggccggaa
gagcccgacacuucugucggguccggugcgcucucgacgucccuugaaaa
uccgcgggagggaauaauucucacgccaggucguacucauaaccgcagca
ggucuccaaggugaacagccucugguugauagaacaauguagauaaggga
agucggcaaaauagauccguaacuucgggaaaaggauuggcucuaagggu
uggacaaccaacuuagaacugguacggacagggggaaucugacugucuaa
uuaaaacauagcauugcgauggccagaaagugguguugacgcaaugugau
uucugcccagugcucugaaugucaaagugaagaaauucaaccaagcgcgg
guaaacggcgggaguaacuaugacucucuuaagguagccaaaugccucgu
caucuaauuagugacgcgcaugaauggauuaacgagauucccacuguccc
uaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauc
agcggggaaagaagacccuguugagcuugacucuaguuugacauugugaa
aauuuuuuuacuuauucaauuaagcggggcuggauuuucguccaacuucu
gguuuuaagguccuucgcgggccgacccggguugaagacauugucaggug
gggaguuuggcuggggcggcacaucuguuaaaccauaacgcagguguccu
aaggggggcucauggagaacagaaaucuccaguagaacaaaaggguaaaa
guccccuugauuuugauuuucagugugaauacaaaccaugaaaguguggc
cuaucgauccuuuagucccucgaaauuugaggcuagaggugccagaaaag
uuaccacagggauaacuggcuuguggcggccaagcguucauagcgacguc
gcuuuuugauccuucgaugucggcucuuccuaucauaccgaagcagaauu
cgguaagcguuggauuguucacccacuaauagggaacgugagcuggguuu
agaccgucgugagacagguuaguuuuacccuacugaugaccucaucgcaa
ugguaauugagcuuaguacgagaggaaccgcucauucagauaauugguuu
uugcgguuguccgaccgggcagugccgcgacgcuaccaucugcuggauaa
uggcugaacgccucuaagucagaauccaugccagaacgcgaugauaccuc
cagcacguuguagacguauaagaauaggcuccggccucguaucuuagcag
gcgauuccuccguuggccuugaaguggccagcgguaauucgcguauugua
auuuuaacacgcgcuggaucaaauccuuugcagacgacuuagaugugcga
aaggguccuguaagcaguagaguagccuuguuguuacgaucugcugaggg
uaagcccuccuucgccuagauuu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<..<<.......<<.<<<....>>>.>>.......>>....
<<<<.<<<<<.<<<...<<<<<<..<<....<<<<......>>>>.((..
....<<<.....))>>>....>>..>>.>>>>...>>>.....>>>>>.>
>>>........<<<<<<<........>>>>>>>.....<<<<<..(((((
(>>>>>..>>>..................................<<...
.>>...............<<<<....>>>>....................
..............<<<<.<<<<<<.....<<.<<<<<<<....<....<
<....>>....>........>>>.>>>>.>>.<<<<<<<.<<<<<<<<<<
<<<<<.....<.<<<<<<<<......<<<<<....>>>>>.......>>>
>>>>>....>.>>>>>>>>>>>>>>>........<<<..>>>.....>>>
>>>>.....>>>>>>.>>>>..<<<<........>>>><<<<<<....<<
<<<<<<.<<<<..<<<..<<<<<.....>>>>>..>>>...<<....>>.
......<<<<<..<<....>>...>>>>>.<<<<<<<<...<<....>>.
..>>>.>>>.>>....>>>>.>>>>>>>>..<<.<..<<<<<<<.....<
<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<.
.<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>.
.....>>>>>>...>..>.>>...<<<<<....<.<<<....>>>.><<<
<<<<<<.....<<.<<<<<<<.....<<<....<<<..<.<<.>>.>..>
>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.>>>>..
...>>>>...>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>...
.<<<......>>>...<<<<<<<...<<<(((.....<<<<<<......<
..........<<<.<<<<<<<<<<.......<<<<<.<<<...<<.....
....>>...>>>.........<........>...>>>>>...>>>>>>>>
>>>>>..........))).>....>>>>>>.>>>...>>>>>>><<<<<<
<<<.........>>>>>>>>>....>>>>>.<.<<<<<<<<.<<......
<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<
.....<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<..
.<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<<...<<<
....>>>........<<<<<<.>>>>>>....>>>....>>...<<<<<.
.......>>>>>))<<<<.<<<<.<<<....<<<....>>>......(<<
<<<<...<<<<.<<<<<<<<<<<<<.<<<<.......>>>>.><...<<<
<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>...
.><<<<<<<<....>>>>>>>>>>>>.>.>>>>>>>.>>>>.........
>>>>>>.........>>>...>>>>>>>>>>>>..>>.....<<......
>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<..
.......<.....<<<<<....<<..>>.....>>>>><<.<<<<<<<<<
<<..>>>>>..>>>>>>.>>............<<<<<<<<<..<<<....
.....>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>...
<<..<<<..<<<<<<....<<<....<<<<<....>>>>.>...>>>.>>
>>..>>>>><<<<<<.......>>>>>>........<...........<<
<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>..>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<
<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<..<<<<<
<....>>>>>>...<<<<<.....<<<<..<<<<......>>>>......
....)...<<<<<.....>>>>>..>>>>..>>>>>.....>>>>>>>>.
.>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<<.
..<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>....
>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)
))......<<<<<<<..<<<<.))))))>>>>>>>>>>>.........>>
>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>>...>>>
>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>
>>>>>...>.<<<.........>>>.....>>>>>..>...>>>.>>...
.<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<<<<<<..
<<<<.<.<<<<<.................>>>>>.><<<<<...<<<<..
..<<<<<<<<<<....>>>>>..>>>>>...>>>>..>>>>>.<<<<...
<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>....<<
<<<<.<<<.<<......<<.......<<<....>>>....>>........
<<<<.....<<<<<<<<<.......>>>>>>>>>....>>>>........
......>>>>>.>>>>>>..<<.<.<<.............>>....<.<<
.<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>
....>>>>..>>..>...>.>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7r81 Structure of the translating Neurospora ribosome arrested by cycloheximide
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): V2 N3 R5 T6 G18 Q19 R20 K21 W23 L24 P26 S37 R38 Q39 R42 K43 A46 T56 M57 H58 S59 R60 S61 R62 A63 R64 R70 R71 R74 H75 R76 G77 G79 R81 K82 G83 T84 D86 R88 P90 Q92 W95 M96 R100 V101 R103 R104 R110 H118 Y120 H121 Y124 H125 L126 K128 G129 N130 H134 K135 R136 H143
• Binding residue (residue number reindexed from 1): V1 N2 R4 T5 G17 Q18 R19 K20 W22 L23 P25 S36 R37 Q38 R41 K42 A45 T55 M56 H57 S58 R59 S60 R61 A62 R63 R69 R70 R73 H74 R75 G76 G78 R80 K81 G82 T83 D85 R87 P89 Q91 W94 M95 R99 V100 R102 R103 R109 H117 Y119 H120 Y123 H124 L125 K127 G128 N129 H133 K134 R135 H142

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7r81, PDBe:7r81, PDBj:7r81
• PDBsum: 7r81
• PubMed: 34815343
• UniProt: Q7S5N9