Structure of PDB 8htz Chain T Binding Site BS01

Receptor Information

>8htz Chain T (length=86) Species: 562 (Escherichia coli)

ANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAF
NEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA

Ligand information

>8htz Chain A (length=1519)

aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacga
guggcggacgggugaguaaugucugggaaacugccugauggagggggaua
acuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagagg
gggccucuugccaucggaugugcccagaugggauuagcuaguaggugggg
uaacggcucaccuaggcgacgaucccuagcuggucugagaggaugaccag
ccacacuggaacugagacacgguccagacuccuacgggaggcagcagugg
ggaauauugcacaaugggcgcaagccugaugcagccaugccgcguguaug
aagaaggccuucggguuguaaaguacuuucagcggggaggaagggaguaa
aguuaauaccuuugcucauugacguuacccgcagaagaagcaccggcuaa
cuccgugccagcagccgcgguaauacggagggugcaagcguuaaucggaa
uuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugugaaau
ccccgggcucaaccugggaacugcaucugauacuggcaagcuugagucuc
guagagggggguagaauuccagguguagcggugaaaugcguagagaucug
gaggaauaccgguggcgaaggcggcccccuggacgaagacugacgcucag
gugcgaaagcguggggagcaaacaggauuagauacccugguaguccacgc
cguaaacgaugucgacuuggagguugugccggcguggcuuccggagcuaa
cgcguuaagucgaccgccuggggaguacggccgcaagguuaaaacucaaa
ugaauugacgggggcccgcacaagcgguggagcaugugguuuaauucgau
gcaacgcgaagaaccuuaccuggucuugacauccacggaaguuuucagag
augagaaugugccuucgggaaccgugagacaggugcugcauggcugucgu
cagcucguguugugaaauguuggguuaagucccgcaacgagcgcaacccu
uauccuuuguugccagcgguccggccgggaacucaaaggagacugccagu
gauaaacuggaggaagguggggaugacgucaagucaucauggcccuuacg
accagggcuacacacgugcuacaauggcgcauacaaagagaagcgaccuc
gcgagagcaagcggaccucauaaagugcgucguaguccggauuggagucu
gcaacucgacuccaugaagucggaaucgcuaguaaucguggaucagaaug
ccacggugaauacguucccgggccuuguacacaccgcccgucacaccaug
ggaguggguugcaaaagaaguagguagcuuaaccuucgggagggcgcuua
ccacuuugugauucaugacuggggugaagucguaacaagguaaccguagg
ggaaccugcgguuggauca

.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<...<<<<<<<<<......>>>>>>.>>>.>..
>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<.<<....
.<<<<<......>>>>>......>>.>.....<<<....>>>....<<<<
<<.>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<..
.......>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>>>
>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>>.
........<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<<<.
.....<<<<<..>>>>>.....>>>>...<.<<<<<......<.<<<<<<
<<.......>>>>>>>>.>........>>>>>....>..<<<<<(((...
<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>....
......<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<.....
.<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<<<
<<...<<<<<<<....<<<<<<<<...<<<......>>>......>>>>>
>>>...........<<....>>.>>>>>>>..>>>>.>>>>...>>>...
>>>>....<<<<<<...<<...<<<<.<.....>.>>>>...>>>>>>>>
..........<<<<<<.<<<<<<<<<<<<..>>>>>>>>>>>>..<<..)
)>>.....>>>>>>.>>>.<<<......<<<<....>>>>....>>>..)
))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<..
......>>..........<<<<<<<......<<<<<<<..<<<<<<<...
.>>>>>>>.............>>>>>.>>.<<<.<<<..<<<<<<.....
..<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.....<<<
<.<<<<<<<...<<..<<<.....>>>>>....>>>>>>>.....<<<<<
.....>>>>>........>>>>.........>>>...>>>>>>>>>...>
>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<<...<<<
....>>>...>>....>>>.....>>>>>>>......<....<<<<<<<.
.......>>>>>>>....>.....>>>>>>....<<<<<<<.........
>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<
<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>>>>>>>>
>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<
....>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8htz Structural insights into the decoding capability of isoleucine tRNAs with lysidine and agmatidine
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): A2 N3 I4 K5 S6 K9 R10 K16 A17 R18 N21 S23 R24 S26 R29 T30 K33 Y36 Q55 P56 D59 K64 H68 N70 K71 A73 R74 K76 A77
• Binding residue (residue number reindexed from 1): A1 N2 I3 K4 S5 K8 R9 K15 A16 R17 N20 S22 R23 S25 R28 T29 K32 Y35 Q54 P55 D58 K63 H67 N69 K70 A72 R73 K75 A76

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008073 ornithine decarboxylase inhibitor activity
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8htz, PDBe:8htz, PDBj:8htz
• PDBsum: 8htz
• PubMed: 38538915
• UniProt: P0A7U7|RS20_ECOLI Small ribosomal subunit protein bS20 (Gene Name=rpsT)